Edited by
Alan Wolffe, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, U.S.A.
Series Editor:
John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Description
This volume of
Methods in Enzymology includes up-to-date procedures used for the assembly of nucleosomes, chromatin,
and nuclei, extending the classical procedures described nearly ten years ago in
Volume 170 (Nucleosomes) of this series,
and should assist in the further investigation of the ways in which the structural dynamics of chromatin contribute to geh regulation
of transcription, replication, recombination, and repair. Also described in this volume are assay for the structure and function of
in vitro reconstituted chromatin and for defining the organization and characteristics of natural chromosomal material from yeast
(Saccharomyces cerevisae), flies (Drosophila melanogaster), and frogs (Xenopus laevis), as well as from mammalian tissues. The purification
and assay procedures for various chromatin remodeling activities, including histone acetyltransferases, histone deacetylases, and SWI/SNF
ATPases are detailed.
The critically acclaimed laboratory standard for more than forty years,
Methods in Enzymology
is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains
much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Included in series
Methods in Enzymology
Audience:
Graduate students and research scientists involved in biochemistry, molecular biology, cell biology, genetics, developmental biology, and biophysics.
