This volume of Methods in Enzymology and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before.
- This volume and its companion Volumes 280, 281, and 282 provide
- A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes
- Details on physical, chemical, and biological properties of vitamins and coenzymes
- Chemical and biological syntheses of vitamins, coenzymes, and their analogs
- Aspects of transport and metabolism of vitamins and coenzymes
Biochemists, Nutritionists, Cell Biologists, Pharmacologists, Molecular Biologists, and Physiologists
E. Kimoto, S. Terada, and T. Yamaguchi, Analysis of Ascorbic Acid, Dehydroascorbic Acid, and Transformation Products by Ion-Pairing High Performance Liquid Chromatography with Multiwavelength Ultraviolet and Electrochemical Detection.
J.C. Deutsch, Gas Chromatographic/Mass Spectrometric Measurements of Ascorbic Acid and Analysis of Ascorbic Acid Degradation in Solution.
K. Yagi and M. Nishiskimi, Expression of Recombinant L-Gulono-gamma-lactone Oxidase.
E. Maellaro, B. Del Bello, L. Sugherini, M. Comporti, and A.F. Casini, Purification and Characterization of Gluathione-Dependent Dehydroascorbic Reductase from Rat Liver.
A.S. Kolhekar, R.E. Mains, and B.A. Eipper, Peptidylglycine alpha-Amidating Monooxygenase: An Ascorbate-Requiring Enzyme.
M. Levine, S. Rumsey, and Y. Wang, Principles Involved in Formulating Recommendations for Vitamin C Intake: A Paradigm for Water-Soluble Vitamins.
Thiamin: Phosphates and Analogs:
V. Fayol, High-Performance Liquid Chromatography Determination of Total Thiamin in Biological and Food Products.
C.M.E. Tallaksen, T. Bohmer, J. Karlsen, and H. Bell, Determination of Thiamin and Its Phosphate Esters in Human Blood, Plasma, and Urine.
J. Gerrits, H. Eidhof, J.W.I. Brunnekreeft, and J. Hessels, Determination of Thiamin and Thiamin Phosphates in Whole Blood by Reversed-Phase Liquid Chromatography with Precolumn Derivtization.
H.J. Mascher and C. Kikuta, High Performance Liquid Chromatography Determination of Total Thiamin in Human Plasma.
D.T. Wyatt and R.E. Hillman, Cyanogen Bromide-Based Assay of Thiamin.
K. Tazuya, K. Yamada, and H. Kumaoka, Isotopically Labled Precursors and Mass Spectrometry in Elucidating Biosynt
- No. of pages:
- © Academic Press 1997
- 16th June 1997
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
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