Guide for Authors

All journal information and instructions compiled in one document (PDF) in just one mouse-click Author information pack

INTRODUCTION
BEFORE YOU BEGIN
• Ethics in publishing
• Declaration of interest
• Submission declaration and verification
• Changes to authorship
• Copyright
• Role of the funding source
• Open access
• Submission
• Additional Information
PREPARATION
• Article Structure
• Introduction
• Discussion
• Experimental
• Keywords
• Abbreviations
• Nomenclature and Units
• Artwork
• Color Artwork
• Tables
• References
• Journal Abbreviations Source
• Video
• Supplementary material
• Database linking
• CONTENT INNOVATION
• AudioSlides
• Chemical Compound Viewer (Reaxys)
• 3D molecular models
• Interactive plots
• Submission checklist
• Additional Information
AFTER ACCEPTANCE
• Online proof correction
• Offprints
AUTHOR INQUIRIES



Phytochemistry invites research articles on all aspects of pure and applied plant chemistry, plant biochemistry, plant molecular biology and chemical ecology. The Journal is currently divided up into the following sections:

Editorial Comment, Molecules of Interest, Review Articles, Structural Elucidation and Full Papers.

Editorial Comment will be an occasional series where Regional Editors, Board Members or other scientists will be invited to comment on phytochemistry topics of global interest and debate.

Molecules of Interest will consist of invited short reviews (3-4) printed pages of individual compounds or macromolecules of plant, fungal or algal origin. These can be novel compounds or newly discovered properties of familiar compounds. Please contact Dr Richard J Robins if you wish to prepare a Molecules of Interest paper.

Review Articles are published at regular intervals, ranging in scope from primary metabolism and regulation of plant growth, through plant enzymology to natural product chemistry and the biological activity of plant products. They deal with significant new areas of research and are intended to command the interest of the general reader. Authors should consult their Regional Editors with an outline of their proposed Review before preparing such articles. Published Reviews include a biography and picture of each author.

Structure Elucidation papers, accepted as full papers in the Chemistry section, should include either a substantial description of several new compounds without any conclusion as to their significance, or a description of the study of new compounds with expected structures incorporating conclusions. These papers with a minimum of 16 pages of double-spaced manuscript should follow the general style of Full Papers although the Introduction, Results and Discussion may be combined as a single narrative. Brief abstracts must be included, containing significant facts derived from the work. Reports of known compounds, however rare, from new plant sources will not generally be accepted unless they have real chemotaxonomic or other biological significance. Authors are specifically discouraged from submitting papers as fragmented analyses of particular plant constituents.

Full Papers: Full journal articles will be drawn from areas described in the Aims and Scope:
Bioactive Products
Chemotaxonomy
Chemistry
Ecological Biochemistry
Metabolism
Molecular Genetics & Genomics
Protein Biochemistry & Proteomics
Update in Bioinformatics
They are comprehensive papers, typically 6-8 printed pages in length (a minimum of 20 pages of double-spaced manuscript). Papers on plant chemistry must be substantial and contain convincing justification for undertaking the study, as well as having conclusions (e.g. on the biology, chemotaxonomy, new biosynthetic pathways etc.). Papers submitted under the Bioactive Products area are unlikely to be accepted if the bioactivity is measured on a mixture of compounds without further resolution.

Ethics in publishing

Please see our information pages on Ethics in publishing and Ethical guidelines for journal publication.

Declaration of interest

All authors are requested to disclose any actual or potential conflict of interest including any financial, personal or other relationships with other people or organizations within three years of beginning the submitted work that could inappropriately influence, or be perceived to influence, their work. More information.

Submission declaration and verification

Submission of an article implies that the work described has not been published previously (except in the form of an abstract or as part of a published lecture or academic thesis or as an electronic preprint, see 'Multiple, redundant or concurrent publication' section of our ethics policy for more information), that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or in any other language, including electronically without the written consent of the copyright-holder. To verify originality, your article may be checked by the originality detection service CrossCheck.

Changes to authorship

Authors are expected to consider carefully the list and order of authors before submitting their manuscript and provide the definitive list of authors at the time of the original submission. Any addition, deletion or rearrangement of author names in the authorship list should be made only before the manuscript has been accepted and only if approved by the journal Editor. To request such a change, the Editor must receive the following from the corresponding author: (a) the reason for the change in author list and (b) written confirmation (e-mail, letter) from all authors that they agree with the addition, removal or rearrangement. In the case of addition or removal of authors, this includes confirmation from the author being added or removed.
Only in exceptional circumstances will the Editor consider the addition, deletion or rearrangement of authors after the manuscript has been accepted. While the Editor considers the request, publication of the manuscript will be suspended. If the manuscript has already been published in an online issue, any requests approved by the Editor will result in a corrigendum.

Article transfer service
This journal is part of our Article Transfer Service. This means that if the Editor feels your article is more suitable in one of our other participating journals, then you may be asked to consider transferring the article to one of those. If you agree, your article will be transferred automatically on your behalf with no need to reformat. Please note that your article will be reviewed again by the new journal. More information.

Copyright

Upon acceptance of an article, authors will be asked to complete a 'Journal Publishing Agreement' (see more information on this). An e-mail will be sent to the corresponding author confirming receipt of the manuscript together with a 'Journal Publishing Agreement' form or a link to the online version of this agreement.

Subscribers may reproduce tables of contents or prepare lists of articles including abstracts for internal circulation within their institutions. Permission of the Publisher is required for resale or distribution outside the institution and for all other derivative works, including compilations and translations. If excerpts from other copyrighted works are included, the author(s) must obtain written permission from the copyright owners and credit the source(s) in the article. Elsevier has preprinted forms for use by authors in these cases.

For open access articles: Upon acceptance of an article, authors will be asked to complete an 'Exclusive License Agreement' (more information). Permitted third party reuse of open access articles is determined by the author's choice of user license.

Author rights
As an author you (or your employer or institution) have certain rights to reuse your work. More information.

Elsevier supports responsible sharing
Find out how you can share your research published in Elsevier journals.

Role of the funding source

You are requested to identify who provided financial support for the conduct of the research and/or preparation of the article and to briefly describe the role of the sponsor(s), if any, in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. If the funding source(s) had no such involvement then this should be stated.

Funding body agreements and policies
Elsevier has established a number of agreements with funding bodies which allow authors to comply with their funder's open access policies. Some funding bodies will reimburse the author for the Open Access Publication Fee. Details of existing agreements are available online.

Open access

This journal offers authors a choice in publishing their research:

Open access
• Articles are freely available to both subscribers and the wider public with permitted reuse.
• An open access publication fee is payable by authors or on their behalf, e.g. by their research funder or institution.
Subscription
• Articles are made available to subscribers as well as developing countries and patient groups through our universal access programs.
• No open access publication fee payable by authors.

Regardless of how you choose to publish your article, the journal will apply the same peer review criteria and acceptance standards.

For open access articles, permitted third party (re)use is defined by the following Creative Commons user licenses:

Creative Commons Attribution (CC BY)
Lets others distribute and copy the article, create extracts, abstracts, and other revised versions, adaptations or derivative works of or from an article (such as a translation), include in a collective work (such as an anthology), text or data mine the article, even for commercial purposes, as long as they credit the author(s), do not represent the author as endorsing their adaptation of the article, and do not modify the article in such a way as to damage the author's honor or reputation.

Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND)
For non-commercial purposes, lets others distribute and copy the article, and to include in a collective work (such as an anthology), as long as they credit the author(s) and provided they do not alter or modify the article.

The open access publication fee for this journal is USD 3300, excluding taxes. Learn more about Elsevier's pricing policy: http://www.elsevier.com/openaccesspricing.

Green open access
Authors can share their research in a variety of different ways and Elsevier has a number of green open access options available. We recommend authors see our green open access page for further information. Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository after an embargo period. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications. Embargo period: For subscription articles, an appropriate amount of time is needed for journals to deliver value to subscribing customers before an article becomes freely available to the public. This is the embargo period and it begins from the date the article is formally published online in its final and fully citable form.

This journal has an embargo period of 24 months.

Elsevier Publishing Campus
The Elsevier Publishing Campus (www.publishingcampus.com) is an online platform offering free lectures, interactive training and professional advice to support you in publishing your research. The College of Skills training offers modules on how to prepare, write and structure your article and explains how editors will look at your paper when it is submitted for publication. Use these resources, and more, to ensure that your submission will be the best that you can make it.

Language (usage and editing services)
Please write your text in good English (American or British usage is accepted, but not a mixture of these). Authors who feel their English language manuscript may require editing to eliminate possible grammatical or spelling errors and to conform to correct scientific English may wish to use the English Language Editing service available from Elsevier's WebShop.

Submission

Submission to this journal proceeds totally online. Use the following guidelines to prepare your article. Via the homepage of this journal (http://www.ees.elsevier.com/phytochem) you will be guided stepwise through the creation and uploading of the various files. The system automatically converts source files to a single Adobe Acrobat PDF version of the article, which is used in the peer-review process. Please note that even though manuscript source files are converted to PDF at submission for the review process, these source files are needed for further processing after acceptance. All correspondence, including notification of the Editor's decision and requests for revision, takes place by e-mail and via the author's homepage, removing the need for a hard-copy paper trail.

Referees
Please submit, with the manuscript, the names, addresses and e-mail addresses of 4 potential referees. Note that the editor retains the sole right to decide whether or not the suggested reviewers are used.

Additional Information

Please submit regular articles to the appropriate Regional Editor for your geographical region. The address of the corresponding author defines which Regional Editor to select.


For the Americas and East Asia: Professor N. G. Lewis.
For the Rest of the World: Dr Richard J Robins.

  • Regular articles go to the appropriate geographical editor.
  • MOIs go to Dr Richard Robins.
  • Review articles should be pre-arranged with one the appropriate Regional Editor.
  • Special Issue Papers go to the Organizing Editor/Editors.
  • Solicited/Commissioned Reviews go to the editor who commissioned them.

Use of word processing software
It is important that the file be saved in the native format of the word processor used. The text should be in single-column format. Keep the layout of the text as simple as possible. Most formatting codes will be removed and replaced on processing the article. In particular, do not use the word processor's options to justify text or to hyphenate words. However, do use bold face, italics, subscripts, superscripts etc. When preparing tables, if you are using a table grid, use only one grid for each individual table and not a grid for each row. If no grid is used, use tabs, not spaces, to align columns. The electronic text should be prepared in a way very similar to that of conventional manuscripts (see also the Guide to Publishing with Elsevier). Note that source files of figures, tables and text graphics will be required whether or not you embed your figures in the text. See also the section on Electronic artwork.
To avoid unnecessary errors you are strongly advised to use the 'spell-check' and 'grammar-check' functions of your word processor.

Article Structure

The content of manuscripts must be arranged as follows: (1) a Graphical Abstract; (2) a Title Page with authors name(s) and address(es); (3) and Abstract, in which contents are briefly stated; (4) Keywords; (5) Introduction, and (6) the Results and Discussion (preferably combined). Although each section may be separated by headings, they should form one continuous narrative and only include details essential to the arguments presented. If a discussion is separately provided, it should not include a repetition of the results, but only indicate conclusions reached on the basis of them, and those from other referred works; (7) Conclusions or Concluding Remarks; (8) the Experimental should include brief details of the methods used such that a competent researcher in the field may be able to repeat the work; (9) Acknowledgments; (10) Figures and Legends, Formulae, Tables and References. Authors have to include pagination.

Subdivision - numbered sections
Divide your article into clearly defined and numbered sections. Subsections should be numbered 1.1. (then 1.1.1., 1.1.2., ...), 1.2., etc. (the abstract is not included in section numbering). Use this numbering also for internal cross-referencing: do not just refer to "the text". Any subsection may be given a brief heading. Each heading should appear on its own separate line.

Introduction

State the objectives of the work and provide an adequate background, avoiding a detailed literature survey or a summary of the results.

Specific names (genus, species, authority for the binomial) of all experimental plants must be given at first mention according to the Index Kewensis (searchable online at http://www.ipni.org) or similar authority (The Plant-Book: A Portable Dictionary of the Vascular Plants, by D.J. Mabberley, 2nd ed., June 1997, Cambridge University Press; ISBN: 0521414210), and preferably be in the form recommended by the International Code of Botanical Nomenclature. Named varieties of cultivars are given, e.g. Lactuca sativa cv. Grand Rapids. (The official printed version of the International Code of Botanical Nomenclature has been published as International Code of Botanical Nomenclature {Tokyo Code}. Regnum Vegetabile 131. Koeltz Scientific Books, Konigstein. ISBN 3-87429-367-X or 1-878762-66-4 or 80-901699-l-0.)

Theory/calculation
A Theory section should extend, not repeat, the background to the article already dealt with in the Introduction and lay the foundation for further work. In contrast, a Calculation section represents a practical development from a theoretical basis.

Results
Results should be clear and concise.

Discussion

This should explore the significance of the results of the work, not repeat them. A combined Results and Discussion section is often appropriate. Avoid extensive citations and discussion of published literature.

Experimental

Provide sufficient detail to allow the work to be reproduced. Methods already published should be indicated by a reference: only relevant modifications should be described.
Subsections on the Experimental Procedures should be italicized and inserted as part of the first line of the text to which they apply. Phytochemistry encourages an extensive use of abbreviations (these are listed at the back of the Instructions to Authors, or the reader is referred to other sources). The Experimental should begin with a subsection entitled General Experimental Procedures. This subsection will typically contain brief details of instruments used, and identification of sources of specialized chemicals, biochemicals and molecular biology kits.
The next subsection describes the source(s) and documentation of biological materials used, whether in reference to whole plants or parts therefrom, crude drugs, or any other plant material from which identifiable chemical substances are obtained for the first time. Documentation must also include a reference to voucher specimen(s) and voucher number(s) of the plants or other material examined. If available, authors should quote the name and address of the authority who identified each non-cultivated plant investigated. Specimens should preferentially be deposited in a major regional herbarium where the collection is maintained by state or private institution and which permits loan of such materials.
With other microorganisms, the culture collection from which they were either accessed and/or deposited should be included, together with identification of the strain designation code. The Experimental Procedures employed should be concise but sufficiently detailed that a qualified researcher will be able to repeat the studies undertaken, and these should emphasize either truly new procedures or essential modifications of existing procedures. Experimental details normally omitted include: (1) method of preparation of common chemical and biochemical derivatives, (2) excessive details of separation of compounds, proteins and enzymes, e.g. preparation of columns, TLC plates, column and fraction size.
Compound characterization: Physical and spectroscopic data for new compounds must be comprehensive, and follow the order shown below: compound name (and assigned number in text); physical state of compound (e.g. oil, crystal, liquid, etc.), melting and/or boiling point; optical rotation and/or circular dichroism measurements, if optically active; UV; IR, 1H NMR; 13C NMR; MS. For all new compounds, either high-resolution mass spectral or elemental analysis data are required.

Conclusions
The main conclusions of the study may be presented in a short Conclusions section, which may stand alone or form a subsection of a Discussion or Results and Discussion section.

Essential title page information
Title. Concise and informative. Titles are often used in information-retrieval systems. Avoid abbreviations and formulae where possible. "New" and "novel" are not allowed within title and abstract.
Author names and affiliations. Where the family name may be ambiguous (e.g., a double name), please indicate this clearly. Present the authors' affiliation addresses (where the actual work was done) below the names. Indicate all affiliations with a lower-case superscript letter immediately after the author's name and in front of the appropriate address. Provide the full postal address of each affiliation, including the country name, and, if available, the e-mail address of each author.
Corresponding author. Clearly indicate (marked by an asterisk) who will handle correspondence at all stages of refereeing and publication, also post-publication. Ensure that telephone and fax numbers (with country and area code) are provided in addition to the e-mail address and the complete postal address.
Present/permanent address. If an author has moved since the work described in the article was done, or was visiting at the time, a "Present address" (or "Permanent address") may be indicated as a footnote to that author's name. The address at which the author actually did the work must be retained as the main, affiliation address. Superscript Arabic numerals are used for such footnotes.

Abstract
A concise and factual abstract is required. The abstract should state briefly the purpose of the research, the principal results and major conclusions. An abstract is often presented separately from the article, so it must be able to stand alone. For this reason, References should be avoided, but if essential, then cite the author(s) and year(s). Also, non-standard or uncommon abbreviations should be avoided, but if essential they must be defined at their first mention in the abstract itself. The abstract should not contain compound numbers which refer to other parts of the manuscript, full chemical or known trivial names of compounds should be given.

Graphical abstract
Please provide, when submitting your article, a graphical abstract. This comprises the title, authors, identical to the article itself, a summary of about 25 words, and a pictogram: one figure representative of the work described. Maximum final dimensions of the pictogram are 5 x 5 cm: bear in mind readability after reduction, especially if using one of the figures from the article itself. Compound numbers can be given in the graphical abstract if they refer to a graphic also shown there. Graphical abstracts will be collated to provide a contents list for rapid scanning. For an example of recent graphical abstracts please download this pdf file. pdf link

Highlights
Highlights are mandatory for this journal. They consist of a short collection of bullet points that convey the core findings of the article and should be submitted in a separate editable file in the online submission system. Please use 'Highlights' in the file name and include 3 to 5 bullet points (maximum 85 characters, including spaces, per bullet point). You can view example Highlights on our information site.

Keywords

Authors must give 3-10 keywords or phrases, which identify the most important subjects covered by the paper. They should be placed at the beginning of the manuscript in the following order: name of plant species examined (Latin binomial); plant family; common epithet (where applicable); type of investigation; class of compound; protein or gene; name of compound(s); protein(s) and gene(s).

Chemical compounds
You can enrich your article by providing a list of chemical compounds studied in the article. The list of compounds will be used to extract relevant information from the NCBI PubChem Compound database and display it next to the online version of the article on ScienceDirect. You can include up to 10 names of chemical compounds in the article. For each compound, please provide the PubChem CID of the most relevant record as in the following example: Glutamic acid (PubChem CID:611). Please position the list of compounds immediately below the 'Keywords' section. It is strongly recommended to follow the exact text formatting as in the example below:
Chemical compounds studied in this article
Ethylene glycol (PubChem CID: 174); Plitidepsin (PubChem CID: 44152164); Benzalkonium chloride (PubChem CID: 15865)
More information.

Abbreviations

About, approximately: ca.
Anhydrous: dry (not anhyd.)
Aqueous: aq.
Circular dichroism: CD
Concentrated (or mineral acids): conc.
Concentrations: ppm (never ppb!), &mgr;M, mM, M, %
Dry weight: dry wt; fresh weight: fr. wt
Electricity: V, mA, eV
Force due to gravity (centrifugation): g; rpm (revolutions/min)
Gas chromatography: GC
Gas chromatography-mass spectrometry: GC-MS
trimethylsilyl derivative: TMSi (TMS cannot be used as this refers to the internal standard tetramethylsilane used in 1H NMR)
High performance liquid chromatography: HPLC
Infrared spectroscopy: IR
Length: nm, &mgr;m, mm, cm, m
Literature: lit.
Mass: pg, ng, &mgr;g, mg, g, kg
Mass spectrometry: m/z [M]+ (molecular ion, parent ion)
Melting points: uncorr. (uncorrected)
Molecular mass: Da (daltons), kDa
Molecular weight: Mr
Nuclear magnetic resonance: 1H NMR, 13C NMR, Hz, &dgr;
Numbers: e.g. 1, 10, 100, 1000, 10,000: per or -1
Optical rotatory dispersion: ORD
Paper chromatography: PC
Precipitate: ppt.
Preparative thin-layer chromatography: prep. TLC
Radioactivity: dpm (disintegrations per min), Ci (curie), sp. act (specific activity), Bq (1 becquerel = 1 nuclear transformation/sec)
Repetitive manipulations: once, twice, ×3, ×4, etc.
RRt (relative retention time), Rt (Kovat's retention index), ECL (equivalent chain length - term frequently used in fatty acid work)
Saturated: satd.
Solution: soln.
Solvent mixtures including chromatographic solvents: abbreviate as follows n-BuOH–HOAc–H2O (4:1:5)
Statistics: LSD (least significant difference), s.d. (standard deviation), s.e. (standard error)
Temperature: (with centigrade), mp, mps, mmp, bp
Temperature: temp.
Thin-layer chromatography: TLC, Rf
Time: s, min, h, day, week, month, year
Ultraviolet spectrophotometry: UV, A (absorbance, not OD - optical density)
Volume: l (litre), &mgr;l, ml
Weight: wt

For preparation of Inorganics and Organics please see the full instructions to authors, including all special characters, available for download as a pdf file. pdf link

For further terms used in biochemistry and molecular biology the authors should see the websites of the nomenclature committees. http://www.chem.qmul.ac.uk/iubmb/.

Acknowledgements
Collate acknowledgements in a separate section at the end of the article before the references and do not, therefore, include them on the title page, as a footnote to the title or otherwise. List here those individuals who provided help during the research (e.g., providing language help, writing assistance or proof reading the article, etc.).

Formatting of funding sources
List funding sources in this standard way to facilitate compliance to funder's requirements:

Funding: This work was supported by the National Institutes of Health [grant numbers xxxx, yyyy]; the Bill & Melinda Gates Foundation, Seattle, WA [grant number zzzz]; and the United States Institutes of Peace [grant number aaaa].

It is not necessary to include detailed descriptions on the program or type of grants and awards. When funding is from a block grant or other resources available to a university, college, or other research institution, submit the name of the institute or organization that provided the funding.

If no funding has been provided for the research, please include the following sentence:

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Nomenclature and Units

Chemical nomenclature, abbreviations and symbols must follow IUPAC rules. Whenever possible, avoid coining new trivial names; every effort should be made to modify an existing name. For example, when a new compound is described, it should be given a full systematic name according to IUPAC nomenclature and this should be cited in the Abstract or in the Experimental section. Isotopically-labeled substances should be written with the correct chemical name of the compound. The symbol for the isotope should be placed in square brackets and should precede that part of the name to which it refers, e.g. sodium [14C]formate.

In Table headings and legends on graph axes numerical data should be identified in the form data name/units.

For Presentation of Data please see the full instructions to authors, including all special characters, available for download as a pdf file. pdf link

Mass spectral data should be presented in full as Supplementary Information for all newly identified compounds. If the data are already published elsewhere then relevant references should be quoted. Presentation of mass spectral data should in general follow the recommendations given in Int. J. Mass Spectrom. Ion Processes, 142, 211-240 (1995), and must indicate the method used (EIMS, CIMS, GC-MS, TOFMS, FABMS, SIMS, APCI etc.) and the relevant experimental details (ionizing energy, voltages etc). The data should give only diagnostically important ions, the character of the fragmentation ions in relation to the molecular ion and the intensity relative to the major ion. For example-EIMS (probe) 70 eV, m/z (rel. int.): 386 [M]+ (36), 368 [M - H2O]+ (100), 353 [M - H2O - Me] + (23), 275 [M - 111] + (35), etc. CIMS (iso-butane, probe), 200 eV, m/z (rel. int.): 387 [M + H] + (100), 369 [(M + H) - H2O] + (23), etc. High-resolution spectra can be given in more detail if necessary for [M] + and the more important fragment ions.

X-ray crystallography
Only essential data (e.g. a three-dimensional structural drawing with bond distances) should be included in manuscripts. A complete list of data in CIF (Crystallographic Information File) format should be prepared separately and deposited with the Cambridge Crystallographic Data Centre (see http://www.ccdc.cam.ac.uk for further information) before the paper is submitted. A footnote indicating this fact is to be included in the manuscript. "CCDC...contains the supplementary crystallographic data for this paper. These data can be obtained free of charge via http://www.ccdc.cam.ac.uk/conts/retrieving.html (or from the CCDC, 12 Union Road, Cambridge CB2 1EZ, UK; fax: +44 1223 336033; e-mail: deposit@ccdc.cam.ac.uk)". Crystal structures of proteins should be submitted to the Protein Data Bank (see http://www.rcsb.org/pdb; e-mail: info@rcsb.org). Please submit a copy of the CIF data when you submit your manuscript.

Elemental analysis results for compounds which have been adequately described in the literature must be given in the form: (Found: C, 62.9; H, 5.4. Calc. for C13H13O4N: C, 63.2; H, 5.3%.) New compounds must be indicated by giving analytical results in the form: (Found: C, 62.9; H, 5.4. C13H13O4N requires: C, 63.2; H, 5.3%.)

Thin-layer chromatography
(a) For analytical TLC, dimensions of the plates can be deleted if layer thickness is 0.25 mm.
(b) Abbreviate common adsorbents: (but use silica gel, not SiO2 as this does not describe the material accurately), Al2O3 (alumina).
(c) Preparative forms of the technique should include details of (i) layer thickness (preparative TLC only), (ii) amount of sample applied to the layer, (iii) method of detection used to locate the bands and (iv) the solvent used to recover the compounds from the adsorbent after development.
(d) Special forms of TLC on impregnated adsorbents can be abbreviated, e.g. AgNO3-silica gel (1:9), by wt can be assumed.
(e) Solvent mixtures should be specified as under Abbreviations above.

Gas chromatography
(a) Detector used should be specified, e.g. dual FID, EC, etc.
(b) Carrier gas and flow rate or inlet pressure should be given, e.g. N2 at 3 ml min-1/10 psi.
(c) Operating conditions, such as injector and detector heater temperatures, oven temperature programme, should be included.
(d) Packed columns, e.g. 6 m x 3 mm (i.d. measurement only) packed with 1% SE-30 (support material and mesh size can be omitted unless unusual).
(e) Capillary columns the type (e.g. WCOT, SCOT), manufacturer's designation (e.g. DB5) and dimensions (length, internal/external diameter, film thickness) should be specified.

High performance liquid chromatography
(a) Solvent or solvent gradients used together with flow rate should be given.
(b) Column dimensions (length x i.d. only) and packing used.
(c) Method of detection employed, e.g. UV or refractive index.

Biochemical conventions
Unless a common biochemical term (e.g. ATP, NADH), biochemicals that are abbreviated should be spelled out in full (in brackets) immediately following their first usage in the text.

Enzyme names are typically not abbreviated, unless there are accepted abbreviations, such as ATPase. Where possible, E.C. numbers should be used for enzymes, and the recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB) should be used (see below).

Enzyme characterization
(a) Enzyme activity is expressed in units of katals (symbol kat), the conversion of one mol of substrate per sec. It should be made clear that the measurements were made under specified optimum conditions and were not seriously affected by losses during extraction and analysis.
(b) pH optima should be given together with pH values for half maximal activity.
(c) Kinetic parameters should be expressed as Vmax, Km etc.
(d) Enzyme inhibitors-effectiveness should be expressed as Ki or concentration for half-maximal activity.
(e) Optimal temperature of enzymes should not be given. This should be expressed in terms of "Energy of Activation" and "Energy of Activation for Denaturation".
(f) Enzyme nomenclature is now given in "Enzyme Nomenclature, Recommendations", Academic Press (1992) (http://www.chem.qmul.ac.uk/iubmb).
(g) Labeling of proteins and nucleic acids-use of labeled precursors in assessing the rate of synthesis of macromolecules must be validated by evidence of real, direct incorporation. The possibility of occlusion or adsorption of isotopic material should be noted and it should be shown that the labeled precursor is incorporated without prior catabolism.

Protein and nucleotide sequences
The Experimental must contain explicit documentation of the ends of nucleotide probes used in the study if previously unpublished, or by appropriate reference to published nucleotide numbers and/or restriction map. In manuscripts to be published in Phytochemistry, any new protein and/or nucleotide sequence must have been submitted to EMBL, GenBank™ or DNA Data Bank of Japan databases, with designated accession number(s) obtained prior to paper acceptance by the Regional Editor. The Author(s) must ensure access to this database information by timely release of data prior to publication, as well as providing necessary documentation to those already in the databases.


Nucleotide sequence data can be submitted either electronically (e-mail) or in computer-readable format, GenBank™, EMBL and the DNA Data Bank of Japan addresses are:
GenBank Submissions, National Center for Biotechnology Information, Building 38A, Room 8N-803, Bethesda, MD 20894. Tel.: +1 301 496-2475; e-mail (submissions): gb-sub@ncbi.nlm.nih.gov; e-mail (information): info@ncbi.nlm.nih.gov
EMBL Nucleotide Sequence Submissions, European Bioinformatics Institute, Hinxton Hall, Hinxton, Cambridge CB10 1SD, UK. Tel.: +44 (0) 1223-494401; fax: +44 (0) 1223-494472; e-mail: datasubs@ebi.ac.uk; world wide web: http://www.ebi.ac.uk/embl
DNA Data Bank of Japan, Center for Information Biology, National Institute of Genetics, Mishima, Shizuuoka 411-8540, Japan. Tel.: (+81) 559-81-6853; fax: (+81) 559-81-6849; e-mail: ddbjsub@ddbj.nig.ac.jp (for data submissions); world wide web: http://www.ddbj.nig.ac.jp.
Contributors must obtain the designated accession number, which will be incorporated into the paper, prior to printing.

Only novel DNA sequences will be published. Sequences that show close similarity to known coding or other sequences such as promoters will not be published and will be cited by accession number. Translated protein sequence information should be published as alignments against other gene family members. Papers containing such information about genes already known in other species should have sufficient novelty and biological significance. Sequence only papers or papers which duplicate work in another species will not be published.
Genes known by three letter names should be written in italics. The corresponding cognate protein should be written in capital, non-italic text.

Artwork

Electronic artwork
General points
• Make sure you use uniform lettering and sizing of your original artwork.
• Embed the used fonts if the application provides that option.
• Aim to use the following fonts in your illustrations: Arial, Courier, Times New Roman, Symbol, or use fonts that look similar.
• Number the illustrations according to their sequence in the text.
• Use a logical naming convention for your artwork files.
• Provide captions to illustrations separately.
• Size the illustrations close to the desired dimensions of the published version.
• Submit each illustration as a separate file.
A detailed guide on electronic artwork is available.
You are urged to visit this site; some excerpts from the detailed information are given here.
Formats
If your electronic artwork is created in a Microsoft Office application (Word, PowerPoint, Excel) then please supply 'as is' in the native document format.
Regardless of the application used other than Microsoft Office, when your electronic artwork is finalized, please 'Save as' or convert the images to one of the following formats (note the resolution requirements for line drawings, halftones, and line/halftone combinations given below):
EPS (or PDF): Vector drawings, embed all used fonts.
TIFF (or JPEG): Color or grayscale photographs (halftones), keep to a minimum of 300 dpi.
TIFF (or JPEG): Bitmapped (pure black & white pixels) line drawings, keep to a minimum of 1000 dpi.
TIFF (or JPEG): Combinations bitmapped line/half-tone (color or grayscale), keep to a minimum of 500 dpi.
Please do not:
• Supply files that are optimized for screen use (e.g., GIF, BMP, PICT, WPG); these typically have a low number of pixels and limited set of colors;
• Supply files that are too low in resolution;
• Submit graphics that are disproportionately large for the content.

Color Artwork

Colour in print: The charge for printed colour will be given on request by contacting authors@elsevier.com.
Colour on the web: Any figure can appear free of charge in colour in the web version of your article, regardless of whether or not this is reproduced in colour in the printed version. Please note that if you do not opt for colour in print, you should submit relevant figures in both colour (for the web) and black-and-white (for print).

Figure captions
Ensure that each illustration has a caption. Supply captions separately, not attached to the figure. A caption should comprise a brief title (not on the figure itself) and a description of the illustration. Keep text in the illustrations themselves to a minimum but explain all symbols and abbreviations used.

Tables

Please submit tables as editable text and not as images. Tables can be placed either next to the relevant text in the article, or on separate page(s) at the end. Number tables consecutively in accordance with their appearance in the text and place any table notes below the table body. Be sparing in the use of tables and ensure that the data presented in them do not duplicate results described elsewhere in the article. Please avoid using vertical rules.

References

Citation in text
Please ensure that every reference cited in the text is also present in the reference list (and vice versa). Any references cited in the abstract must be given in full. Unpublished results and personal communications are not recommended in the reference list, but may be mentioned in the text. Citation of a reference as "in press" implies that the item has been accepted for publication.

Reference management software
Most Elsevier journals have their reference template available in many of the most popular reference management software products. These include all products that support Citation Style Language styles, such as Mendeley and Zotero, as well as EndNote. Using the word processor plug-ins from these products, authors only need to select the appropriate journal template when preparing their article, after which citations and bibliographies will be automatically formatted in the journal's style. If no template is yet available for this journal, please follow the format of the sample references and citations as shown in this Guide.

Users of Mendeley Desktop can easily install the reference style for this journal by clicking the following link:
http://open.mendeley.com/use-citation-style/phytochemistry
When preparing your manuscript, you will then be able to select this style using the Mendeley plug-ins for Microsoft Word or LibreOffice.

Reference style
Text: All citations in the text should refer to:
1. Single author: the author's name (without initials, unless there is ambiguity) and the year of publication;
2. Two authors: both authors' names and the year of publication;
3. Three or more authors: first author's name followed by 'et al.' and the year of publication.
Citations may be made directly (or parenthetically). Groups of references should be listed first alphabetically, then chronologically.
Examples: 'as demonstrated (Allan, 2000a, 2000b, 1999; Allan and Jones, 1999). Kramer et al. (2010) have recently shown ....'
List: References should be arranged first alphabetically and then further sorted chronologically if necessary. More than one reference from the same author(s) in the same year must be identified by the letters 'a', 'b', 'c', etc., placed after the year of publication.
Examples:
Reference to a journal publication:
Van der Geer, J., Hanraads, J.A.J., Lupton, R.A., 2010. The art of writing a scientific article. J. Sci. Commun. 163, 51–59.
Reference to a book:
Strunk Jr., W., White, E.B., 2000. The Elements of Style, fourth ed. Longman, New York.
Reference to a chapter in an edited book:
Mettam, G.R., Adams, L.B., 2009. How to prepare an electronic version of your article, in: Jones, B.S., Smith , R.Z. (Eds.), Introduction to the Electronic Age. E-Publishing Inc., New York, pp. 281–304.
Reference to a website:
Cancer Research UK, 1975. Cancer statistics reports for the UK. http://www.cancerresearchuk.org/aboutcancer/statistics/cancerstatsreport/ (accessed 13.03.03).

Journal Abbreviations Source

Journal titles should be abbreviated (e.g. Carbohydr. Res.) following the Chemical Abstracts Service Source Index (CASSI) style (a list of abbreviated journal titles is available online at http://www.cas.org/sent.html).

Video

Elsevier accepts video material and animation sequences to support and enhance your scientific research. Authors who have video or animation files that they wish to submit with their article are strongly encouraged to include links to these within the body of the article. This can be done in the same way as a figure or table by referring to the video or animation content and noting in the body text where it should be placed. All submitted files should be properly labeled so that they directly relate to the video file's content. In order to ensure that your video or animation material is directly usable, please provide the files in one of our recommended file formats with a preferred maximum size of 150 MB. Video and animation files supplied will be published online in the electronic version of your article in Elsevier Web products, including ScienceDirect. Please supply 'stills' with your files: you can choose any frame from the video or animation or make a separate image. These will be used instead of standard icons and will personalize the link to your video data. For more detailed instructions please visit our video instruction pages. Note: since video and animation cannot be embedded in the print version of the journal, please provide text for both the electronic and the print version for the portions of the article that refer to this content.

Supplementary material

Supplementary material can support and enhance your scientific research. Supplementary files offer the author additional possibilities to publish supporting applications, high-resolution images, background datasets, sound clips and more. Please note that such items are published online exactly as they are submitted; there is no typesetting involved (supplementary data supplied as an Excel file or as a PowerPoint slide will appear as such online). Please submit the material together with the article and supply a concise and descriptive caption for each file. If you wish to make any changes to supplementary data during any stage of the process, then please make sure to provide an updated file, and do not annotate any corrections on a previous version. Please also make sure to switch off the 'Track Changes' option in any Microsoft Office files as these will appear in the published supplementary file(s). For more detailed instructions please visit our artwork instruction pages.

Database linking

Elsevier encourages authors to connect articles with external databases, giving readers access to relevant databases that help to build a better understanding of the described research. Please refer to relevant database identifiers using the following format in your article: Database: xxxx (e.g., TAIR: AT1G01020; CCDC: 734053; PDB: 1XFN). More information and a full list of supported databases.

CONTENT INNOVATION

AudioSlides

The journal encourages authors to create an AudioSlides presentation with their published article. AudioSlides are brief, webinar-style presentations that are shown next to the online article on ScienceDirect. This gives authors the opportunity to summarize their research in their own words and to help readers understand what the paper is about. More information and examples are available. Authors of this journal will automatically receive an invitation e-mail to create an AudioSlides presentation after acceptance of their paper.

Chemical Compound Viewer (Reaxys)

You can enrich your article with visual representations, links and details for those chemical structures that you define as the main chemical compounds described. Please follow the instructions to learn how to do this.

3D molecular models

You can enrich your online articles by providing 3D molecular models (optional) in PDB, PSE or MOL/MOL2 format, which will be visualized using the interactive viewer embedded within the article. Using the viewer, it will be possible to zoom into the model, rotate and pan the model, and change display settings. Submitted models will also be available for downloading from your online article on ScienceDirect. Each molecular model will have to be uploaded to the online submission system separately, via the '3D molecular models' submission category. More information.

Interactive plots

This journal enables you to show an Interactive Plot with your article by simply submitting a data file. Full instructions.

Submission checklist

The following list will be useful during the final checking of an article prior to sending it to the journal for review. Please consult this Guide for Authors for further details of any item.
Ensure that the following items are present:
One author has been designated as the corresponding author with contact details:
• E-mail address
• Full postal address
All necessary files have been uploaded, and contain:
• Keywords
• All figure captions
• All tables (including title, description, footnotes)
Further considerations
• Manuscript has been 'spell-checked' and 'grammar-checked'
• References are in the correct format for this journal
• All references mentioned in the Reference list are cited in the text, and vice versa
• Permission has been obtained for use of copyrighted material from other sources (including the Internet)
Printed version of figures (if applicable) in color or black-and-white
• Indicate clearly whether or not color or black-and-white in print is required.
For any further information please visit our Support Center.

Additional Information

Is the subject matter really appropriate to Phytochemistry?
Is the work described both new and significant?
Have you supplied a Graphical Abstract?
Is the Title both short and informative?
Does the Abstract fully represent your scientific contribution? Is it self-contained? (Avoid formulae, numbers and abbreviations given in the text.)
Have you avoided repeating yourself? Have you avoided presenting the same data more than once? Can you really justify writing separate 'Results' and 'Discussion' sections?
Have you checked plant names? Are you sure of the identity of the plants examined? Have you indicated the part of the plant you extracted? Have you deposited a voucher specimen and given access information?
Have you remembered to add the accepted IUPAC systematic names for new plant products?
Have you used all the suggested abbreviations in the Experimental?
Have you remembered to enclose with (or cite in) your manuscript and other relevant papers (e.g. reprint of previous paper in a series, any manuscripts of papers in press referred to in the paper, etc.)?
Is your manuscript double-spaced throughout with adequate margins and consists of one file containing all your text, figures and tables with a file name extension, plus separate original graphic files ready for online submission?

Online proof correction

Corresponding authors will receive an e-mail with a link to our online proofing system, allowing annotation and correction of proofs online. The environment is similar to MS Word: in addition to editing text, you can also comment on figures/tables and answer questions from the Copy Editor. Web-based proofing provides a faster and less error-prone process by allowing you to directly type your corrections, eliminating the potential introduction of errors.
If preferred, you can still choose to annotate and upload your edits on the PDF version. All instructions for proofing will be given in the e-mail we send to authors, including alternative methods to the online version and PDF.
We will do everything possible to get your article published quickly and accurately. Please use this proof only for checking the typesetting, editing, completeness and correctness of the text, tables and figures. Significant changes to the article as accepted for publication will only be considered at this stage with permission from the Editor. It is important to ensure that all corrections are sent back to us in one communication. Please check carefully before replying, as inclusion of any subsequent corrections cannot be guaranteed. Proofreading is solely your responsibility.

Offprints

The corresponding author will, at no cost, receive a customized Share Link providing 50 days free access to the final published version of the article on ScienceDirect. The Share Link can be used for sharing the article via any communication channel, including email and social media. For an extra charge, paper offprints can be ordered via the offprint order form which is sent once the article is accepted for publication. Both corresponding and co-authors may order offprints at any time via Elsevier's Webshop. Corresponding authors who have published their article open access do not receive a Share Link as their final published version of the article is available open access on ScienceDirect and can be shared through the article DOI link.



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