Tumor Immunology and Immunotherapy - Integrated Methods Part B
 - 1st Edition - ISBN: 9780128206676

Tumor Immunology and Immunotherapy - Integrated Methods Part B , Volume 636

1st Edition

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Serial Volume Editors: Lorenzo Galluzzi Nils-Petter Rudqvist
Hardcover ISBN: 9780128206676
Imprint: Academic Press
Published Date: 1st April 2020
Page Count: 406
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Table of Contents

1. A comprehensive approach to gene expression profiling in immune cells
Sabrina Carpentier, François Romagné and Eric Vivier
2. DIY: Visualizing the immune landscape of tumors using transcriptome and methylome data
Steven F. Gameiro, Farhad Ghasemi, John W. Barrett, James Koropatnick, Anthony C. Nichols, Joe S. Mymryk and Saman Maleki Vareki
3. Using epigenetic data to estimate immune composition in admixed samples
Ankur Chakravarthy and Daniel De Carvalho
4. Metabolomic approaches to study the tumor microenvironment
Giuseppe Astarita, Suraj Dhungana, Bindesh Shrestha and Evagelia C. Laiakis
5. Immunoscore assay for the immune classification of solid tumors: Technical aspects, improvements and clinical perspectives
Florence Marliot, Lucie Lafontaine and Jérôme Galon
6. Quantification methods of transforming growth factor beta (TGF-β) activity in the setting of cancer immunotherapy
Gabriel Gallo-Oller, Marianna Di Scala, Fernando Aranda and Javier Dotor
7. Isolation of DNA from exosomes
Sheila Spada, Nils Rudqvist and Erik Wennerberg
8. Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses
Shentong Fang, Patrizia Agostinis, Petri Salven and Abhishek D. Garg
9. Computational methods in tumor immunology
Bhavneet Bhinder and Olivier Elemento
10. Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseq
Christina Plattner, Francesca Finotello and Dietmar Rieder
11. Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancers
Ann-Marie Baker and Trevor A. Graham
12. RNA interference screening methods to identify proliferation determinants and mechanisms of resistance to immune attack
Yong-Wei Zhang, Rochelle E. Nasto, Sandra A. Jablonski, Ilya G. Serebriiskii, Rishi Surana, Joseph Murray, Michael Johnson, Rebecca B. Riggins, Robert Clarke, Erica A. Golemis and
Louis M. Weiner
13. An altered miTRAP method for miRNA affinity purification with its pros and cons
Simon Jasinski-Bergner, Christoforos Vaxevanis, Nadine Heimer, Maria-Filothei Lazaridou, Michael Friedrich and Barbara Seliger
14. Identification of immunomodulatory RNA-binding proteins in tumors
Michael Friedrich, Maria-Filothei Lazaridou, Jette Rahn, Christoforos Vaxevanis, Nadine Heimer, Simon Jasinski-Bergner and Barbara Seliger
15. Techniques for the generation of humanized models for immuno-oncology
Chun I. Yu, Florentina Marches, Te-Chia Wu, Jan Martinek and Karolina Palucka


Tumor Immunology and Immunotherapy - Integrated Methods Part B, Volume 636 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this update include Quantification methods of Transforming Growth Factor beta (TGF˗β) activity in the setting of cancer immunotherapy, Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses, Tracking and interrogating tissue-resident and recruited microglia in brain tumors, Metabolomics and lipidomics of the tumor microenvironment, Monitoring abscopal responses to radiation in mice, and much more.

Key Features

  • Provides an array of authors who are authorities in the field
  • Presents comprehensiveness coverage of the topics
  • Includes a broad level of detail and in-depth coverage


This volume is addressed to experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques


No. of pages:
© Academic Press 2020
1st April 2020
Academic Press
Hardcover ISBN:

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About the Serial Volume Editors

Lorenzo Galluzzi

Lorenzo Galluzzi Serial Volume Editor

Lorenzo Galluzzi (born 1980) is currently Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College (New York, USA), and Honorary Associate Professor at the Faculty of Medicine of the Paris Descartes University (Paris, France). Prior to joining Weill Cornell Medical College (2017), Lorenzo Galluzzi was a Junior Scientist of the Research Team “Apoptosis, Cancer and Immunity” at the Cordeliers Research Center (Paris, France; 2012-2016). Lorenzo Galluzzi did his post-doctoral training at the Gustave Roussy Cancer Center (Villejuif, France; 2009-2011), after receiving his PhD from the Paris Sud University (Le Kremlin-Bicetre, France; 2005-2008). He is also Associate Director of the European Academy for Tumor Immunology (EATI), and Founding Member of the European Research Institute for Integrated Cellular Pathology (ERI-ICP). Lorenzo Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. In particular, he provided profound insights into the links between adaptive stress responses in cancer cells and the activation of a clinically relevant tumor-targeting immune response in the context of chemotherapy and radiation therapy. Lorenzo Galluzzi has published more than 350 scientific articles in international peer-reviewed journals. According to a survey published by Lab Times, he is currently the 6th and the youngest of the 30 most-cited European cell biologists (relative to the period 2007–2013). Lorenzo Galluzzi currently operates as Editor-in-Chief of three journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, and Molecular and Cellular Oncology (which he co-founded in 2013). In addition, Lorenzo Galluzzi currently serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease.

Affiliations and Expertise

Weill Cornell Medical College, USA

Nils-Petter Rudqvist

Nils-Petter Rudqvist Serial Volume Editor

Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

Affiliations and Expertise

Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA