The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* This volume and its companion Volumes 279, 281, and 282 provide * A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes * Details on physical, chemical, and biological properties of vitamins and coenzymes * Chemical and biological syntheses of vitamins, coenzymes, and their analogs * Aspects of transport and metabolism of vitamins and coenzymes


Biochemists, nutritionists, cell biologists, pharmacologists, molecular biologists, and physiologists

Table of Contents

Pyridoxine, Pyridoxamine, Pyridoxal: Analogs and Derivatives: H. Tsuge, Determination of Vitamin B 6 Vitamers and Metabolites in Biological Sample. H.J. Mascher, High-Performance Liquid Chromatography Determination of Total Pyridoxal in Human Plasma. J.D. Mahuren and S.P. Coburn, Determination of 5-Pyridoxic Acid, 5-Pyridoxic Acid Lactone, and other Vitamin B6 Compounds by Cation-Exchange High-Performance Liquid Chromatography. D.E. Metzler, Nuclear Magnetic Resonance in Study of Active Sites of Pyridoxal-Dependent Enzymes. T. Fukui and K. Tanizawa, Synthesis and Application of Pyridoxal Polyphosphoryl Derivatives as Active-Site Probes for Nucleotide-Binding Enzymes. S. Stein and T. Zhu, Preparation of Vitamin B6-Peptide and Vitamin B6-Peptide-Oligonucleotide Conjugates. J.F. Gregory III and H. Nakano, Preparation of Nonlabeled, Tritiated, and Deuterated Pyridoxine 5-beta-D-Glucoside and Assay of Pyridoxine-5-beta-D-Glucoside Hydrolase. Y. Suzuki, Y. Doi, K. Uchida, and H. Tsuge, Enzymatic Preparation of Pyridoxine 4- and 5-alpha-D-Glucosides. Y. Suzuki and K. Uchida, Formation of Beta-Galactosides of Pyridoxine using Sporobolomyces singularis. Carbonyl Coenzymes: Pyruvyl Enzymes and Quinoproteins: W. Dowhan, Phosphatidylserine Decarboxylases: Pyruvoyl-Dependent Enzymes from Bacteria to Mammals. M. Misset-Smits, A.J.J. Olshoorn, A. Dewanti, and J.A. Duine, Production, Assay, and Occurrence of Pyrroloquinoline Quinone. C. Hartmann and W.S. McIntire, Amine-Oxidizing Quinoproteins. O. Suzuki and T. Kumazawa, Gas Chromatographic/Mass Spectrometric Analysis of Pyrroloquinoline Quinone. H. Narita and E. Morishita,


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© 1997
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY