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Tumor Immunology and Immunotherapy – Molecular Methods - 1st Edition - ISBN: 9780128186718, 9780128186725

Tumor Immunology and Immunotherapy – Molecular Methods, Volume 629

1st Edition

Serial Volume Editors: Lorenzo Galluzzi Nils-Petter Rudqvist
Hardcover ISBN: 9780128186718
eBook ISBN: 9780128186725
Imprint: Academic Press
Published Date: 13th November 2019
Page Count: 552
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Table of Contents

1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies

Sara Valpione and Luca Campana

2. Detection and quantification of cytosolic DNA

Sheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box

3. Methods to detect endogenous dsRNA induction and recognition

Ilias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho

4. Quantification of eIF2alpha phosphorylation during immunogenic cell death

Lucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp

5. Assessment of annexin A1 release during immunogenic cell death

Elisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer

6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cells

George R. Dubyak

7. Quinacrine-mediated detection of intracellular ATP

Sabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp

8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cells

Francesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi

9. LEGENDplex™: Bead-assisted multiplex cytokine profiling by flow cytometry

Jason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun

10. Methods for measuring HMGB1 release during immunogenic cell death

Liwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer

11. Different measures of HMGB1 location in cancer immunology

Carolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze

12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissues

Sunil Thomas, Lisa Laury-Kleintop and George C. Prendergast

13. Quantification of IDO1 enzyme activity in normal and malignant tissues

Lijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright

14. Enzyme activity of circulating CD73 in human serum

Silvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto and
Mariaelena Capone

15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assays

Bertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg

16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosis

Jesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik

17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization

Leepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris

18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1

Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao

19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodies

Norimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata

20. High-throughput discovery of cancer-targeting TCRs

Lukas Bunse, Edward W. Green and Michael Platten

21. TCR validation toward gene therapy for cancer

Edward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten

22. Analysis of TCR β CDR3 sequencing data for tracking anti-tumor immunity

Jiajia Zhang, Zhicheng Ji and Kellie N. Smith

23. Quantitative analysis of the T cell receptor repertoire

Imran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain

24. CRATES: A one-step assembly method for Class 2 CRISPR arrays

Chunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel


Tumor Immunology and Immunotherapy – Molecular Methods, Volume 629, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this release include Droplet digital PCR for measuring circulating tumor-derived DNA, Detection and quantification of cytosolic DNA, Methods to detect endogenous dsRNA induction and recognition, Quantification of eIF2alpha phosphorylation during immunogenic cell death, Assessment of annexin A1 release during immunogenic cell death, Luciferase-assisted detection of extracellular ATP in the course of ICD, The P2X7 receptor: structure and function, and much more.

Key Features

  • Contains the authority of authors who are leaders in their field
  • Provides a comprehensive source on new methods and research in enzymology


This volume is addressed to experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques


No. of pages:
© Academic Press 2019
13th November 2019
Academic Press
Hardcover ISBN:
eBook ISBN:

Ratings and Reviews

About the Serial Volume Editors

Lorenzo Galluzzi

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and Expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

Nils-Petter Rudqvist

Nils-Petter Rudqvist

Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

Affiliations and Expertise

Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA