For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the second of three on the topic of Translation Initiation includes articles written by leaders in the field.


Biochemists and researchers in related life science disciplines.

Table of Contents

Chapter 1 Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in Bacteria Pohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. Rodnina Chapter 2 Binding of mRNA to the Bacterial Translation Initiation Complex Sean M. Studer and Simpson Joseph Chapter 3 Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. Pon Chapter 4 Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3 Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. Spremulli Chapter 5 In Vitro Studies of Archaeal Translational Initiation Dario Benelli and Paola Londei Chapter 6 Reconstitution of yeast translation initiation Michael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. Lorsch Chapter 7 Assembly and analysis of eukaryotic translation initiation complexes Andrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. Pestova Chapter 8 Reconstitution of Mammalian 48S Ribosomal Translation Initiation Complexes Romit Majumdar, Jayanta Chaudhuri and Umadas Maitra Chapter 9 Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analogues Anna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard Stolarski Chapter 10 Biophysical studies of the translation initiation pathway u


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