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| ANALYTICAL TECHNIQUES
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Edited By
T.P. Mommsen, University of Victoria, BC, Canada
Peter Hochachka, University of British Columbia, Vancouver, British Columbia, Canada
Included in series
Biochemistry and Molecular Biology of Fishes, 3
Description
This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be
invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers
and students with a pertinent information source from theoretical and experimental angles.
Contents
Preface. Contributors. Abbreviations.
Anaesthetics (G.K. Iwama, P.A. Ackerman). Cannulation techniques (M. Axelsson,
R. Fritsche). Surgery: selective islet removal and total pancreatectomy (A. Epple, B. Nibbio). Hypophysectomy of fish (R.S. Nishioka).
Surgical extirpation of the Stannius corpuscles: stanniectomy (J.C. Fenwick). Microsurgery: gonadectomy (E. McLean). Hepatectomy (Y.
Inui). Injection techniques (S.F. Perry, S.G. Reid). Techniques for recording from electric organs (H.H. Zakon). Intact and demembranated
muscle fibres (J.D. Altringham, I.A. Johnston). Isolated perfused fish hearts (W.R. Driedzic, J.R. Bailey). Methods for assessing kidney
and urinary bladder function in fish (C.M. Wood, M.L. Patrick). Cell lines: availability, propagation and isolation (N.C. Bols, L.E.J.
Lee). Extracorporeal circulation (S. Thomas). Perifusion of pancreatic islets (S.L. Milgram, J.K. McDonald, B.D. Noe). Perfusion of pancreatic
endocrine tissue of teleost fish (P. Ronner). The isolated rete (E.A. Rasio, M. Bendayan, C.A. Goresky). Teleost pituitary cells: Isolation,
culture and use (J.P. Chang, R.M. Jobin). Photophores: the analysis of bioluminescent systems (J.-F. Rees, E.M. Thompson). Opercular
membranes and skin (S.D. McCormick). Isolation of gill cells (P.M. Verbost, G. Flik, H. Cook). Testes cells: isolation and culture (M.
Loir, P. Sourdaine). Primary culture of rainbow trout corpuscles of Stannius (B. Gellersen, G.F. Wagner). Isolated cardiac myocytes (C.L.
Milligan). Oogenesis (H. Kagawa). Adipose tissue (M.A. Sheridan, J.S. Harmon). Enzymes of lipogenesis (H. Segner, R. Böhm). Isolation
and culture of head kidney macrophages (R.A. Garduño, W.W. Kay). In vitro culture of fish leukocytes (N.W. Miller, E. Churchill
McKinney). Hepatocytes: isolation, maintenance and utilization (T.P. Mommsen, T.W. Moon, P.J. Walsh). Organ culture of fish tissues (P.A.
Janssens, J.A. Grigg). Hepatocyte perifusion (C. Ottolenghi et al.). Intracellular pH (P.J. Walsh). Measurement of intracellular
calcium in fish liver cells (T.W. Moon). Xenobiotics: designing an in vitro system to study enzymes and metabolism (C.J. Kennedy). Peptide
receptor assays: insulin receptor (J. Gutiérrez, E.M. Plisetskaya). Thyroid hormone: deiodinase and receptor assays (O. Bres,
D.L. MacLatchy, J.G. Eales). Steroid receptors in fish: membrane and intracellular preparations (M. Weisbart et al.). Membrane,
cell preparation and receptor assay — catecholamines (S.D. Reid, S.F. Perry). Membrane preparation and receptor assays —
adenosine (J.F. Siebenaller, T.F. Murray). Fish mitochondria (J.S. Ballantyne). Peroxisomes and peroxisomal Β-oxidation (C.D. Moyes,
E.L. Crockett). Use of membrane vesicle techniques to characterize nutrient transport processes of the teleost gastrointestinal tract
(G.A. Ahearn, C. Storelli). Incorporation of 35S-sulphate into branchial cartilages: a biological model to study hormonal
regulation of skeletal growth in fish (C. Duan). Development of radioimmunoassay for a model peptide hormone: insulin (E.M. Plisetskaya).
Radioimmunoassay for growth hormones, prolactins and somatolactins (P. Swanson). Hormone measurement by ELISA: application to fish gonadotrophin
(O. Kah). Developing an ELISA for a model protein — vitellogenin (J.L. Specker, T.R. Anderson). Bolus-injection of radiolabels
in the study of steady state glucose metabolism (T.G. West). In vivo nuclear magnetic resonance spectroscopy of aquatic animals
(A. van Waarde, G. van den Thillart). Analysis of enzyme regulation via reversible phosphorylation and enzyme binding interactions with
macromolecules (K.B. Storey). Metabolite transport in fish red blood cells and hepatocytes (T.W. Moon, P.J. Walsh). Ca2+ transport
across plasma membranes (G. Flik, P.M. Verbost). Analyzing phosphatidylinositol cycling and protein kinase C involvement (F.W. Goetz).
Appendix: Buffered salt solutions, culture media and tissue homogenization buffers (T.P. Mommsen, P.W. Hochachka).
Species Index.
Subject Index.
| Bibliographic details |
Hardbound, 0 pages, publication date: OCT-1994
ISBN-13: 978-0-444-82032-7
ISBN-10: 0-444-82032-9
Imprint: ELSEVIER
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