Description

This volume of Methods in Enzymology and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before.

Key Features

  • This volume and its companion Volumes 280, 281, and 282 provide
  • A collation of the most recent and useful methods for the identification, preparation, and quantification of vitamins and coenzymes
  • Details on physical, chemical, and biological properties of vitamins and coenzymes
  • Chemical and biological syntheses of vitamins, coenzymes, and their analogs
  • Aspects of transport and metabolism of vitamins and coenzymes

Readership

Biochemists, Nutritionists, Cell Biologists, Pharmacologists, Molecular Biologists, and Physiologists

Table of Contents

Ascorbic Acid: E. Kimoto, S. Terada, and T. Yamaguchi, Analysis of Ascorbic Acid, Dehydroascorbic Acid, and Transformation Products by Ion-Pairing High Performance Liquid Chromatography with Multiwavelength Ultraviolet and Electrochemical Detection. J.C. Deutsch, Gas Chromatographic/Mass Spectrometric Measurements of Ascorbic Acid and Analysis of Ascorbic Acid Degradation in Solution. K. Yagi and M. Nishiskimi, Expression of Recombinant L-Gulono-gamma-lactone Oxidase. E. Maellaro, B. Del Bello, L. Sugherini, M. Comporti, and A.F. Casini, Purification and Characterization of Gluathione-Dependent Dehydroascorbic Reductase from Rat Liver. A.S. Kolhekar, R.E. Mains, and B.A. Eipper, Peptidylglycine alpha-Amidating Monooxygenase: An Ascorbate-Requiring Enzyme. M. Levine, S. Rumsey, and Y. Wang, Principles Involved in Formulating Recommendations for Vitamin C Intake: A Paradigm for Water-Soluble Vitamins. Thiamin: Phosphates and Analogs: V. Fayol, High-Performance Liquid Chromatography Determination of Total Thiamin in Biological and Food Products. C.M.E. Tallaksen, T. Bohmer, J. Karlsen, and H. Bell, Determination of Thiamin and Its Phosphate Esters in Human Blood, Plasma, and Urine. J. Gerrits, H. Eidhof, J.W.I. Brunnekreeft, and J. Hessels, Determination of Thiamin and Thiamin Phosphates in Whole Blood by Reversed-Phase Liquid Chromatography with Precolumn Derivtization. H.J. Mascher and C. Kikuta, High Performance Liquid Chromatography Determination of Total Thiamin in Human Plasma. D.T. Wyatt and R.E. Hillman, Cyanogen Bromide-Based Assay of Thiamin. K. Tazuya, K. Yamada, and H. Kumaoka, Isotopically Labled Precursors and Mass Spectrometry in Elucidating Biosynt

Details

No. of pages:
502
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
Print ISBN:
9780121821807
Electronic ISBN:
9780080883915

Reviews

Praise for the Series:
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE
"Incomparably useful." --ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY