Tumor Immunology and Immunotherapy - Integrated Methods Part A

Tumor Immunology and Immunotherapy - Integrated Methods Part A

1st Edition - February 28, 2020
This is the Latest Edition
  • Editors: Lorenzo Galluzzi, Nils-Petter Rudqvist
  • eBook ISBN: 9780128188750
  • Hardcover ISBN: 9780128186770

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Tumor Immunology and Immunotherapy Integrated Methods - Part A, Volume 635 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry, High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers, Multiplex assay by IHC for melanoma tumor microenvironment evaluation, Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images, Phenotyping of immune cells in situ using multispectral imaging quantification, and much more.

Key Features

  • Authored by leaders in the field of enzymology
  • Provides a comprehensiveness level of discussion on the field
  • Presents a highly specialized group of topics that delve deep into new updates and future prospects


Experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques

Table of Contents

  • 1. High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers
    Grace Banik, Courtney B. Betts, Shannon M. Liudahl, Shamilene Sivagnanam, Rie Kawashima, Tiziana Cotechini, William Larson, Jeremy Goecks, Sara I. Pai, Daniel R. Clayburgh, Takahiro Tsujikawa and Lisa M. Coussens
    2. Multiplex immunohistochemistry assay to evaluate the melanoma tumor microenvironment
    Giosuè Scognamiglio, Mariaelena Capone, Domenico Mallardo, Gerardo Botti, Paolo A. Ascierto and Gabriele Madonna
    3. Characterization of the immune microenvironment of NSCLC by multispectral analysis of multiplex immunofluorescence images
    Michael Surace, Lorenz Rognoni, Jaime Rodriguez-Canales and Keith E. Steele
    4. Multiplexed immunohistochemistry for immune cell phenotyping, quantification and spatial distribution in situ
    Angela Vasaturo and Jérôme Galon
    5. Multiplex immunohistochemistry as a novel tool for the topographic assessment of the bone marrow stem cell niche
    Marcus Bauer, Christoforos Vaxevanis, Daniel Bethmann, Chiara Massa, Nikolaos Pazaitis, Claudia Wickenhauser and Barbara Seliger
    6. Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry
    Ileana S. Mauldin, Natasha D. Sheybani, Samuel J. Young, Richard J. Price and Craig L. Slingluff
    7. Tracing bone marrow-derived microglia in brain metastatic tumors
    Wei Du and Paula D. Bos
    8. Monitoring abscopal responses to radiation in mice
    Maria Esperanza Rodriguez-Ruiz, Takahiro Yamazaki, Aitziber Buqué, Norma Bloy, Viviane A. O. Silva, Lena Stafford, Ai Sato and Lorenzo Galluzzi
    9. Methods to assess anticancer immune responses in orthotopic bladder carcinomas
    Randy F. Sweis
    10. Characterization of conventional dendritic cell populations in preclinical tumor models using flow cytometry
    Erik Wennerberg, Nils Rudqvist and Sheila Spada
    11. Quantitative evaluation of tumor-specific T cells in tumors and lymphoid tissues
    Kathleen M. Kokolus, Nataša Obermajer and Pawel Kalinski
    12. Generation and validation of recombinant herpes simplex type 1 viruses (HSV-1) using CRISPR/Cas9 genetic disruption
    Praveen K. Bommareddy, Cole Peters and Howard L. Kaufman
    13. Production and use of adeno-associated virus vectors as tools for cancer immunotherapy
    Myriam Fernandez-Sendin, Shirley Tenesaca, Marcos Vasquez, Fernando Aranda and Pedro Berraondo
    14. Modulation of the tumor microenvironment with an oncolytic adenovirus for effective T-cell therapy and checkpoint inhibition
    João Manuel Santos, Riikka Havunen and Akseli Hemminki
    15. Genetic modification of oncolytic viruses to enhance antitumor immunity
    Maria Eugenia Davola, Alyssa Vito, Jiarun Wei, Nader El-Sayes, Samuel Workenhe and Karen Louise Mossman
    16. CRISPR technology for immuno-oncology applications
    Jianbin Wang, Vitaly Balan and Francesco Marincola
    17. Neoantigen prediction from genomic and transcriptomic data
    Sebastiano Battaglia
    18. Monitoring antigen cross-presentation with human dendritic cells purified from the blood
    Stephane Isnard, Etienne Hatton, Jean-Baptiste Guillerme and Anne Hosmalin

Product details

  • No. of pages: 344
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: February 28, 2020
  • Imprint: Academic Press
  • eBook ISBN: 9780128188750
  • Hardcover ISBN: 9780128186770
  • About the Serial Volume Editors

    Lorenzo Galluzzi

    Lorenzo Galluzzi
    Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

    Affiliations and Expertise

    Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

    Nils-Petter Rudqvist

    Nils-Petter Rudqvist
    Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

    Affiliations and Expertise

    Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA