Tumor Immunology and Immunotherapy – Molecular Methods

Tumor Immunology and Immunotherapy – Molecular Methods

1st Edition - November 13, 2019
This is the Latest Edition
  • Editors: Lorenzo Galluzzi, Nils-Petter Rudqvist
  • Hardcover ISBN: 9780128186718
  • eBook ISBN: 9780128186725

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Tumor Immunology and Immunotherapy – Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more.

Key Features

  • Contains the authority of authors who are leaders in their field
  • Provides a comprehensive source on new methods and research in enzymology


This volume is addressed to experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques

Table of Contents

  • 1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies

    Sara Valpione and Luca Campana

    2. Detection and quantification of cytosolic DNA

    Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box

    3. Methods to detect endogenous dsRNA induction and recognition

    Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho

    4. Quantification of eIF2alpha phosphorylation during immunogenic cell death

    Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp

    5. Assessment of annexin A1 release during immunogenic cell death

    Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer

    6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells

    George R. Dubyak

    7. Quinacrine-mediated detection of intracellular ATP

    Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp

    8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells

    Francesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi

    9. LEGENDplex™: Bead-assisted multiplex cytokine profiling by flow cytometry

    Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun

    10. Methods for measuring HMGB1 release during immunogenic cell death

    Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer

    11. Different measures of HMGB1 location in cancer immunology

    Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze

    12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues

    Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast

    13. Quantification of IDO1 enzyme activity in normal and malignant tissues

    Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright

    14. Enzyme activity of circulating CD73 in human serum

    Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto and
    Mariaelena Capone

    15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays

    Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg

    16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis

    Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik

    17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization

    Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris

    18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1

    Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao

    19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies

    Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata

    20. High-throughput discovery of cancer-targeting TCRs

    Lukas Bunse, Edward W. Green and Michael Platten

    21. TCR validation toward gene therapy for cancer

    Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten

    22. Analysis of TCR β CDR3 sequencing data for tracking anti-tumor immunity

    Jiajia Zhang, Zhicheng Ji and Kellie N. Smith

    23. Quantitative analysis of the T cell receptor repertoire

    Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain

    24. CRATES: A one-step assembly method for Class 2 CRISPR arrays

    Chunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel

Product details

  • No. of pages: 552
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: November 13, 2019
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128186718
  • eBook ISBN: 9780128186725
  • About the Serial Volume Editors

    Lorenzo Galluzzi

    Lorenzo Galluzzi
    Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

    Affiliations and Expertise

    Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

    Nils-Petter Rudqvist

    Nils-Petter Rudqvist
    Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

    Affiliations and Expertise

    Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA