Description The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly
recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume
413) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols
and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of
formation, and biological activities of this important class of protein assemblies.
Audience
Biochemists, cell biologists, neuroscientists, and related fields engaged in protein research.
Contents
Characterization of protein deposition in vivo and ex vivo.
Chapter 1: PMCA for diagnosis and prion propagation studies.
Chapter 2: Fractionation of prion protein aggregates by asymmetrical flow field-flow fractionation.
Chapter 3: Analysis of Amyloid Aggregates
Using Agarose Gel Electrophoresis.
Chapter 4: Characterization of Systemic Amyloid Deposits by Mass Spectrometry.
Chapter 5: Proteomics
of Polyglutamine Aggregates.
Chapter 6: Merger of Laser Capture Microdissection and Mass Spectrometry: A Window into the Amyloid Plaque
Proteome.
Chapter 7: MALDI MS Imaging of Amyloid.
Chapter 8: Imaging polyglutamine deposits in brain tissue.
Chapter 9: X-34 labeling
of abnormal protein aggregates during the progression of Alzheimer's disease.
Chapter 10: Visualizing Pathology Deposits in the Living
Brain of Alzheimer's Disease Patients.
Chapter 11: Micro-Imaging of Amyloid in Mice
Cell and animal models of amyloid formation
and toxicity
Chapter 12: An efficient protein transformation protocol for introducing prions into yeast.
Chapter 13: Screening
for genetic modifiers of amyloid toxicity in yeast.
Chapter 14: Searching for anti-prion compounds: Cell-based high-throughput in vitro
assays and animal testing strategies.
Chapter 15: A Drosophila Model of Alzheimer's Disease.
Chapter 16: A C. elegans model of polyglutamine
disease.
Computational Approaches and Theory
Chapter 17: Nucleation: the Connections between Equilibrium and Kinetic
Behavior.
Chapter 18: Computational Approaches to Amyloid beta Fibril Core Structure.
Chapter 19: Amyloid beta-protein aggregations:
Ab initio discrete molecular dynamics approaches.
Chapter 20: Computational approaches to fibril structure and formation
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