Ultracentrifugation of Macromolecules - 1st Edition - ISBN: 9780127551500, 9781483220628

Ultracentrifugation of Macromolecules

1st Edition

Modern Topics

Authors: J. W. Williams
eBook ISBN: 9781483220628
Imprint: Academic Press
Published Date: 1st January 1972
Page Count: 136
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Description

Ultracentrifugation of Macromolecules: Modern Topics covers the theoretical description of the problem of sedimentation equilibrium of polydisperse nonideal solutions. The book discusses the sedimentation equilibrium in polydisperse nonideal solutions and the size distribution analysis by ultracentrifugal methods. The text also describes the simultaneous sedimentation and equilibrium in beta-lactoglobulin B, chymotrypsinogen A, and lysozyme solutions; as well as the sedimentation analysis of a multiple myeloma γG-globulin. People involved in physical biochemistry will find the book invaluable.

Table of Contents

Foreword Preface Acknowledgements List of Symbols Part 1 Polydisperse Solute Systems Chapter I. Sedimentation Equilibrium in Polydisperse Nonideal Solutions History Theory Sedimentation Equilibrium Experiments Summary References Chapter II. Size Distribution Analysis by Ultracentrifugal Methods Theory Experiment I. Polystyrene-Cyclohexane at 34.2°C Experiment II. Dextran-Water at 25°C Summary References Part 2. Self-Association Reactions in Protein Systems Chapter III. Simultaneous Sedimentation and Chemical Equilibrium in ß-Lactoglobulin B, Chymotrypsinogen A, and Lysozyme Solutions ß-Lactoglobulin B Lysozyme Chymotrypsinogen A Summary References Chapter IV. Sedimentation Analysis of a Multiple Myeloma γG-Globulin Theory Experimental Results Correlation of the Data Summary and Concluding Remarks References Part 3 Appendixes Appendix A. A Brief Introduction to the Theory of Ultracentrifugai Analysis Basic Principles Sedimentation Equilibrium Sedimentation Transport References Bibliography Appendix B. Molecular Homogeneity and Its Demonstration Sedimentation Velocity Sedimentation Equilibrium References Author Index Subject Index

Details

No. of pages:
136
Language:
English
Copyright:
© Academic Press 1972
Published:
Imprint:
Academic Press
eBook ISBN:
9781483220628

About the Author

J. W. Williams

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