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Using Nature for the Art of Expression
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Edited By
Joseph Fernandez, Invitrogen Corporation, Carlsbad, California, U.S.A.
James Hoeffler, Invitrogen Corporation, Carlsbad, California, U.S.A.
Description
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene
expression systems from an array of organisms. It describes several different types of expression systems including transient, stable,
viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression
system, and provides an overview of the future of recombinant protein expression.
Audience
Molecular Biologists, Biochemists, Protein Chemists, and Biophysicists.
Contents
Contributors.
Introduction: The Art of Expression.
Purpose of This Book.
Selecting a Suitable Expression System:
Considerations.
Genomics
and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler.
Prokaryotic Expression Systems:
Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin.
Expression Vectors Employing the trc Promoter, J. Brosius.Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller.araB Expression
System in Escherichia coli, M. Better.
Eukaryotic Expression Systems:
Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow.
Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg.
Recombinant
Protein Expression in Pichia methanolica, C.K. Raymond.
Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F.
Stinski.
Inducible Mammalian Expression Systems, M.Russell.
Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett.
Expression in Insect Systems:
Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman.
Baculovirus Expression Vector System, M. Galleno and A.J. Sick.
Transgenic
Expression:
Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson.
Expression of Recombinant
Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E.
Smith, and J.M. Curling.
Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy.
Index.
| Bibliographic details |
Hardbound, 480 pages, publication date: NOV-1998
ISBN-13: 978-0-12-253840-7
ISBN-10: 0-12-253840-4
Imprint: ACADEMIC PRESS
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| Price and Ordering |
Price:
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USD 103
GBP 64
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Last update: 4 Sep 2009
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