Tumor Immunology and Immunotherapy – Cellular Methods Part A - 1st Edition - ISBN: 9780128186732

Tumor Immunology and Immunotherapy – Cellular Methods Part A, Volume 631

1st Edition

Serial Volume Editors: Lorenzo Galluzzi Nils-Petter Rudqvist
Hardcover ISBN: 9780128186732
Imprint: Academic Press
Published Date: 24th January 2020
Page Count: 556
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Table of Contents

    1. Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry
    2. Jeremy B. Foote, Sujith Sarvesh and Leisha A. Emens

    3. High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers
    4. Morgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt

    5. In vitro assays for effector T cell functions and activity of immunomodulatory antibodies
    6. Roberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub

    7. A cytofluorimetric assay to evaluate T cell polyfunctionality
    8. Belinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nisticò

    9. Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells
    10. Mathias Wenes, Pedro Romero and Lianjun Zhang

    11. Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models
    12. Mahmoud S. Alghamri, Felipe J. Núñez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro

    13. Methods to edit T cells for cancer immunotherapy
    14. Francesca Lucibello, Silvia Menegatti and Laurie Menger

    15. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer
    16. Karolina Pilipow, Eloise Scamardella and Enrico Lugli

    17. Reverse immunology: From peptide sequence to tumor-killing human T-cell clones
    18. Christophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger

    19. Generation of TCR-engineered reference cell samples to control T-cell assay performance
    20. Nicole Bidmon, Cécile Gouttefangeas and Sjoerd H. van der Burg



    21. In vitro expansion of Vγ9Vδ2 T cells for immunotherapy
    22. Christian Peters, Léonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz

    23. CFSE dilution to study human T and NK cell proliferation in vitro
    24. Iñigo Terrén, Ane Orrantia, Joana Vitallé, Olatz Zenarruzabeitia and Francisco Borrego

    25. Rapid isolation and enrichment of mouse NK cells for experimental purposes
    26. Maite Alvarez, Maria C. Ochoa, Luna Minute, Ignacio Melero and Pedro Berraondo

    27. Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood
    28. Peter Holicek, Iva Truxova, Lenka Kasikova, Sarka Vosahlikova, Cyril Salek, Jana Rakova, Monika Holubova, Daniel Lysak, Isabelle Cremer, Radek Spisek and Jitka Fucikova

    29. Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells
    30. Alexandra Frazao, Louise Rethacker and Anne Caignard

    31. Rapid isolation of mouse ILCs from murine intestinal tissues
    32. Kyle Burrows, Pailin Chiaranunt, Louis Ngai and Arthur Mortha

    33. Detecting and analyzing murine innate lymphoid cells
    34. Alejandra Gomez-Cadena, Pedro Romero, Sara Trabanelli and Camilla Jandus

    35. A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells
    36. Christian Sordo-Bahamonde, Seila Lorenzo-Herrero, Segundo González and Alejandro López-Soto

    37. Applications of microfluidic devices in advancing NK-cell migration studies
    38. Xiaoou Ren, Abdulaziz Alamri, Jolly Hipolito, Francis Lin and Sam K.P. Kung

    39. Complementary approaches to study NKT cells in cancer
    40. Jay A. Berzofsky, Purevdorj B. Olkhanud and Masaki Terabe

    41. Assessment of IFN-γ and granzyme-B production by in "sitro" technology
    42. Claudia Galassi, Gwenola Manic, Martina Musella, Antonella Sistigu and Ilio Vitale

    43. Assessment of IFNγ responsiveness in patient-derived xenografts
    44. Jordan J. Cardenas, Camila Robles-Oteiza and Katerina Politi

    45. Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro
    46. Hans-Heinrich Oberg, Christian Peters, Dieter Kabelitz and Daniela Wesch

    47. Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attack
    48. Natalie J. Neubert, Laure Tillé, Christophe Martignier, Silvia A. Fuertes Marraco and Daniel E. Speiser

    49. A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity
    50. Javier Santos, Jesús Ogando, Rosa Ana Lacalle and Santos Mañes

    51. Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay
    52. Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González and Alejandro López-Soto

    53. 51Cr-release to monitor NK cell cytotoxicity

Leslie Elsner and Ralf Dressel


Tumor Immunology and Immunotherapy – Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more.

Key Features

  • Contains the authority of authors who are leaders in their field
  • Provides a comprehensive source on new methods and research in enzymology


Experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques


No. of pages:
© Academic Press 2020
24th January 2020
Academic Press
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editors

Lorenzo Galluzzi

Lorenzo Galluzzi Serial Volume Editor

Lorenzo Galluzzi (born 1980) is currently Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College (New York, USA), and Honorary Associate Professor at the Faculty of Medicine of the Paris Descartes University (Paris, France). Prior to joining Weill Cornell Medical College (2017), Lorenzo Galluzzi was a Junior Scientist of the Research Team “Apoptosis, Cancer and Immunity” at the Cordeliers Research Center (Paris, France; 2012-2016). Lorenzo Galluzzi did his post-doctoral training at the Gustave Roussy Cancer Center (Villejuif, France; 2009-2011), after receiving his PhD from the Paris Sud University (Le Kremlin-Bicetre, France; 2005-2008). He is also Associate Director of the European Academy for Tumor Immunology (EATI), and Founding Member of the European Research Institute for Integrated Cellular Pathology (ERI-ICP). Lorenzo Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. In particular, he provided profound insights into the links between adaptive stress responses in cancer cells and the activation of a clinically relevant tumor-targeting immune response in the context of chemotherapy and radiation therapy. Lorenzo Galluzzi has published more than 350 scientific articles in international peer-reviewed journals. According to a survey published by Lab Times, he is currently the 6th and the youngest of the 30 most-cited European cell biologists (relative to the period 2007–2013). Lorenzo Galluzzi currently operates as Editor-in-Chief of three journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, and Molecular and Cellular Oncology (which he co-founded in 2013). In addition, Lorenzo Galluzzi currently serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease.

Affiliations and Expertise

Weill Cornell Medical College, USA

Nils-Petter Rudqvist

Nils-Petter Rudqvist Serial Volume Editor

Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

Affiliations and Expertise

Postdoctoral Associate in Radiation Oncology, Weill Cornell Medicine, USA