Zone Electrophoresis - 2nd Edition - ISBN: 9781483197067, 9781483222097

Zone Electrophoresis

2nd Edition

Chromatographic and Electrophoretic Techniques

Editors: Ivor Smith
eBook ISBN: 9781483222097
Imprint: Butterworth-Heinemann
Published Date: 1st January 1968
Page Count: 534
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Description

Chromatographic and Electrophoretic Techniques, Volume II: Zone Electrophoresis, Second Edition presents tried and tested methods of zone electrophoresis, which has been carried out on commercially available apparatus offering many advantages to the majority of laboratories. This book is divided into 14 chapters and begins with a brief introduction to the general principles of zone electrophoresis. The subsequent chapters deal with the principles, instrumentation, and applications of various methods of zone electrophoresis, including low- and high-voltage paper, cellulose-acetate, thin-layer, agar- and starch-gel, and preparative block electrophoresis. The remaining chapters cover other zone electrophoresis methods, such as acrylamide gel, disc, acrylamide flat gel, and continuous electrophoresis. This book is of value to analytical chemists and biologists.

Table of Contents


Editors and Authors

Preface to Second Edition

Preface

1. General Principles of zone Electrophoresis

2. Paper Electrophoresis at Low Voltage

Techniques of Paper Electrophoresis

The Separation of Serum Proteins, Lipoproteins, Glycoproteins, Phosphate, Cholesterol

Clinical Interpretations of Serum Electrophoretic Patterns

Hemoglobins

Carbohydrate-Protein Compounds and Acid Glycosaminoglycans

One Way Separation of Aminoacids

Two Way Screening Procedures for Aminoacids, Indoles and Imidazoles

3. Cellulose Acetate Electrophoresis and Immunodiffusion Techniques

4. Thin-Layer Electrophoresis

5. High Voltage Paper Electrophoresis

6. Agar Gel Electrophoresis and Immuno-Electrophoresis

7. Preparative Block Electrophoresis

8. Starch Gel Electrophoresis

Techniques of Starch Gel Electrophoresis

Micro Starch Gel Electrophoresis

Application to the Study of Serum and Urine Proteins

Hemoglobins

9. Starch Gel Zone Electrophoresis of Enzymes

10. Disc Electrophoresis on Acrylamide Gels

Techniques of Disc Electrophoresis

Protein Patterns in Human Serum; Interpretation

Separation of Milk Proteins

C.S.F. Electrophoresis

11. Disc Electrophoresis of RNA in Polyacrylamide Gels

Electrophoresis of RNA

The Fractionation of High Molecular Weight RNA

12. Acrylamide Flat Slab Gel Electrophoresis

Horizontal Gel Slab Electrophoresis

The Relationship between Mobility, Gel Concentration and Molecular Weight

Micro Gel Electrophoresis

Vertical Flat Bed Gel Electrophoresis

13. Preparative Acrylamide Disc Electrophoresis

Section 1.

Section 2.

14. Continuous Electrophoresis

Index


Details

No. of pages:
534
Language:
English
Copyright:
© Butterworth-Heinemann 1968
Published:
Imprint:
Butterworth-Heinemann
eBook ISBN:
9781483222097

About the Editor

Ivor Smith