Ultracentrifugation of Macromolecules

Ultracentrifugation of Macromolecules: Modern Topics covers the theoretical description of the problem of sedimentation equilibrium of polydisperse nonideal solutions. The book discusses the sedimentation equilibrium in polydisperse nonideal solutions and the size distribution analysis by ultracentrifugal methods. The text also describes the simultaneous sedimentation and equilibrium in beta-lactoglobulin B, chymotrypsinogen A, and lysozyme solutions; as well as the sedimentation analysis of a multiple myeloma γG-globulin. People involved in physical biochemistry will find the book invaluable.

Foreword Preface Acknowledgements List of Symbols Part 1 Polydisperse Solute Systems Chapter I. Sedimentation Equilibrium in Polydisperse Nonideal Solutions History Theory Sedimentation Equilibrium Experiments Summary References Chapter II. Size Distribution Analysis by Ultracentrifugal Methods Theory Experiment I. Polystyrene-Cyclohexane at 34.2°C Experiment II. Dextran-Water at 25°C Summary References Part 2. Self-Association Reactions in Protein Systems Chapter III. Simultaneous Sedimentation and Chemical Equilibrium in ß-Lactoglobulin B, Chymotrypsinogen A, and Lysozyme Solutions ß-Lactoglobulin B Lysozyme Chymotrypsinogen A Summary References Chapter IV. Sedimentation Analysis of a Multiple Myeloma γG-Globulin Theory Experimental Results Correlation of the Data Summary and Concluding Remarks References Part 3 Appendixes Appendix A. A Brief Introduction to the Theory of Ultracentrifugai Analysis Basic Principles Sedimentation Equilibrium Sedimentation Transport References Bibliography Appendix B. Molecular Homogeneity and Its Demonstration Sedimentation Velocity Sedimentation Equilibrium References Author Index Subject Index