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Fred Rainey, College of Basic Sciences, Louisiana State University, Baton Rouge, U.S.A. Aharon Oren, Department of Plant and Environmental Sciences, The Institute of Life Sciences, The Hebrew University of Jerusalem, Israel
Description This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation,
cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture.
It contains protocols specific for work with extremophiles, as well as adaptations of ?standard? microbiology protocols modified to enable
the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations
of ?standard? microbiology protocols that have to be modified to be suitable for handling extremophiles.
Audience
General microbiologists, industrial microbiologists, biotechnologists, molecular biologists, microbial ecologists, envirnomental and marine microbiologists
Contents
THERMOPHILES In-situ activity studies in thermal environments
The isolation of thermophiles from deep-sea hydrothermal
environments
Growth of hyperthermophilic microorganisms for physiological and nutritional studies
Toward the large scale cultivation
of hyperthermophiles at high temperature and high-pressure
The chemotaxonomic analysis of lipids from extremophilic bacteria
Membranes
of thermophiles and other extremophiles
Characterization and quantification of compatible solutes in (hyper)thermophilic microorganisms
Functional genomics of the thermo-acidophilic archaeon Sulfolobus solfataricus
Heat shock proteins in hyperthermophiles
Deep-sea
Thermococcales and their genetic elements: plasmids and viruses
Genetic systems for Thermus
Gene transfer systems for obligately
anaerobic thermophilic bacteria
Hyperthermophilic virus-host systems: detection and isolation
Preservation of thermophilic microorganisms
PSYCHROPHILES
Handling psychrophilic microorganisms
Proteins from psychrophiles
ALKALIPHILES
Cultivation
of aerobic alkaliphiles
Isolation, cultivation, and characterization of alkalithermophiles
ACIDOPHILES
The isolation
and study of acidophilic microorganisms
HALOPHILES
Characterization of natural communities of halophilic microorganisms
Cultivation of haloarchaea
Halophile extraction from ancient crystals
The assessment of the viability of halophilic microorganisms in
natural communities
Characterization of lipids of halophilic Archaea
Characterization of organic compatible solutes of halotolerant and
halophilic organisms
Genetic systems for halophilic archaea
The isolation ?of viruses of halophilic microorganisms
Detection, quantification
and purification of halocins: peptide antibiotics from haloarchaeal extremophiles
The storage of halophilic bacteria
BAROPHILES
Handling piezophilic microorganisms
RADIATION-RESISTANT MICROORGANISMS
Measuring survival in microbial populations
following exposure to ionizing radiation
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