The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* This volume and its companion Volumes 279, 280, and 281 provide * A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes * Details on physical, chemical, and biological properties of vitamins and coenzymes * Chemical and biological syntheses of vitamins, coenzymes, and their analogs * Aspects of transport and metabolism of vitamins and coenzymes


Biochemists, nutritionists, cell biologists, pharmacologists, molecular biologists, and physiologists.

Table of Contents

Vitamin A: A.W. Norris and E. Li, Generation and Characterization of Cellular Retinoic Acid-Binding Proteins from Escherichia coli Expression System. M. Clagett-Dame and J.J. Repa, Generating and Characterizing Retinoid Receptors from Escherichia coli and Insect Cell Expression Systems. E.A. Allegretto and R.A. Heyman, Expression and Characterization of Retinoid Receptors in Yeast. A. Rowe and P.M. Brickell, Use of in Situ Hybridization Techniques to Study Embryonic Expression of Retinoid Receptors and Binding Proteins. N. Ferrari, G. Vidali, and U. Pfeffer, Use of Quantitative PCR to Study Retinoid Receptor Expression. L. Zhou, G. Otulakowski, and C.Y. Lau, Use of Quantitative PCR to Study Cellular Retinoic Acid Binding Protein-II mRNA Expression in Human Skin. L. Wojnowski and A. Zimmer, Use of Transgenic Mice to Study Activation of Retinoic Acid-Responsive Promoters. M. Saitou, T. Tanaka, and A. Kakizuka, Use of Transgenic Mice to Eliminate Retinoic Acid Receptor Function in Specific Tissues. M. Wagner, Use of Reporter Cells to Study Endogenous Retinoid Sources in Embryonic Tissues. P.K. Tadikonda and H.F. DeLuca, Preparation of Radiolabeled9-cis- and all-trans-Retinoids. X-D. Wang and N.I. Krinsky, Identification and Quantification of Retinoic Acid and Other Metabolites from (-Carotene Excentric Cleavage in Human Intestine in Vitro and Ferret Intestine in Vivo. R.S. Parker, J.T. Brenna, J.E. Swanson, K.J. Goodman, and B. Marmor, Assessing Metabolism of (-[13C] Carotene Using High Precision Isotope Ratio Mass Spectrometry. G. Tang, B.A. Andrien, G.G. Donikowski, and R.M. Russell, Atmospheric Pressure Chemi


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© 1997
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY