Description

The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work!

Key Features

@introbul:Key Features @bul:* Provides up-to-date techniques by experts worldwide * Presents common, step-by-step protocols in an attractive, easy-to-use fashion * Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory

Readership

Research scientists and research clinicians working in the field of virology will find this book useful. It will also be of interest to molecular biologists and immunologists, to the infectious disease community in general, andto biotechnology/pharmaceutical companies.

Table of Contents

Classical Techniques: V.C. George, J.C. Hierholzer, and E.W. Ades, Cell Culture. J.C. Hierholzer and R.A. Killington, Virus Isolation and Quantitation. J.C. Hierholzer, R.A. Killington, and A. Stokes, Preparation of Antigens. R.A. Killington, A. Stokes, and J.C. Hierholzer, Virus Purification. I.L. Chrystie, Electron Microscopy. M. Chernesky, Traditional and Serological Tests. M. Chernesky and J. Mahoney, Immunoassays. A. Mawle, Cell Mediated Immunity. Techniques in Molecular Virology: J.E. Novak, T.C. Jarvis, and K. Kirkegaard, RNA: Transcription, Transfection, and Quantitation. D.J. McCance, DNA Viruses: DNA Extraction, Purification, and Characterization. K.N. Leppard and C.R. Pringle, Virus Mutants. D.R. Harper and B.F. Coles, Polypeptides. M. McCrae, The Assay of Viral Enzyme. Medical Virology: A.L. Jabri, D. Wigg, and J.S. Oxford, Initial In vitro Screening of DrugCandidates for their Potential Antiviral Activities. S. Specter and D. Jeffries, Detection of Virus and Viral Antigens. J. Mahoney, Detection of Viral Nucleic Acids in Clinical Material. S. Specter and D. Jeffries, Serological Methods. S. Specter and D. Jeffries, Disinfection. Appendices. Subject Index.

Details

No. of pages:
374
Language:
English
Copyright:
© 1996
Published:
Imprint:
Academic Press
Print ISBN:
9780124653306
Electronic ISBN:
9780080543581

About the authors

Hillar Kangro

Affiliations and Expertise

SmithKlein Beecham Biologicals S.A., Rixensart, Belgium

Brian Mahy

Affiliations and Expertise

Coordinating Center for Infectious Diseases, CDC, Atlanta, GA, USA