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Edited By Kreitzer Geri Julio Celis, Danish Cancer Society; Institute of Cancer Biology and Danish Centre for Human Genome Research; Copenhagen, Denmark
Audience
Graduate students and professionals in a lab enviornment
Contents
Protein Determination
Phosphopeptide Mapping: A Basic Protocol
Coupling of Fluorescent Tags to Proteins
Radioiodination of Proteins and Peptides
Free-Flow Electrophoresis
Gel-Based Proteomics: High-Resolution Two-Dimensional
Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.
High-Resolution Two-Dimensional
Electrophoresis with Immobilized pH Gradients for Proteome Analysis
Two-Dimensional Difference Gel Electrophoresis: Application
for the Analysis of Differential Protein Expression in Multiple Biological Samples
Affinity Electrophoresis for Studies of Biospecific
Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal
Antibodies in Murine Blood Plasma
Image Analysis and Quantitation
Fluorescence Detection of Proteins in Gels Using
SYPRO Dyes
Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples
Two-Dimensional
Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope Labeling
Immunoprecipitation of Proteins under Nondenaturing
Conditions
Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the
Analysis of Mitochrondrial Oxidative Phosphorylation Complexes
Affinity purification with Natural Immobilized Ligands
Analysis
of Protein-Protein Interactions by Chemical Cross-Linking
Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions
Biomolecular Interaction Analysis Mass Spectrometry
Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to
Mapping Protein-Protein Interaction Sites
Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins
Modular
Scale Yeast Two-Hybrid Screening
Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite Green
Chromatin Immunoprecipitation (ChIP)
Gel Mobility Shift Assay
DNA Affinity Chromatography of Transcription Factors:
The Oligaonucleotide Trapping Approach
Protein Degradation Methods: Chaperone-Mediated Autophagy
Methods in Protein
Ubiquitination
Protein Identification and Sequencing by Mass Spectrometry
Proteome Specific Sample Preparation Methods
for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
In-Gel Digestion of Protein Spots for Mass Spectrometry
Peptide Sequencing by Tandem Mass Spectrometry
Direct Database Searing Using Tandem Mass Spectra of Peptides
Identification
of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching Tools
Identification of Protein Phosphorylation Sites by Mass Spectrometry
Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry
Stable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative Proteomics
Site-Specific, Stable Isotopic Labeling
of Cysteinyl Pepties in Complex Peptide Mixtures
Protein Hydrogen Exchange measured by Electrospry Ionization Mass Spectrometry
Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide
Mixtures
Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
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