Ubiquitin-dependent Protein Degradation

Ubiquitin-dependent Protein Degradation

1st Edition - March 23, 2019
This is the Latest Edition
  • Editor: Mark Hochstrasser
  • Hardcover ISBN: 9780128186671
  • eBook ISBN: 9780128186688

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Description

Ubiquitination and Protein Stability - Part B, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Topics of note include chapters on Assays of SUMO protease function in mammalian cells, In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation, Methods to study proteasome regulatory particle assembly, Native mass spectrometry approaches to study the proteasome, Single-molecule methods to study the ubiquitin-proteasome system, Assays for the function of ubiquitin in the mammalian endocytic pathway, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology

Readership

Biochemists, chemists, microbiologists, microbiome specialists, marine scientists, protein chemists, biotechnology, chemical biologists, blue biotech experts, natural product chemists, drug discovery professionals.

Table of Contents

  • 1. Assays for protein retrotranslocation in ERAD
    Sonya Neal, Sascha H. Duttke and Randolph Y. Hampton
    2. Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae
    Rahul S. Samant and Judith Frydman
    3. Methodologies to monitor protein turnover at the inner nuclear membrane
    Pei-Ling Tsai, Chenguang Zhao and Christian Schlieker
    4. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7
    Bayan Mashahreh, Yuval Reiss, Reuven Wiener and Tommer Ravid
    5. Methods for genetic analysis of mammalian ER-associated degradation
    Dara E. Leto and Ron R. Kopito
    6. A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies
    Néstor García-Rodríguez and Helle D. Ulrich
    7. Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome
    Randi G. Reed and Robert J. Tomko Jr.
    8. Assays for ubiquitin-like protein ligation and proteasome function in archaea
    Xian Fu, Zachary Adams and Julie Maupin-Furlow
    9. Structural mass spectrometry approaches to study the 20S proteasome
    Gili Ben-Nissan, Shay Vimer, Mark Tarnavsky and Michal Sharon
    10. Single-molecule methods for measuring ubiquitination and protein stability
    Jason Hon and Ying Lu
    11. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation
    Srinivasan Muniyappan and Byung-Hoon Lee
    12. Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway
    Evan L. Guiney, Lu Zhu, Richa Sardana, Scott D. Emr and Matthew G. Baile
    13. Detection of ubiquitinated targets in mammalian and Drosophila models
    Elena Maspero, Valentina Fajner, Janine Weber and Simona Polo
    14. Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans
    Alexander Springhorn and Thorsten Hoppe
    15. Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells
    Victoria Cohen-Kaplan, Ido Livneh, Yong Tae Kwon and Aaron Ciechanover

Product details

  • No. of pages: 384
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: March 23, 2019
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128186671
  • eBook ISBN: 9780128186688
  • About the Serial Volume Editor

    Mark Hochstrasser

    Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology Yale University, USA.

    Affiliations and Expertise

    Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology, Yale University, USA