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| GENE EXPRESSION TECHNOLOGY, 185
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Volume 185: Gene Expression Technology
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John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
David Goeddel, Genentech, Inc., Molecular Biology Department, South San Francisco, California, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Description
The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs
and will prove useful in solving the majority of expression problems one is likely to encounter.
Audience
Biochemists, geneticists, microbiologists, genetic engineers, analytical chemists, molecular biologists, biotechnologists, and industrial researchers working on protein products.
Contents
Introduction:
D.V. Goeddel, Systems for Heterologous Gene Expression.
Expression in Escherichia
coli:
L. Gold, Expression of Heterologous Proteins in Escherichia coli.
Vectors:
P.
Balbas and F. Bolivar, Design and Construction of Expression Plasmid Vectors in Escherichia coli.
Promoters:
J.D. Gralla, Promoter Recognition and mRNA Initiation by Escherichia coli Eo-70.D.G. Yansura and D.J.
Henner, Use of Escherichia coli trp Promoter for Direct Expression of Proteins.F.W. Studier, A.H. Rosenberg, J.J.
Dunn, and J.W. Dubendorff, Use of T7 RNA Polymerase to Direct Expression of Cloned Genes.
Translation:
L. Gold
and G.D. Stormo, High-Level Translation Initiation.B.E. Schoner, R.M. Belagaje, and R.G. Schoner, Enhanced Translational
Efficiency with Two-Cistron Expression System.H.A. de Boer and A.S. Hui, Sequences within Ribosome Binding Site Affecting Messenger
RNA Translatability and Method to Direct Ribosomes to Single Messenger RNA Species.P.O. Olins and S.H. Rangwala, Vector for
Enhanced Translation of Foreign Genes in Escherichia coli.
Proteases:
S. Gottesman, Minimizing Proteolysis
in Escherichia coli: Genetic Solutions.
Gene Fusions:
M. Uhlén and T. Moks, Gene Fusions for
Purpose of Expression: An Introduction.B. Nilsson and L. Abrahmsén, Fusions to Staphylococcal Protein A.D.G. Yansura,
Expression as trpE Fusion.
Secretion:
J.A. Stader and T.J. Silhavy, Engineering Escherichia coli
to Secrete Heterologous Gene Products.
Protein Refolding:
T. Kohno, D.F. Carmichael, A. Sommer, and R.C. Thompson,
Refolding of Recombinant Proteins.
Expression in Bacillus subtilis:
D.J. Henner, Expression of Heterologous
Genes in Bacillus subtilis.
Intracellular Production:
S.F.J. Le Grice, Regulated Promoter for High-Level
Expression of Heterologous Genes in Bacillus subtilis.
Secretion:
V. Nagarajan, System for Secretion
of Heterologous Proteins in Bacillus subtilis.
Inducible Expression of Regulatory Proteins:
D.J. Henner,
Inducible Expression of Regulatory Genes in Bacillus subtilis.
Expression in Yeast:
S.D. Emr, Heterologous
Gene Expression in Yeast.
Plasmid Vectors:
A.B. Rose and J.R. Broach, Propagation and Expression of Cloned
Genes in Yeast: 2-?Circle-Based Vectors.T.Stearns, H. Ma, and D. Botstein, Manipulating Yeast Genome Using Plasmid Vectors.
Inducible Expression Cassettes:
L.M. Mylin, K.J. Hofmann, L.D. Schultz, and J.E. Hopper, Regulated GAL4
Expression Cassette Providing Controllable and High-Level Output from High-Copy Galactose Promoters in Yeast.V.L. Price, W.E. Taylor,
W. Clevenger, M. Worthington, and E.T. Young, Expression of Heterologous Proteins in Saccharomyces cerevisiae Using ADH2
Promoter.T. Etcheverry, Induced Expression Using Yeast Copper Metallothionein Promoter.
Constitutive Expression Cassettes:
S.M. Kingsman, D. Cousens, C.A. Stanway, A. Chambers, M. Wilson, and A.J. Kingsman, High-Efficiency Yeast Expression Vectors
Based on the Promoter of the Phosphoglycerate Kinase Gene.S. Rosenberg, D. Coit, and P. Tekamp-Olson, Glyceraldehyde-3-phosphate
Dehydrogenase-Derived Expression Cassettes for Constitutive Synthesis of Heterologous Proteins.A.Z. Sledziewski, A. Bell, C. Yip,
K. Kelsay, F.J. Grant, and V.L. MacKay, Superimposition of Temperature Regulation on Yeast Promoters.
Translation:
T.F. Donahue and A.M. Cigan, Sequence and Structural Requirements for Efficient Translation in Yeast.
Proteases:
E.W. Jones, Vacuolar Proteases in Yeast Saccharomyces cerevisiae.K.D. Wilkinson, Detection and Inhibition
of Ubiquitin-Dependent Proteolysis.R.L. Kendall, R. Yamada, and R.A. Bradshaw, Cotranslational Amino-Terminal Processing.
Protein
Secretion and Modification:
A.J. Brake, à-Factor Leader-Directed Secretion of Heterologous Proteins from Yeast.
R.A. Hitzeman, C.Y. Chen, D.J. Dowbenko, M.E. Renz, C. Liu, R. Pai, N.J. Simpson, W.J. Kohr, A. Singh, V. Chisholm, R. Hamilton,
and C.N. Chang, Use of Heterologous and Homologous Signal.
Sequences for Secretion of Heterologous Proteins from Yeast.C.E.
Ballou, Isolation, Characterization, and Properties of Saccharomyces cerevisiae mnn Mutants with Nonconditional Protein
Glycosylation Defects.V. Chisholm, C.Y. Chen, N.J. Simpson, and R.A. Hitzeman, Molecular and Genetic Approach to Enhancing
Protein Secretion.
Expression in Mammalian Cells:
A.D. Levinson, Expression of Heterologous Genes in Mammalian
Cells.
Vectors:
R.J. Kaufman, Vectors Used for Expression in Mammalian Cells.M. Kriegler, Assembly
of Enhancers, Promoters, and Splice Signals to Control Expression of Transferred Genes.
Transfection Methods:
W.A.
Keown, C.R. Campbell, and R.S. Kucherlapati, Methods for Introducing DNA into Mammalian Cells.
Markers for Selection and
Amplification:
R.J. Kaufman, Selection and Coamplification of Heterologous Genes in Mammalian Cells.
Growth
of Cell Lines:
J.P. Mather, Optimizing Cell and Culture Environment for Production of Recombinant Proteins.
Posttranslation
Processing, Modification, and Secretion:
A.J. Dorner and R.J. Kaufman, Analysis of Synthesis, Processing, and Secretion
of Proteins Expressed in Mammalian Cells.
Mutagenesis:
B.H. Horwitz and D. DiMaio, Saturation Mutagenesis Using
Mixed Oligonucleotides and M13 Templates
Containing Uracil.
Author Index.
Subject Index.
| Bibliographic details |
Hardbound, 681 pages, publication date: MAY-1990
ISBN-13: 978-0-12-182086-2
ISBN-10: 0-12-182086-6
Imprint: ACADEMIC PRESS
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