Description

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Expression, purification, and characterization * Activity assays * Kinetic and screening * Design and analysis of substrates and inhibitors * Molecular and structural characterizations

Readership

Biochemists, molecular biologists, virologists, medicinal chemists, immunologists, and graduate students in these disciplines; drug company researchers.

Table of Contents

Expression, Purification, and Characterization: V. Sardana and M. Sardana, Purification of Viral Polymerases: General Considerations. P.E. Boehmer, Expression, Purification, and Characterization of the HerpesSimplex Virus Type-1 DNA Polymerase. D.J. Barton, B.J. Morasco, and J.B. Flanegan, Assays for Poliovirus Polymerase, 3Dpol, and Authentic RNA Replication in HeLa S10 Extracts. R. de Francesco, S.-E. Behrens, L. Tomei, S. Altamura, and J. Jiricny, RNA-Dependent RNA Polymerase of Hepatitus C Virus. J. Herold, S. Siddell, and J. Ziebuhr, Characterization of Coronavirus RNA Polymerase Gene Products. J. Tomassini, Expression, Purification, and Characterization of Orthomyxovirus: Influenza Transcriptase. T. Das, B.P. De, and A.K. Banerjee, Expression, Purification, and Characterization of Rhabdovirus Polymerase. M.W. Stahlhut and D.B. Olsen, Expression and Purification of Retroviral HIV-1 Reverse Transcriptases. L.H.Malkas and R.J. Hickey, Expression, Purification, and Characterization of DNA Polymerases Involved in Papovavirus Replication. M. Ramachandra, Y. Sasaguri, R. Nakano, and R. Padmanabhan, Heterologous Expression, Purification, and Characterization of Adenovirus DNA Polymerase and Preterminal Protein. J. Hu and C. Seeger, Expression and Characterization of Hepadnavirus Reverse Transcriptases. P. Gershon and B. Moss, Expression, Purification, and Characterization of Vaccinia Virus Encoded RNA and Poly(A)Polymerases. J.R. Sayers, Viral Polymerase-Associated 5-3 Exonucleases: Expression, Purification, and Uses. Activity Assays: Kinetic and Screening: R.D. Kuchta, Isotopic Assays of Viral Polymerases and Related Proteins.J. Eberle and C. W. Knopf, Nonisotopic Assays of Viral Polymerases and Related Proteins. S. Gabbara and J.A. Peliska, Catalytic Activities Associated with Retrov

Details

No. of pages:
658
Language:
English
Copyright:
© 1996
Published:
Imprint:
Academic Press
Print ISBN:
9780121821760
Electronic ISBN:
9780080883885

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY