Two-Component Signaling Systems, Part B, Volume 423

1st Edition

Serial Volume Editors: Melvin Simon Brian Crane Alexandrine Crane
Hardcover ISBN: 9780123738523
eBook ISBN: 9780080549460
Imprint: Academic Press
Published Date: 3rd July 2007
Page Count: 648
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Table of Contents

  • Contributors to Volume 423
    • Publisher Summary
  • Contents of Previous Volumes
  • Section I: Structural Approaches
    • [1]: The PICM Chemical Scanning Method for Identifying Domain–Domain and Protein–Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis
      • Abstract
      • Introduction
      • Comparison of the PICM Method with Other Scanning Approaches
      • PICM Studies of the Core Signaling Complex of Bacterial Chemotaxis
      • Generalizing the PICM Method to Map Docking Sites in Other Systems
      • Incorporation of an Affinity Tag and Creation of a Cysless Protein
      • Choice of Positions for Cys Incorporation and Creation of a Mutant Library
      • Selection of a Cys-Specific Probe for Chemical Modification
      • Probe Labeling and Purification of the Single Cys Mutants
      • Quantitation of Probe Coupling
      • Measuring Functional Effects of Cys Substitution and Bulky Probe Coupling
      • Interpretation of Results—Mapping Out Docking Sites
      • Acknowledgments
    • [2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis
      • Abstract
      • Introduction
      • Site-Directed Cysteine and Disulfide Chemistry: History
      • Site-Directed Cysteine and Disulfide Chemistry: Applications and Limitations
      • Incorporation of an Affinity Tag and Creation of a Cysless Protein
      • Choice of Positions for Cys Incorporation and Creation of a Mutant Library
      • Analysis of 2° Structure by Chemical Reactivity Scanning
      • Disulfide Mapping of Spatial Proximity and Conformational Changes
      • Disulfide Trapping of Thermal Backbone and Domain Motions
      • Acknowledgments
    • [3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine

Description

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections include: Structural Approaches Reconstitution of Heterogeneous Systems Intracellular Methods and Assays Genome-Wide Analyses of Two-Component Systems

Key Features

Presents detailed protocols Includes troubleshooting tips

Readership

Biochemists, geneticists, and molecular biologists.


Details

No. of pages:
648
Language:
English
Copyright:
© Academic Press 2007
Published:
Imprint:
Academic Press
eBook ISBN:
9780080549460
Hardcover ISBN:
9780123738523

About the Serial Volume Editors

Melvin Simon Serial Volume Editor

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

Brian Crane Serial Volume Editor

Affiliations and Expertise

Cornell University, Ithaca, NY, USA

Alexandrine Crane Serial Volume Editor

Affiliations and Expertise

Cornell University, Ithaca, NY, USA