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Gerard Marriott, University of Wisconsin, U.S.A. Ian Parker, University of California, Irvine
Description The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly
respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised
by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still
relevant today—truly an essential publication for researchers in all fields of life sciences.
Contents Fluorescence: Basic Concepts, Practical Aspects, and Some Anecdotes.
Fluorescence Sensing Methods.
Bioluminescence and Chemiluminescence.
Fluorescent Amino Acid Analogs.
Fluorescent Nucleotides: Synthesis and Characterization.
Photophysics of Green and Red Fluorescent
Proteins: Implications for Quantitative Microscopy.
Development of Genetically Encoded Fluorescent Indicators for Calcium.
Development
and Application of Caged Calcium.
Application of Fluorescent Probes to Study Mechanics and Dynamics of Ca2+-Triggered Synaptotagmin
C2 Domain-Membrane Interactions.
Caging Proteins through Unnatural Amino Acid Mutagenesis.
Preparation and Light-Directed Activation
of Caged Proteins.
Bioluminescence Resonance Energy Transfer: Monitoring Protein-Protein Interactions in Living Cells.
Structure-Function
Relationships in Metalloproteins.
Spectroscopy and Microscopy of Cells and Cell Membrane Systems.
Photonics for Biologists.
Imaging
at Low Light Levels with Cooled and Intensified Charge-Coupled Device Cameras.
Filters and Mirrors for Applications in Fluorescence
Microscopy.
Resolution in Optical Microscopy.
Video-Rate Confocal Microscopy.
Giant Vesicles, Laurdan, and Two-Photon Fluorescence
Microscopy: Evidence of Lipid Lateral Separation in Bilayers.
Biological Near-Field Microscopy.
Fluorescence Lifetime-Resolved Imaging:
Measuring Lifetimes in an Image.
Fluorescence Resonance Energy Transfer Imaging Microscopy.
FRET Imaging Microscopy.
Fluorescence
Resonance Energy Transfer Imaging Microscopy and Fluorescence Polarization Imaging Microscopy.
Homo-FRET versus Hetero-FRET to Probe
Homodimers in Living Cells.
Spinning Disk Confocal Microscope System for Rapid High Resolution, Multimode, Fluorescence Speckle Microscopy
and Green Fluorescent Protein Imaging in Living Cells.
Single-Particle Tracking Image Microscopy.
Diffusion in Cells Measured by Fluorescence
Recovery after Photobleaching, etc.
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