Ubiquitin and Ubiquitin-like Protein Modifiers - 1st Edition - ISBN: 9780128163597

Ubiquitin and Ubiquitin-like Protein Modifiers, Volume 618

1st Edition

Serial Volume Editors: Mark Hochstrasser
Hardcover ISBN: 9780128163597
Imprint: Academic Press
Published Date: 15th March 2019
Page Count: 412
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Table of Contents

1. Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages
Cynthia Wolberger
2. Methods to measure ubiquitin chain length and linkage
Yasushii Saeki
3. Genetic approaches to study the yeast ubiquitin system
David Toczyski
4. Enzymatic preparation of monoubiquitinated proteins
Helen Walden
5. Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway
Aaron Ciechanover
6. Purification and characterization of enzyme activity of USPs
Titia k. Sixma
7. Evaluation of enzyme activities and structures of DUBs
David Komander
8. Structural analysis of JAMM/MPN+ DUBs
Chittaranjan Das
9. Biochemical methods to study SUMO conjugation
Andrea Pichler
10. Methods to study SUMO dynamics in yeast
R. Jürgen Dohmen
11. Approaches to study signaling functions of ISG15
Jon Huibregste
12. Methods to analyze STUbL activity
Ron Thomas Hay
13. Assays of SUMO protease function in mammalian cells
Stefan Müller


Description

Ubiquitination and Protein Stability - Part A Volume 618, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more. 

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on methods to measure ubiquitin chain length and linkage, genetic approaches to study the yeast ubiquitin system, amongst many other timely topics

Readership

Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech specialists, natural product chemists, drug discovery experts


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128163597

Reviews

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Ratings and Reviews


About the Serial Volume Editors

Mark Hochstrasser Serial Volume Editor

Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology Yale University, USA.

Affiliations and Expertise

Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental Biology, Yale University, USA