Translation Initiation: Reconstituted Systems and Biophysical Methods, Volume 430

Chapter 1 Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in Bacteria Pohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. Rodnina

Chapter 2 Binding of mRNA to the Bacterial Translation Initiation Complex Sean M. Studer and Simpson Joseph

Chapter 3 Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. Pon

Chapter 4 Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3 Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. Spremulli

Chapter 5 In Vitro Studies of Archaeal Translational Initiation Dario Benelli and Paola Londei

Chapter 6 Reconstitution of yeast translation initiation Michael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. Lorsch

Chapter 7 Assembly and analysis of eukaryotic translation initiation complexes Andrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. Pestova

Chapter 8 Reconstitution of Mammalian 48S Ribosomal Translation Initiation Complexes Romit Majumdar, Jayanta Chaudhuri and Umadas Maitra Chapter 9 Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analogues Anna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard Stolarski

Chapter 10 Biophysical studies of the translation initiation pathway using immobilised mRNA analogues John E.G. McCarthy, Steven Marsden and Tobias von der Haar

Chapter 11 Protection-Based Assays to Measure Amino Acyl-tRNA Binding to Translation Initiation Factors Yves Mechulam*, Laurent Guillon, Laure Yatime, Sylvain Blanquet and Emmanuelle Schmitt

Chapter 12 NMR methods for studying protein-protein interactions involved in translation initiation Assen Marintchev, Dominique Frueh, and Gerhard Wagnerƒx

Chapter 13 Structural methods to study IRES Function Jeffrey S. Kieft*, David A. Costantino, Megan E. Filbin, John Hammond, and Jennifer S. Pfingsten

Chapter 14 Biophysical and biochemical investigations of dsRNA-activated kinase PKR. Sean A. McKenna, Darrin A. Lindhout, Takashi Shimoike, and Joseph D. Puglisi

Chapter 15 Expression and purification of recombinant wheat translation initiation factors eIF1, eIF1A, eIF4A, eIF4B, eIF4F, eIF(iso)4F and eIF5 Laura K. Mayberry, Michael D. Dennis, M. Leah Allen, Kelley Ruud Nitka, Patricia A. Murphy, Lara Campbell and Karen S. Browning

Chapter 16 In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entry Victoria G. Kolupaeva, Sylvain de Breyne, Tatyana V. Pestova, Christopher U.T. Hellen

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.

Biochemists and researchers in related life science disciplines.