Vibrational Spectroscopy in Protein Research

Vibrational Spectroscopy in Protein Research

From Purified Proteins to Aggregates and Assemblies

1st Edition - May 19, 2020

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  • Editors: Yukihiro Ozaki, Malgorzata Baranska, Igor Lednev, Bayden Wood
  • Paperback ISBN: 9780128186107
  • eBook ISBN: 9780128186114

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Vibrational Spectroscopy in Protein Research offers a thorough discussion of vibrational spectroscopy in protein research, providing researchers with clear, practical guidance on methods employed, areas of application, and modes of analysis. With chapter contributions from international leaders in the field, the book addresses basic principles of vibrational spectroscopy in protein research, instrumentation and technologies available, sampling methods, quantitative analysis, origin of group frequencies, and qualitative interpretation. In addition to discussing vibrational spectroscopy for the analysis of purified proteins, chapter authors also examine its use in studying complex protein systems, including protein aggregates, fibrous proteins, membrane proteins and protein assemblies. Emphasis throughout the book is placed on applications in human tissue, cell development, and disease analysis, with chapters dedicated to studies of molecular changes that occur during disease progression, as well as identifying changes in tissues and cells in disease studies.

Key Features

  • Provides thorough guidance in implementing cutting-edge vibrational spectroscopic methods from international leaders in the field
  • Emphasizes in vivo, in situ and non-invasive analysis of proteins in biomedical and life science research more broadly
  • Contains chapters that address vibrational spectroscopy for the study of simple purified proteins and protein aggregates, fibrous proteins, membrane proteins and protein assemblies


Active researchers and students in biochemistry, molecular biology, pharmaceutical science, cellular biology, applied microbiology, and biotechnology; medical chemists; clinician scientists

Table of Contents

  • 1. ATR-FTIR Spectroscopy and Spectroscopic Imaging of Proteins
    Bernadette Byrne, James W. Beattie, Cai Li Song and Sergei G. Kazarian
    2. Light-Induced Difference FTIR Spectroscopy of Photoreceptive Proteins
    Hideki Kandori and Kota Katayama
    3. Quantum Cascade Laser-based IR Transmission Spectroscopy of Proteins in Solution
    Andreas Schwaighofer and Bernhard Lendl
    4. Theoretical Simulation of Protein Two-Dimentional Infrared Spectroscopy
    Zhijun Pan, Xian Chen, Tan Jin, Lu Zhang and Wei Zhuang
    5. Infrared Spectroscopy and Imaging for Understanding Neurodegenerative Protein-Folding Diseases
    Lisa M. Miller
    6. Near-infrared (NIR) spectroscopy in protein research
    Mika Ishigaki and Yukihiro Ozaki
    7. Vibrational imaging of proteins: changes in the tissues and cells in the lifestyle disease studies
    Katarzyna Majzner, Krzysztof Czamara, Marta Z. Pacia, Jakub Dybas, Ewelina Bik, Karolina Chrabaszcz, Ewelina Wiercigroch, Aleksandra Dorosz, Adrianna Wislocka, Katarzyna M. Marzec, Kamilla Malek and Malgorzata Baranska
    8. Interpretation of Vibrational Optical Activity Spectra of Proteins
    Jana Hudecová and Petr Bouř
    9. Nano-scale analysis of protein self-assemblies
    Ewelina Lipiec
    10. Vibrational Spectroscopic Analysis and Quantification of Proteins in Human Blood Plasma and Serum
    Clement Bruno, James M. Cameron, Drishya Rajan Parachalil, Matthew J. Baker, Franck Bonnier, Holly J. Butler and Hugh J. Byrne
    11. Vibrational spectroscopy in protein research towards virus identification: challenges, new research and future perspectives
    Marfran C. D. Santos, Camilo L. M. Morais, Kássio M. G. Lima and Francis L. Martin
    12. Two-Dimensional Correlation Spectroscopy of Proteins
    Yeonju Park, Isao Noda, Young Mee Jung
    13. Resonance Raman spectroscopy of hemoglobin in red blood cells
    Bayden R. Wood, Kamila Kochan and Katarzyna M. Marzec
    14. Ultraviolet Raman spectroscopy for understanding structure and formation mechanism of amyloid fibrils
    Joseph Handen and Igor K. Lednev
    15. Recent advances in Raman spectroscopy of proteins for disease diagnosis
    Keita Iwasaki, Hemanth Noothalapati, and Tatsuyuki Yamamoto
    16. Dynamics and allostery of human hemoglobin as elucidated by time-resolved resonance Raman spectroscopy
    Yasuhisa Mizutani
    17. Immuno-SERS: from nanotag design to assays and microscopy
    Yuling Wang, Yuying Zhang and Sebastian Schlücker
    18. Highly localized characterization of protein structure and interaction by surface-enhanced Raman scattering
    Janina Kneipp
    19. Surface-enhanced Raman scattering (SERS) chemosensing of proteins
    Luca Guerrini and Ramon A. Alvarez-Puebla

Product details

  • No. of pages: 608
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: May 19, 2020
  • Imprint: Academic Press
  • Paperback ISBN: 9780128186107
  • eBook ISBN: 9780128186114

About the Editors

Yukihiro Ozaki

Dr. Ozaki received his B.Sc. in Chemistry in 1973 and PhD in Physical and Inorganic Chemistry in 1978, both from Osaka University. After over two years at the National Research Council of Canada as a research associate, he joined Jikei University School of Medicine in Tokyo. In 1989, he became associate professor and later full professor in the Chemistry Department at Kwansei Gakuin University until March 2018. He was a Vice President of Kwansei Gakuin University from April 2013 to March 2018. Currently Professor Ozaki is a professor emeritus of Kwansei Gakuin University. Ozaki has received many awards, including the 1998 Tomas Hirschfeld Award, 2001 EAS Award for Achievements in Near Infrared Spectroscopy, 2002 Spectroscopical Society of Japan Award, 2005 Science and Technology Award of Japanese Government, 2008 Japan Society for Analytical Chemistry Award, 2014 Bomem-Michelson Award, 2017 Chemical Society of Japan Award, and the 2018 Medal with Purple Ribbon.

Affiliations and Expertise

Professor Emeritus, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo, Japan

Malgorzata Baranska

Dr. Malgorzata Baranska leads the Raman Imaging Group at The Jagiellonian University, Kraków, Poland. In her research, Dr. Baranska focuses on analyzing bioactive compounds using spectroscopic methods, particularly modern Raman techniques. The main direction of her research is related to development treatments for life style diseases, including new drugs of endothelium bioactivity.

Affiliations and Expertise

Professor, Faculty of Chemistry & Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Krakow, Poland

Igor Lednev

Dr. Igor Lednev received his PhD degree from The Moscow Institute of Physics and Technology, Russian Federation before joining the University of Albany as faculty. As an academic visitor, he worked in several leading laboratories around the world including the United Kingdom, Japan, Canada, and Germany. Dr. Lednev’s current research is focused on the development and application of novel laser spectroscopy for biomedical and forensic applications.

Affiliations and Expertise

Professor, Department of Chemistry, University at Albany, State University of New York, Albany, NY, USA

Bayden Wood

Dr. Bayden R. Wood is an Australian Research Council Future Fellow working at the Centre for Biospectroscopy located in the School of Chemistry, Monash University, Malborne, Australia. His research is concerned primarily with vibrational spectroscopy of tissues, cells, and biomolecules, and monitoring home aggregation in single living red blood cells associated with erythrocyte disorders, including malaria and sickle cell disease, using resonance Raman spectroscopy.

Affiliations and Expertise

Professor, Director, Center for Biospectroscopy, School of Chemistry, Monash University, Melbourne, VIC, Australia

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