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| MEMBRANE PROTEIN PURIFICATION AND CRYSTALLIZATION
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A Practical Guide
To order this title, and for more information, click here
Second Edition
Edited By
Carola Hunte, Max-Planck-Institut fuer Biophysik, Frankfurt, Germany
Gebhard von Jagow, Zentrum F r Biologische Chemie, Frankfurt, Germany
Hermann Schagger, Zentrum F r Biologische Chemie, Universit tsklinikum Frankfurt, Germany
Description
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists
working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques
in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides
protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This
edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches
for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.
Audience
Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry
Contents
I. Strategies and Techniques
Purification Strategies for Membrane Proteins.
Chromatographic Techniques and Basic Operations
in Membrane Protein Purification.
Production and purification of recombinant membrane proteins.
SDS Electrophoresis Techniques.
Blue-Native
Electrophoresis.
Preparative Isoelectric Focussing.
Membrane protein crystallization.
II. Discussion of Selected Isolation
Protocols
Lipid Dependent Inactivation and Reactivation of Bovine complex III.
Purification of affinity-epitope tagged G-protein
coupled receptors.
Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D
Crystallization.
Purification of the
yeast cytochrome bc1 complex.
III. Crystallization of Membrane Proteins
Antibody fragment-mediated crystallization
of membrane proteins.
Crystallization of Wolinella succinogenes quinol:fumarate reductase.
Ba3-type cytochrome-c oxidase from Thermus
thermophilus:
purification, crystallization, and crystal transformation.
Two-dimensional crystallization of membrane proteins: a practical
guide.
In cubo crystallization of membrane proteins.
| Bibliographic details |
Spiral Bound, 316 pages, publication date: DEC-2002
ISBN-13: 978-0-12-361776-7
ISBN-10: 0-12-361776-6
Imprint: ACADEMIC PRESS
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| Price and Ordering |
Price:
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EUR 77.95
USD 105
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Last update: 30 Nov 2009
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