Audience
Researchers in cell, molecular and developmental biology; biochemists pharmacologists, and geneticists
Contents STRUCTURE AND FUNCTION
1. A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies
(Andrew D. Luster
and Gabriele Campanella)
2. Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors (Micheal R. Farzan)
3. Lymphotactin
Structural Dynamics (Brian F. Volkman)
4. Regulation of chemokines by posttranslational modifications (Paul Proost )
5. Proteolytic Processing
of Chemokines: Mass Spectrometry, Biochemistry, Neoepitope Antibody and Functional Characterization (Christopher Overall and Amanda Starr)
6. Multiple approaches to study Chemokine Receptor Homo and Heterodimerization (Mario Mellado)
7. Heterodimerization of Chemokine Receptors
with Other GPCRs (Graeme Milligan and Shirley Appelbe)
8. Homo and Hetero-Oligomerization of Chemokines (Tracy Handel, Damon Hamel, Ariane
Jansma)
9. Interactions of Chemokine with Glycosaminoglycans (Amanda Proudfoot)
10. Interactions of Chemokine with Glycosaminoglycans
(Andreas J. Kungl)
11. Structures of Chemokine:Chemokine Binding Protein Complexes (Daved Fremont)
12. Activation Mechanisms of Chemokine
Receptors (Mette Rosenkilde)
13. Modeling Small Molecule Compound Binding to Receptors (Richard Horuk, James Pease, N. Vaidehi)
14. Spectroscopic
Approaches to Study Conformational Changes in GPCRs (David Farrens)
15. Plasmon Resonance Methods in GPCR Signaling (Victor Hruby, Isabel
Alves, Gordon Tollin)
16. Flow Cytometric Analyses of GPCR Interactions or Rapid-mix flow cytometry measurements of subsecond regulation
of G protein-coupled receptor ternary complex dynamics (Larry Sklar)
17. Expression and Functional Reconstitution of CCR1 (Tracy Handel
and Samantha Allen, Damon Hamel)
18. Characterization of the Interactions of Chemokines with Glycosaminoglycans by Fourier Transform
Ion Cyclotron Resonance (Julie A. Leary)
CHEMOKINE FUNCTION IN CELL MIGRATION
19. In vitro assays for studying chemokine-triggered lymphocyte
transendothelial migration under physiological flow conditions (Ronen Alon and Francis Lin)
20. Microfluidic device (Eugene Butcher)
21. Two-photon imaging and quantitative analysis of leukocyte migration in vivo (Mark Miller and Jenna Cash)
22. Zymosan induced peritonitis
as a model for studying chemokines and inflammatory mediators in vivo (David Greaves)
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