Description

Ubiquitin and Protein Degradation, Part B will cover chemical biology, ubiquitin derivatives and ubiquitin-like proteins, deubiquitinating enzymes, proteomics as well as techniques to monitor protein degradation. The chapters are highly methodological and focus on application of techniques.

Key Features

*Second part of the Ubiquitin and Protein Degration series *Topics include: E1 Enzymes, E2 Enzymes, E3 Enzymes, Proteasomes, and Isopeptidases

Readership

Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Table of Contents

SECTION I. UBIQUITIN AND UBIQUITIN DERIVATIVES Chapter 1. Chemical and genetic strategies for manipulating polyubiquitin chain structure; Chapter 2. Controlled synthesis of polyubiquitin chains; Chapter 3. Derivitization of the C-terminus of ubiquitin and ubiquitin-like proteins using Intein chemistry: Methods & Uses; Chapter 4. Preparation, characterization, and use of tagged ubiquitin; Chapter 5. Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically-encodable tandem ubiquitin; Chapter 6. Production of anti-polyubiquitin antibodies and their use for characterization and isolation of polyubiquitinated proteins; Chapter 7. Application of Ubiquitin Immunohistochemistry to Diagnosis of Disease; Chapter 8. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling and protease identification SECTION II. UBIQUITIN-BINDING DOMAINS Chapter 9. Identification and characterization of modular domains that bind ubiquitin; Chapter 10. Analysis of Ubiquitin chain-binding proteins by two-hybrid methods; Chapter 11. Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance; Chapter 12. Using NMR spectroscopy to monitor ubiquitin chain conformation and interactions with ubiquitin-binding domains; Chapter 13. Analysis of ubquitin-dependent protein sorting within the endocytic pathway in Saccharomyces cerevisiae SECTION III. METHODS TO STUDY THE PROTEASOME Chapter 14. Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity; Chapter 15. Large- and Small-scale Purification of Mammalian 26S Proteasomes SECTION IV. IDENTIFICATION AND CHARACTERIZATION OF SUBSTRATES AND UBIQUITIN LIGA

Details

No. of pages:
952
Language:
English
Copyright:
© 2005
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080884288
Print ISBN:
9780121828042

Reviews

Praise for the Series: @quote:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @quote:"The Methods in Enzymology series represents the gold standard." @source:--NEUROSCIENCE