Ultracentrifugation of Macromolecules

Ultracentrifugation of Macromolecules: Modern Topics covers the theoretical description of the problem of sedimentation equilibrium of polydisperse nonideal solutions. The book discusses the sedimentation equilibrium in polydisperse nonideal solutions and the size distribution analysis by ultracentrifugal methods. The text also describes the simultaneous sedimentation and equilibrium in beta-lactoglobulin B, chymotrypsinogen A, and lysozyme solutions; as well as the sedimentation analysis of a multiple myeloma γG-globulin. People involved in physical biochemistry will find the book invaluable.

Foreword

Preface

Acknowledgements

List of Symbols

Part 1 Polydisperse Solute Systems

Chapter I. Sedimentation Equilibrium in Polydisperse

Nonideal Solutions

History

Theory

Sedimentation Equilibrium Experiments

Summary

References

Chapter II. Size Distribution Analysis by Ultracentrifugal Methods

Theory

Experiment I. Polystyrene-Cyclohexane at 34.2°C

Experiment II. Dextran-Water at 25°C

Summary

References

Part 2. Self-Association Reactions in Protein Systems

Chapter III. Simultaneous Sedimentation and Chemical Equilibrium in ß-Lactoglobulin B, Chymotrypsinogen A, and Lysozyme Solutions

ß-Lactoglobulin B

Lysozyme

Chymotrypsinogen A

Summary

References

Chapter IV. Sedimentation Analysis of a Multiple Myeloma γG-Globulin

Theory

Experimental Results

Correlation of the Data

Summary and Concluding Remarks

References

Part 3 Appendixes

Appendix A. A Brief Introduction to the Theory of Ultracentrifugai Analysis

Basic Principles

Sedimentation Equilibrium

Sedimentation Transport

References

Bibliography

Appendix B. Molecular Homogeneity and Its Demonstration

Sedimentation Velocity

Sedimentation Equilibrium

References

Author Index

Subject Index