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1. Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry
2. High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers
3. Multiplex assay by IHC for melanoma tumor microenviroment evaluation
4. Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images
5. Phenotyping of immune cells in situ using multispectral imaging quantification
6. Multiplex immunohistochemistry as a novel tool for the topographic assessment of the bone marrow stem cell niche
7. Immunoscore assay for the immune classification of solid tumors: technical aspects, improvements and clinical perspectives
8. Tissue transcriptome-based computation algorithms for determining immune cell compositions in tumor microenvironment
9. Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseq
10. DIY: Visualizing the immune landscape of tumors using transcriptome and methylome data
11. A comprehensive approach to gene expression profiling in immune cells
12. Using epigenetic data to estimate immune composition in admixed samples
13. Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancers
14. Immunohistochemistry for DAMP and autophagy detection
Tumor Immunology and Immunotherapy Integrated Methods - Part A, Volume 635 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry, High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers, Multiplex assay by IHC for melanoma tumor microenvironment evaluation, Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images, Phenotyping of immune cells in situ using multispectral imaging quantification, and much more.
- Authored by leaders in the field of enzymology
- Provides a comprehensiveness level of discussion on the field
- Presents a highly specialized group of topics that delve deep into new updates and future prospects
Experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques
- No. of pages:
- © Academic Press 2020
- 15th March 2020
- Academic Press
- Hardcover ISBN:
Lorenzo Galluzzi (born 1980) is currently Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College (New York, USA), and Honorary Associate Professor at the Faculty of Medicine of the Paris Descartes University (Paris, France). Prior to joining Weill Cornell Medical College (2017), Lorenzo Galluzzi was a Junior Scientist of the Research Team “Apoptosis, Cancer and Immunity” at the Cordeliers Research Center (Paris, France; 2012-2016). Lorenzo Galluzzi did his post-doctoral training at the Gustave Roussy Cancer Center (Villejuif, France; 2009-2011), after receiving his PhD from the Paris Sud University (Le Kremlin-Bicetre, France; 2005-2008). He is also Associate Director of the European Academy for Tumor Immunology (EATI), and Founding Member of the European Research Institute for Integrated Cellular Pathology (ERI-ICP). Lorenzo Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. In particular, he provided profound insights into the links between adaptive stress responses in cancer cells and the activation of a clinically relevant tumor-targeting immune response in the context of chemotherapy and radiation therapy. Lorenzo Galluzzi has published more than 350 scientific articles in international peer-reviewed journals. According to a survey published by Lab Times, he is currently the 6th and the youngest of the 30 most-cited European cell biologists (relative to the period 2007–2013). Lorenzo Galluzzi currently operates as Editor-in-Chief of three journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, and Molecular and Cellular Oncology (which he co-founded in 2013). In addition, Lorenzo Galluzzi currently serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease.
Weill Cornell Medical College, USA
Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.
Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA