Volume 430. Translation Initiation: Reconstituted Systems and Biophysical Methods

Chapter 1 Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in Bacteria Pohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. Rodnina Chapter 2 Binding of mRNA to the Bacterial Translation Initiation Complex Sean M. Studer and Simpson Joseph Chapter 3 Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. Pon Chapter 4 Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3 Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. Spremulli Chapter 5 In Vitro Studies of Archaeal Translational Initiation Dario Benelli and Paola Londei Chapter 6 Reconstitution of yeast translation initiation Michael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. Lorsch Chapter 7 Assembly and analysis of eukaryotic translation initiation complexes Andrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. Pestova Chapter 8 Reconstitution of Mammalian 48S Ribosomal Translation Initiation Complexes Romit Majumdar, Jayanta Chaudhuri and Umadas Maitra Chapter 9 Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analogues Anna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard Stolarski Chapter 10 Biophysical studies of the translation initiation pathway u