1.Measuring Chlamydomonas IFT (Dentler) 2. Purification of IFT Particle Proteins from Chlamydomonas and preparation of Recombinant
proteins for in vitro functional analysis and structural studies (Cole) 3. Biochemical and functional analysis of IFT motors and particles
in C. elegans (Scholey) 4. Functional genomics of IFT and BBSome in C.elegans (Leroux) 5. IFT analysis in Danio (Drummond) 6.
Scanning EM of cilia and organs in mice (Pazour) 7. Imaging the mouse embryonic node (Bruekner) 8. Measuring nodal flow (Hirokawa) 9.
Measuring ciliary induced Ca2+ influxes in cultured kidney cells (Praetorius) 10. Analysis of Cargo transport by IFT and GFP imaging
of IFT in Chlamydomonas (Diener/Rosenbaum) 11. GFP imaging of IFT in mammalian primary cilia (Shah) 12. Markers for neuronal
cilia (Mykytyn) 13. Preservation and staining of human respiratory epithelial cells Immunofluoresence localization of mammalian dyneins
to distinct axonemal locations (Omran) 14. Purification of the BBS complex (Nachury) 15. Use of IFT Cre-Lox mutants to disrupt
cilia in specific mouse organs (Yoder) 16. Genetic approaches to IFT analysis in Chlamydomonas including RNAi (Iomini/Dutcher) 17.
Planarians as a model system for analysis of basal body components (Marshall) 18. Analysis of IFT particle size by TIRF microscopy
(Marshall) 19. EM tomography of IFT particles (Lupetti/Pigino) 20. IFT analysis in Drosophila (Kernan) 21. Role of IFT in development
of rod outer segment (Besharse) 22. Immunoprecipitation of IFT complexes(Pazour) 23. Analysis of Hedgehog signaling downstream
of IFT mutants (IF and in situ hybridization of hedgehog targets) (Eggenschwiler) 24. IFT particle-based signaling in Chlamydomonas
(Wang/Snell)
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