Tumor Immunology and Immunotherapy – Cellular Methods Part A

1st Edition, Volume 631 - January 14, 2020
Editors: Lorenzo Galluzzi, Nils-Petter Rudqvist
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 1 8 6 7 3 - 2
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 1 8 6 7 4 - 9

Tumor Immunology and Immunotherapy – Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial w… Read more

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Tumor Immunology and Immunotherapy – Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more.

1. Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry
Jeremy B. Foote, Sujith Sarvesh and Leisha A. Emens

2. High-throughput identification of human antigen-specific CD8⁺ and CD4⁺ T cells using soluble pMHC multimers
Morgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt

3. In vitro assays for effector T cell functions and activity of immunomodulatory antibodies
Roberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub

4. A cytofluorimetric assay to evaluate T cell polyfunctionality
Belinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nisticò

5. Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells
Mathias Wenes, Pedro Romero and Lianjun Zhang

6. Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models
Mahmoud S. Alghamri, Felipe J. Núñez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro

7. Methods to edit T cells for cancer immunotherapy
Francesca Lucibello, Silvia Menegatti and Laurie Menger

8. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer
Karolina Pilipow, Eloise Scamardella and Enrico Lugli

9. Reverse immunology: From peptide sequence to tumor-killing human T-cell clones
Christophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger

10. Generation of TCR-engineered reference cell samples to control T-cell assay performance
Nicole Bidmon, Cécile Gouttefangeas and Sjoerd H. van der Burg

11. In vitro expansion of Vγ9Vδ2 T cells for immunotherapy
Christian Peters, Léonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz

12. CFSE dilution to study human T and NK cell proliferation in vitro
Iñigo Terrén, Ane Orrantia, Joana Vitallé, Olatz Zenarruzabeitia and Francisco Borrego

13. Rapid isolation and enrichment of mouse NK cells for experimental purposes
Maite Alvarez, Maria C. Ochoa, Luna Minute, Ignacio Melero and Pedro Berraondo

14. Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood
Peter Holicek, Iva Truxova, Lenka Kasikova, Sarka Vosahlikova, Cyril Salek, Jana Rakova, Monika Holubova, Daniel Lysak, Isabelle Cremer, Radek Spisek and Jitka Fucikova

15. Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells
Alexandra Frazao, Louise Rethacker and Anne Caignard

16. Rapid isolation of mouse ILCs from murine intestinal tissues
Kyle Burrows, Pailin Chiaranunt, Louis Ngai and Arthur Mortha

17. Detecting and analyzing murine innate lymphoid cells
Alejandra Gomez-Cadena, Pedro Romero, Sara Trabanelli and Camilla Jandus

18. A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells
Christian Sordo-Bahamonde, Seila Lorenzo-Herrero, Segundo González and Alejandro López-Soto

19. Applications of microfluidic devices in advancing NK-cell migration studies
Xiaoou Ren, Abdulaziz Alamri, Jolly Hipolito, Francis Lin and Sam K.P. Kung

20. Complementary approaches to study NKT cells in cancer
Jay A. Berzofsky, Purevdorj B. Olkhanud and Masaki Terabe

21. Assessment of IFN-γ and granzyme-B production by in "sitro" technology
Claudia Galassi, Gwenola Manic, Martina Musella, Antonella Sistigu and Ilio Vitale

22. Assessment of IFNγ responsiveness in patient-derived xenografts
Jordan J. Cardenas, Camila Robles-Oteiza and Katerina Politi

23. Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro
Hans-Heinrich Oberg, Christian Peters, Dieter Kabelitz and Daniela Wesch

24. Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attack
Natalie J. Neubert, Laure Tillé, Christophe Martignier, Silvia A. Fuertes Marraco and Daniel E. Speiser

25. A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity
Javier Santos, Jesús Ogando, Rosa Ana Lacalle and Santos Mañes

26. Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay
Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González and Alejandro López-Soto

27. 51Cr-release to monitor NK cell cytotoxicity
Leslie Elsner and Ralf Dressel

Lorenzo Galluzzi

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

Nils-Petter Rudqvist

Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

Affiliations and expertise

Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA