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Chapter 1 Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in Bacteria Pohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. Rodnina
Chapter 2 Binding of mRNA to the Bacterial Translation Initiation Complex Sean M. Studer and Simpson Joseph
Chapter 3 Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. Pon
Chapter 4 Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3 Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. Spremulli
Chapter 5 In Vitro Studies of Archaeal Translational Initiation Dario Benelli and Paola Londei
Chapter 6 Reconstitution of yeast translation initiation Michael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. Lorsch
Chapter 7 Assembly and analysis of eukaryotic translation initiation complexes Andrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. Pestova
Chapter 8 Reconstitution of Mammalian 48S Ribosomal Translation Initiation Complexes Romit Majumdar, Jayanta Chaudhuri and Umadas Maitra Chapter 9 Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analogues Anna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard Stolarski
Chapter 10 Biophysical studies of the translation initiation pathway using immobilised mRNA analogues John E.G. McCarthy, Steven Marsden and Tobias von der Haar
Chapter 11 Protection-Based Assays to Measure Amino Acyl-tRNA Binding to Translation Initiation Factors Yves Mechulam*, Laurent Guillon, Laure Yatime, Sylvain Blanquet and Emmanuelle Schmitt
Chapter 12 NMR methods for studying protein-protein interactions involved in translation initiation Assen Marintchev, Dominique Frueh, and Gerhard Wagnerƒx
Chapter 13 Structural methods to study IRES Function Jeffrey S. Kieft*, David A. Costantino, Megan E. Filbin, John Hammond, and Jennifer S. Pfingsten
Chapter 14 Biophysical and biochemical investigations of dsRNA-activated kinase PKR. Sean A. McKenna, Darrin A. Lindhout, Takashi Shimoike, and Joseph D. Puglisi
Chapter 15 Expression and purification of recombinant wheat translation initiation factors eIF1, eIF1A, eIF4A, eIF4B, eIF4F, eIF(iso)4F and eIF5 Laura K. Mayberry, Michael D. Dennis, M. Leah Allen, Kelley Ruud Nitka, Patricia A. Murphy, Lara Campbell and Karen S. Browning
Chapter 16 In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entry Victoria G. Kolupaeva, Sylvain de Breyne, Tatyana V. Pestova, Christopher U.T. Hellen
For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.
Biochemists and researchers in related life science disciplines.
- No. of pages:
- © Academic Press 2007
- 9th October 2007
- Academic Press
- Hardcover ISBN:
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