Vaccinology and Methods in Vaccine Research

Vaccinology and Methods in Vaccine Research

1st Edition - March 10, 2022

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  • Editors: Rebecca Ashfield, Angus Oli, Charles Esimone, Linda Anagu
  • eBook ISBN: 9780323914383
  • Paperback ISBN: 9780323911467

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Description

Vaccinology and Methods in Vaccine Research is a combination of cutting-edge methodologies, experimental approaches and literature reviews. The book covers all aspects of vaccine development, including basic immunology (focusing on the stimulation of adaptive immunity, which is required for vaccine efficacy), approaches to vaccine design and target validation, vaccine biomanufacturer and clinical development. Existing vaccinology resources are theoretical reference books, whereas this book provides a practical handbook for use in the research lab and classroom by those working in vaccinology and training others in the field. It is authored and edited by scientists actively engaged in vaccine research and development for day-to-day teaching/methodological advice.

Key Features

  • Addresses how to design a vaccine for an emerging disease, from a practical point-of -view, with chapters written by scientists who are grappling with these questions
  • Provides new approaches to vaccine development. including vaccine targeting and virus-like-particle vaccines
  • Gives up-to-date information and methodologies in use for vaccine adjuvants

Readership

Academic faculties, researchers, students and scientists in the field of immunology, pharmacology and vaccinology; Academics researching and developing vaccines (from graduate students to group leaders)

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • List of contributors
  • Preface
  • Acknowledgements
  • Chapter 1. Immunology and the concept of vaccination
  • Abstract
  • Manipulation of the immune system by vaccination
  • Overview of immunity and the immune system
  • Prophylactic and therapeutic vaccines
  • References
  • Chapter 2. Vaccine types and reverse vaccinology
  • Abstract
  • Introduction
  • Fundamentals of vaccine immunology
  • Classes of vaccines
  • Immunoinformatics
  • References
  • Chapter 3. Dendritic cells as vaccine targets
  • Abstract
  • Introduction to dendritic cells
  • Origin and types of dendritic cells
  • Location of dendritic cells
  • Receptors of dendritic cells
  • References
  • Further reading
  • Chapter 4. Vaccine development: from the laboratory to the field
  • Abstract
  • Introduction
  • Antigen selection for new vaccines
  • Genetic studies
  • Basic protein studies
  • Other methods of in vitro evaluation of immunogenicity
  • Conclusion
  • References
  • Further reading
  • Chapter 5. Harnessing the power of CD8+ T-cells: identification and validation of peptides bound to major histocompatibility complex class I by immunopeptidomics
  • Abstract
  • CD8+ T cells and major histocompatibility complex class I antigen presentation
  • Proteasome cleavage mechanisms
  • Intracellular trafficking of peptides
  • Peptide binding affinity to major histocompatibility complex class I
  • Tools for discovering immunogenic peptides
  • Immunopeptidomics
  • Vaccine design based on immunopeptidomics
  • Methods for the validation of immunogenic peptides
  • Conclusions
  • Acknowledgments
  • References
  • Chapter 6. Virus-like particle vaccines
  • Abstract
  • Introduction
  • Immune response to virus-like particles
  • Production of virus-like particles
  • Expression systems
  • Manufacturing: upstream process
  • Manufacturing: purification of virus-like particles (downstream process)
  • Major contaminants and removal strategies
  • Analytical tools for virus-like particles
  • Conclusion
  • References
  • Chapter 7. Approaches to cancer vaccination
  • Abstract
  • Introduction
  • Types of cancer vaccines
  • Novel trends in cancer vaccine development
  • Cancer vaccine delivery systems
  • Summary
  • References
  • Chapter 8. Vaccines against SARS-CoV-2
  • Abstract
  • The COVID-19 pandemic
  • The spike protein of the SARS-CoV-2 virus
  • COVID-19 vaccine development
  • Nucleic acid–based vaccines
  • Viral vector vaccines
  • Antigen-presenting cell-based vaccines
  • Whole organism-based vaccines
  • Future vaccine requirements and challenges
  • References
  • Chapter 9. Adjuvants, immunomodulators, and adaptogens
  • Abstract
  • Introduction
  • Aluminum-based adjuvants
  • Emulsion-based adjuvants
  • Saponins
  • Immune stimulating complexes
  • TLR agonists
  • Nanoparticles
  • Liposomes
  • Exosomes
  • GSK adjuvant systems
  • Cholera toxin
  • Freund’s adjuvant
  • α-GalCer
  • Adjuvant properties of the microbiome
  • Plant-derived adaptogens
  • References
  • Chapter 10. GMP manufacture and quality control
  • Abstract
  • Introduction
  • Regulation
  • GMP manufacture
  • Vaccine purification
  • In process controls and final release testing
  • References
  • Chapter 11. Testing vaccines: essential steps in clinical trial design
  • Abstract
  • Introduction
  • Vaccine development pathway
  • Introduction to good clinical practice
  • Consent
  • Good clinical practice compliant documentation
  • Clinical trial roles and responsibilities
  • Ensuring quality
  • Safety
  • Adverse events
  • Other trial considerations
  • Final word
  • References
  • Index

Product details

  • No. of pages: 336
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: March 10, 2022
  • Imprint: Academic Press
  • eBook ISBN: 9780323914383
  • Paperback ISBN: 9780323911467

About the Editors

Rebecca Ashfield

Rebecca Ashfield Jenner Institute, University of Oxford (ORCRB Roosevelt Drive Oxford OX3 7DQ). BA (1st class) Oxford, DPhil Oxford, over 20 years working in field of immunotherapy and vaccine development. Currently CSO of Ducentis Biotherapeutics Ltd and Senior Project Manager at the Jenner Institute. Organiser/co-leader of several vaccinology courses in Oxford and Leiden. Edited most recent Jenner Institute triennial report. Expertise in vaccine GMP manufacture, led successful application to Innovate UK for £75M vaccine innovation and manufacturing centre, currently being built at Harwell in Oxfordshire. H=20

Affiliations and Expertise

Currently CSO of Ducentis Biotherapeutics Ltd and Senior Project Manager, Jenner Institute, University of Oxford, UK

Angus Oli

Angus Nnamdi Oli Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University (NAU), Agulu Campus (Post code 422001, Agulu, Anambra State, Nigeria). BPharm. and MPharm. UNN, DPhil NAU and a DPharm. University of Benin, Nigeria. Currently a Senior Lecturer with over 8 years’ experience. Author of several articles in peer-reviewed journals indexed in Pubmed, Web of Science and Scopus. Current research interests/career goals are Infection and immunology of infectious diseases with emphasis of viral and bacterial infections, development of effective vaccines with strong antibody and T lymphocytes effect or responses against the infections, Pharmaceutical Quality Systems and Clinical vaccinology. H: 5

Affiliations and Expertise

Senior Lecturer, Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University (NAU), Agulu Campus, Agulu, Anambra State, Nigeria

Charles Esimone

Charles Okechukwu Esimone Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University (NAU, Main Campus, PMB 5025, Awka, Anambra State, Nigeria). BPharm., MPharm. and PhD in Pharmaceutics, University of Nigeria, Nsukka (UNN). With over 20 years’ experience as a lecturer and research scientist and over 12 years’ experience in vaccinology/immunology research. Currently a Professor and VC of Nnamdi Azikiwe University, Editor-in-Chief of the Journal of Current Biomedical Research and the Biomedical Research Committee, currently chaired by me, focuses on the development of biotechnological products such as vaccines for tropical diseases, amongst other interest. H: 18

Affiliations and Expertise

Lecturer and Senior Scientist, Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University (NAU, Main Campus, PMB 5025, Awka, Anambra State, Nigeria). BPharm., MPharm. and PhD in Pharmaceutics, University of Nigeria, Nsukka (UNN), Nigeria

Linda Anagu

Linda Onyeka Anagu Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University (NAU), Agulu Campus (Post code 422001, Agulu, Anambra State, Nigeria). BPharm. and MPharm. UNN, DPhil Keele University. With over 8 years’ experience as a lecturer and research scientist. She has interest in vaccine design/development processes that borders on her expertise in molecular cloning and Plasmodium epigenetics. Recently, attended the Vaccinology Summer School organized by Dr. Rebecca Ashfield and the late Dr. Sahid Khan in Leiden. Edited the recent, 2015 edition of LABORATORY HANDBOOK OF PHARMACEUTICAL MICROBIOLOGY AND BIOTECHNOLOGY, used by undergraduate and postgraduate students at Nnamdi Azikiwe University. ERC H: 5

Affiliations and Expertise

Lecturer and Research Scientist, Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University (NAU), Agulu Campus, Agulu, Anambra State, Nigeria). BPharm. and MPharm. UNN, DPhil Keele University, Nigeria

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