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Viral Polymerases and Related Proteins - 1st Edition - ISBN: 9780121821760, 9780080883885

Viral Polymerases and Related Proteins, Volume 275

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Lawrence Kuo David Olsen Steven Carroll
Hardcover ISBN: 9780121821760
eBook ISBN: 9780080883885
Imprint: Academic Press
Published Date: 24th September 1996
Page Count: 658


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Table of Contents

Expression, Purification, and Characterization: V. Sardana and M. Sardana, Purification of Viral Polymerases: General Considerations. P.E. Boehmer, Expression, Purification, and Characterization of the HerpesSimplex Virus Type-1 DNA Polymerase. D.J. Barton, B.J. Morasco, and J.B. Flanegan, Assays for Poliovirus Polymerase, 3Dpol, and Authentic RNA Replication in HeLa S10 Extracts. R. de Francesco, S.-E. Behrens, L. Tomei, S. Altamura, and J. Jiricny, RNA-Dependent RNA Polymerase of Hepatitus C Virus. J. Herold, S. Siddell, and J. Ziebuhr, Characterization of Coronavirus RNA Polymerase Gene Products. J. Tomassini, Expression, Purification, and Characterization of Orthomyxovirus: Influenza Transcriptase. T. Das, B.P. De, and A.K. Banerjee, Expression, Purification, and Characterization of Rhabdovirus Polymerase. M.W. Stahlhut and D.B. Olsen, Expression and Purification of Retroviral HIV-1 Reverse Transcriptases. L.H.Malkas and R.J. Hickey, Expression, Purification, and Characterization of DNA Polymerases Involved in Papovavirus Replication. M. Ramachandra, Y. Sasaguri, R. Nakano, and R. Padmanabhan, Heterologous Expression, Purification, and Characterization of Adenovirus DNA Polymerase and Preterminal Protein. J. Hu and C. Seeger, Expression and Characterization of Hepadnavirus Reverse Transcriptases. P. Gershon and B. Moss, Expression, Purification, and Characterization of Vaccinia Virus Encoded RNA and Poly(A)Polymerases. J.R. Sayers, Viral Polymerase-Associated 5-3 Exonucleases: Expression, Purification, and Uses. Activity Assays: Kinetic and Screening: R.D. Kuchta, Isotopic Assays of Viral Polymerases and Related Proteins.J. Eberle and C. W. Knopf, Nonisotopic Assays of Viral Polymerases and Related Proteins. S. Gabbara and J.A. Peliska, Catalytic Activities Associated with Retroviral and Viral Polymerases. J.L. Cole, Approaches to High Volume ScreeningAssays of Viral Polymerases and Related Proteins. S.M. Oberhaus and J.E. Newbold, In Situ DNA Polymerase and RNase H Activity Gel Assays as Applied to Hepadnavirus Particles. V. Byrnes and D. Hazuda, A System to Analyze and Identify Inhibitorsof HIV-1 Gene Regulation with Defective Integrated Provirus. Design and Analysis of Substrates and Inhibitors: S.S. Carroll, F. Benseler, and D.B. Olsen, Preparation and Use of Synthetic Oligoribonucleotides as Tools for Study of Viral Polymerases. V.V. Gurevich, Use of Bacteriophage RNA Polymerase in RNA Synthesis. J.E. Wilson, D.J.T. Porter, and J.E. Reardon, Inhibition of Viral Polymerases by Chain Terminating Substrates: A Kinetic Analysis. E.J. Prisbe and M.S. Chen, Design of Nucleoside Analogue Inhibitors of Herpesvirus Polymerases. T.J. Tucker, W.C. Lumma, and J.C. Culberson, Development of Nonnucleoside HIV Reverse Transcriptase Inhibitors. J. Balzarini and E. De Clerq, Analysis of Inhibition of Retroviral Reverse Transcriptase. H. Chen, D. Brown, and L. Gold, Novel Methods of Generating Specific Oligonucleotide Inhibitors of Viral Polymerases. Molecular and Structural Characterizations: J.C. Boyer, K. Bebenek, and T.A. Kunkel, AnalyzingFidelity of Transcription and Reverse Transcription. P.L. Boyer and S.H. Hughes, Site-Directed Mutagenic Analysis of Viral Polymerases and Related Proteins. J.S. Oxford, A.A. Al-Jabri, and P. Levantis, Analysis of Resistance Mutants of ViralPolymerases. Author Index. Subject Index.


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Expression, purification, and characterization

  • Activity assays
  • Kinetic and screening
  • Design and analysis of substrates and inhibitors
  • Molecular and structural characterizations


Biochemists, molecular biologists, virologists, medicinal chemists, immunologists, and graduate students in these disciplines; drug company researchers.


No. of pages:
© Academic Press 1996
24th September 1996
Academic Press
Hardcover ISBN:
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Lawrence Kuo

Affiliations and Expertise

Merck Sharp & Dohme Research Laboratories, Merck & Co., West Point, Pennsylvania, U.S.A.

David Olsen

Affiliations and Expertise

Merck Sharp & Dohme Research Laboratories, Merck & Co., Inc., West Point, Pennsylvania, U.S.A.

Steven Carroll

Affiliations and Expertise

Merck Sharp & Dohme Research Laboratories, Merck & Co., Inc., West Point, Pennsylvania, U.S.A.