Zone Electrophoresis

Zone Electrophoresis

Chromatographic and Electrophoretic Techniques

2nd Edition - January 1, 1968

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  • Editor: Ivor Smith
  • eBook ISBN: 9781483222097

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Description

Chromatographic and Electrophoretic Techniques, Volume II: Zone Electrophoresis, Second Edition presents tried and tested methods of zone electrophoresis, which has been carried out on commercially available apparatus offering many advantages to the majority of laboratories. This book is divided into 14 chapters and begins with a brief introduction to the general principles of zone electrophoresis. The subsequent chapters deal with the principles, instrumentation, and applications of various methods of zone electrophoresis, including low- and high-voltage paper, cellulose-acetate, thin-layer, agar- and starch-gel, and preparative block electrophoresis. The remaining chapters cover other zone electrophoresis methods, such as acrylamide gel, disc, acrylamide flat gel, and continuous electrophoresis. This book is of value to analytical chemists and biologists.

Table of Contents


  • Editors and Authors

    Preface to Second Edition

    Preface

    1. General Principles of zone Electrophoresis

    2. Paper Electrophoresis at Low Voltage

    Techniques of Paper Electrophoresis

    The Separation of Serum Proteins, Lipoproteins, Glycoproteins, Phosphate, Cholesterol

    Clinical Interpretations of Serum Electrophoretic Patterns

    Hemoglobins

    Carbohydrate-Protein Compounds and Acid Glycosaminoglycans

    One Way Separation of Aminoacids

    Two Way Screening Procedures for Aminoacids, Indoles and Imidazoles

    3. Cellulose Acetate Electrophoresis and Immunodiffusion Techniques

    4. Thin-Layer Electrophoresis

    5. High Voltage Paper Electrophoresis

    6. Agar Gel Electrophoresis and Immuno-Electrophoresis

    7. Preparative Block Electrophoresis

    8. Starch Gel Electrophoresis

    Techniques of Starch Gel Electrophoresis

    Micro Starch Gel Electrophoresis

    Application to the Study of Serum and Urine Proteins

    Hemoglobins

    9. Starch Gel Zone Electrophoresis of Enzymes

    10. Disc Electrophoresis on Acrylamide Gels

    Techniques of Disc Electrophoresis

    Protein Patterns in Human Serum; Interpretation

    Separation of Milk Proteins

    C.S.F. Electrophoresis

    11. Disc Electrophoresis of RNA in Polyacrylamide Gels

    Electrophoresis of RNA

    The Fractionation of High Molecular Weight RNA

    12. Acrylamide Flat Slab Gel Electrophoresis

    Horizontal Gel Slab Electrophoresis

    The Relationship between Mobility, Gel Concentration and Molecular Weight

    Micro Gel Electrophoresis

    Vertical Flat Bed Gel Electrophoresis

    13. Preparative Acrylamide Disc Electrophoresis

    Section 1.

    Section 2.

    14. Continuous Electrophoresis

    Index


Product details

  • No. of pages: 534
  • Language: English
  • Copyright: © Butterworth-Heinemann 1968
  • Published: January 1, 1968
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483222097

About the Editor

Ivor Smith

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