Ubiquitin and Ubiquitin-like Protein Modifiers

Ubiquitin and Ubiquitin-like Protein Modifiers

1st Edition - March 6, 2019

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  • Editor: Mark Hochstrasser
  • eBook ISBN: 9780128163603
  • Hardcover ISBN: 9780128163597

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Ubiquitination and Protein Stability - Part A Volume 618, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more. 

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics


Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech specialists, natural product chemists, drug discovery experts

Table of Contents

  • 1. Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage
    Michael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger
    2. Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extension
    Daniel C. Scott and Brenda A. Schulman
    3. A genetic approach to study polyubiquitination in Saccharomyces cerevisiae
    Fernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski
    4. Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteins
    Viduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden
    5. Methods to measure ubiquitin chain length and linkage
    Fumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki
    6. Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBE
    Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka
    7. Methods to study phosphoribosylated ubiquitin ligation and removal
    Jiazhang Qiu and Zhao-Qing Luo
    8. Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assays
    Nathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler
    9. Methods to study SUMO dynamics in yeast
    Stefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen
    10. Approaches for investigating the extracellular signaling function of ISG15
    Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse
    11. Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10
    Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup
    12. Methods to analyze STUbL activity
    Emma Branigan, Anna Plechanovová and Ronald T. Hay
    13. Quantitative analysis of USP activity in vitro
    Shreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma
    14. Evaluating enzyme activities and structures of DUBs
    Jonathan N. Pruneda and David Komander
    15. Purification and functional characterization of the DUB domain of SdeA
    Kedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das
    16. Profiling DUBs and Ubl-specific proteases with activity-based probes
    Paul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa
    17. Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues
    Kathrin Kunz, Stefan Müller and Luca Mendler

Product details

  • No. of pages: 430
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: March 6, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128163603
  • Hardcover ISBN: 9780128163597

About the Serial Volume Editor

Mark Hochstrasser

Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology Yale University, USA.

Affiliations and Expertise

Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology, Yale University, USA

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