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| MOLECULAR MOTORS AND THE CYTOSKELETON, PART B, 298
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Volume 298
To order this title, and for more information, click here
John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Richard Vallee, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Description
General Description of the Volume:
This Methods in Enzymology volume continues the description (initiated
in Volume 85) of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. It discusses some
of the more promising attempts to apply techniques of molecular biology genetics and molecular cytochemistry to these proteins. General
Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology
is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains
much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Contents
Analysis of Actomyosin-Related Systems:
R.E. Cheney, Purification and Assay of Myosin V.K. Ayscough,
Use of Latrunculin-A, An Actin Monomer Binding Drug.M.R. Bubb and I. Spector, Use of the F-Actin Binding Drugs, Misakinolide
A and Swinholide A.E.J. Luna, F-Actin Blot Overlays.J.F. Kelleker, R.D. Mullins, and T.D. Pollard, Purification and
Assay of the Arp2/3 Complex from Acanthamoeba castellani.M.D. Welch and T.J. Mitchison, Purification and Assay of
the Platelet Arp2/3 Complex.K.L. Schmeichel, B.E. Stronach, and M.C. Beckerle, Purification and Assay of Zyxin.C.E. Turner
and M.C. Brown, Purification and Assays for Paxillin.J. Lacoste, A. Ma, and J.T. Parsons, Assay and Purification of Focal
Adhesion Kinase.T. Jarchau, T. Mund, M. Reinhard, and U. Walter, Purification and Assays of VASP.J.A. Theriot and D.C.
Fung, Listeria monocytogenes-based Assays for Actin Assembly Factors.
Analysis of Microtubule-Related Systems:
A. Desai and T.J. Mitchison, Preparation and Characterization of Caged Fluorescein Tubulin.D. Meyer, D.R. Rines, A. Kashina,
and J.M. Scholey, Purification of Novel Kinesins from Embryonic Systems.D.W. Pierce and R.D. Vale, Assaying Processive
Movement of Kinesin by Fluorescence Microscopy.J.B. Bingham, S.J. King, and T.A. Schroer, Purification of Dynactin and Dynein
from Brain Tissue.M.P. Sheetz and J. Kumar, Isolation and Characterization of Kinectin.G.S. Diamantopoulos, T.E. Kreis,
and J.E. Rickard, Purification and Assay of CLIP-170.F. McNally, Purification and Assay of the Microtubule-Severing Protein,
Katanin.Y. Zheng, M.L. Wong, B. Alberts, and T. Mitchison, Purification and Assay of gTubulin Ring Complex.M. Blomberg-Wirschell
and S.J. Doxsey, Rapid Isolation of Centrosomes.G.A. Orr, S. Rao, C.S. Swindell, D.G.I. Kingston, and S.B. Horwitz, Photoaffinity
Labeling Approach to Map Taxol-Binding Site on the Microtubule.M.A. Jordan and L.Wilson, Use of Drugs to Study Role of Microtubule
Assembly Dynamics in Living Cells.
Other Cytoskeletal Systems:
K. Oegema, A. Desai, M.L. Wong, T.J. Mitchison, and
C.M. Field, Purification and Assay of A Septin Complex from Drosophila Embryos.A. Mukherjee and J. Lutkenhaus,
Purification, Assembly and Localization of FtsZ.C. Lu and H.P. Erickson, Purification and Assembly of FtsZ.
Cell-Free
and Genetic Systems:
E.D. Salmon, E. Yeh, S. Shaw, B. Skibbens, and K. Bloom, High Resolution Video and Digital Enhanced
Differential Interference Contrast Light Microscopy of Cell Division in Budding Yeast.D.A. Compton, Production of M-phase and
I-phase Extracts from Mammalian Cells.V.J. Allan, Organelle Motility and Membrane Network Formation in Metaphase and Interphase
Cell-Free Extracts.C. McGoldrick and M. Sheetz, Organelle Motility and Membrane Network Formation from Cultured Mammalian Cells.
S.L. Rogers, I.S. Tint, and V.I. Gelfand, An in Vitro Motility Assay for Melanophore Pigment Organelles.J.K. Burkhardt,
Purification of Phagosomes and Assays for Microtubule Binding.J. Scheel and T.E. Kreis, Magnetic Bead Assay for Characterization
of Microtubule-Membrane Interactions.L. Haimo, Reactivation of Vesicle Transport in Lysed Teleost Melanophores.M.P. Koonce,
U. Euteneuer, and M. Schliwa, Reactivatable Cell Models of Giant Ameba, Reticulomyxa.
Force Production Assays:
R.J. Baskin, Design and Use of the Centrifuge Microscope to Assay Force Production.Y.J. Han and J.R. Sellers, Motility
Assays on Molluscan Native Thick Filaments.A.D. Mehta, J.T. Finer, and J.A. Spudich, Use of Optical Traps in Single Molecule
Study on Nonprocessive Biological Motors.K. Visscher and S.M. Block, Versatile Optical Traps with Feedback Control.Y.
Wang and R.J. Pelham, Jr., Preparation of a Flexible, Porous Polyacrylamide Substrate for Mechanical Studies of Cultured Cells.
T. Oliver, K. Jacobson, and M. Dembo, Design and Use of Substrata to Measure Traction Forces Exerted by Cultured Cells.
General
Methods:
C.M. Field, K. Oegema, Y. Zheng, T.J. Mitchison, and C.E. Walczak, Purification of Cytoskeletal Proteins Using
Peptide Antibodies.J.E. Eriksson, D.M. Toivola, C. Sahlgren, A. Mikhailov, and A.S. Harmala-Brasken, Strategies to Assess Phosphoprotein
Phosphatase and Protein Kinase-Mediated Regulation of the Cytoskeleton.T.M. Svitkina and G.G. Borisy, Correlative Light and
Electron Microscopy of the Cytoskeleton of Cultured Cells.
Subject Index.
Author Index.
| Bibliographic details |
Hardbound, 636 pages, publication date: AUG-1998
ISBN-13: 978-0-12-182199-9
ISBN-10: 0-12-182199-4
Imprint: ACADEMIC PRESS
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