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Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies.
Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.
- Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the field
- Emphasizes in vivo, in situ and non-invasive analysis of proteins in biomedical and life science research more broadly
- Contains chapters that address vibrational spectroscopy for the study of simple purified proteins and protein aggregates, fibrous proteins, membrane proteins and protein assemblies
Active researchers and students in biochemistry, molecular biology, pharmaceutical science, cellular biology, applied microbiology, and biotechnology; medical chemists; clinician scientists
1. ATR-FTIR Spectroscopy and Spectroscopic Imaging of Proteins
Bernadette Byrne, James W. Beattie, Cai Li Song and Sergei G. Kazarian
2. Light-Induced Difference FTIR Spectroscopy of Photoreceptive Proteins
Hideki Kandori and Kota Katayama
3. Quantum Cascade Laser-based IR Transmission Spectroscopy of Proteins in Solution
Andreas Schwaighofer and Bernhard Lendl
4. Theoretical Simulation of Protein Two-Dimentional Infrared Spectroscopy
Zhijun Pan, Xian Chen, Tan Jin, Lu Zhang and Wei Zhuang
5. Infrared Spectroscopy and Imaging for Understanding Neurodegenerative Protein-Folding Diseases
Lisa M. Miller
6. Near-infrared (NIR) spectroscopy in protein research
Mika Ishigaki and Yukihiro Ozaki
7. Vibrational imaging of proteins: changes in the tissues and cells in the lifestyle disease studies
Katarzyna Majzner, Krzysztof Czamara, Marta Z. Pacia, Jakub Dybas, Ewelina Bik, Karolina Chrabaszcz, Ewelina Wiercigroch, Aleksandra Dorosz, Adrianna Wislocka, Katarzyna M. Marzec, Kamilla Malek and Malgorzata Baranska
8. Interpretation of Vibrational Optical Activity Spectra of Proteins
Jana Hudecová and Petr Bouř
9. Nano-scale analysis of protein self-assemblies
10. Vibrational Spectroscopic Analysis and Quantification of Proteins in Human Blood Plasma and Serum
Clement Bruno, James M. Cameron, Drishya Rajan Parachalil, Matthew J. Baker, Franck Bonnier, Holly J. Butler and Hugh J. Byrne
11. Vibrational spectroscopy in protein research towards virus identification: challenges, new research and future perspectives
Marfran C. D. Santos, Camilo L. M. Morais, Kássio M. G. Lima and Francis L. Martin
12. Two-Dimensional Correlation Spectroscopy of Proteins
Yeonju Park, Isao Noda, Young Mee Jung
13. Resonance Raman spectroscopy of hemoglobin in red blood cells
Bayden R. Wood, Kamila Kochan and Katarzyna M. Marzec
14. Ultraviolet Raman spectroscopy for understanding structure and formation mechanism of amyloid fibrils
Joseph Handen and Igor K. Lednev
15. Recent advances in Raman spectroscopy of proteins for disease diagnosis
Keita Iwasaki, Hemanth Noothalapati, and Tatsuyuki Yamamoto
16. Dynamics and allostery of human hemoglobin as elucidated by time-resolved resonance Raman spectroscopy
17. Immuno-SERS: from nanotag design to assays and microscopy
Yuling Wang, Yuying Zhang and Sebastian Schlücker
18. Highly localized characterization of protein structure and interaction by surface-enhanced Raman scattering
19. Surface-enhanced Raman scattering (SERS) chemosensing of proteins
Luca Guerrini and Ramon A. Alvarez-Puebla
- No. of pages:
- © Academic Press 2020
- 19th May 2020
- Academic Press
- Paperback ISBN:
- eBook ISBN:
Dr. Ozaki received his B.Sc. in Chemistry in 1973 and PhD in Physical and Inorganic Chemistry in 1978, both from Osaka University. After over two years at the National Research Council of Canada as a research associate, he joined Jikei University School of Medicine in Tokyo. In 1989, he became associate professor and later full professor in the Chemistry Department at Kwansei Gakuin University until March 2018. He was a Vice President of Kwansei Gakuin University from April 2013 to March 2018. Currently Professor Ozaki is a professor emeritus of Kwansei Gakuin University. Ozaki has received many awards, including the 1998 Tomas Hirschfeld Award, 2001 EAS Award for Achievements in Near Infrared Spectroscopy, 2002 Spectroscopical Society of Japan Award, 2005 Science and Technology Award of Japanese Government, 2008 Japan Society for Analytical Chemistry Award, 2014 Bomem-Michelson Award, 2017 Chemical Society of Japan Award, and the 2018 Medal with Purple Ribbon.
Professor Emeritus, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo, Japan
Dr. Malgorzata Baranska leads the Raman Imaging Group at The Jagiellonian University, Kraków, Poland. In her research, Dr. Baranska focuses on analyzing bioactive compounds using spectroscopic methods, particularly modern Raman techniques. The main direction of her research is related to development treatments for life style diseases, including new drugs of endothelium bioactivity.
Professor, Faculty of Chemistry & Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Krakow, Poland
Dr. Igor Lednev received his PhD degree from The Moscow Institute of Physics and Technology, Russian Federation before joining the University of Albany as faculty. As an academic visitor, he worked in several leading laboratories around the world including the United Kingdom, Japan, Canada, and Germany. Dr. Lednev’s current research is focused on the development and application of novel laser spectroscopy for biomedical and forensic applications.
Professor, Department of Chemistry, University at Albany, State University of New York, Albany, NY, USA
Dr. Bayden R. Wood is an Australian Research Council Future Fellow working at the Centre for Biospectroscopy located in the School of Chemistry, Monash University, Malborne, Australia. His research is concerned primarily with vibrational spectroscopy of tissues, cells, and biomolecules, and monitoring home aggregation in single living red blood cells associated with erythrocyte disorders, including malaria and sickle cell disease, using resonance Raman spectroscopy.
Professor, Director, Center for Biospectroscopy, School of Chemistry, Monash University, Melbourne, VIC, Australia
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