Translation Initiation: Cell Biology, High-throughput and Chemical-based Approaches

Translation Initiation: Cell Biology, High-throughput and Chemical-based Approaches

1st Edition - October 12, 2007

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  • Editor: Jon Lorsch
  • eBook ISBN: 9780080553481
  • Hardcover ISBN: 9780123739643

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For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the third of three on the topic of Translation Initiation includes articles written by leaders in the field.


Biochemists and researchers in related life science disciplines.

Table of Contents

  • Chapter 1
    In vivo deletion analysis of the architecture of a multi-protein complex of translation initiation factors.
    Klaus H. Nielsen and Leoš Valášek

    Chapter 2
    An approach to studying the localization and dynamics of eukarayotic translation factors in live yeast cells
    Susan G. Campbell and Mark P Ashe

    Chapter 3
    In Vitro and Tissue Culture Methods for Analysis of Translation Initiation on the Endoplasmic Reticulum
    Samuel B. Stephens and Christopher V. Nicchitti

    Chapter 4
    Mammalian Stress Granules and Processing Bodies
    Nancy Kedersha and Paul Anderson

    Chapter 5
    Methods to Analyse MircroRNA-meciated control of mRNA translation
    Jennifer L. Clancy, Marco Nousch, David T. Humphreys, Belinda J. Westman, Traude H. Beilharz, Thomas Preiss

    Chapter 6
    Methods for studying signal-dependent regulation of translation factor activity and its role in disease
    Christopher Proud

    Chapter 7
    Analysis of mRNA Translation in Cultured Hippocampal Neurons
    Yi-Shuian Huang and Joel D. Richter

    Chapter 8
    Detecting ribosomal association with the 5' leader of mRNAs by Ribosome Density Mapping (RDM)
    Naama Eldad and Yoav Arava

    Chapter 9
    Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarrays
    Daniel Melamed and Yoav Arava

    Chapter 10
    Synthesis of Anti-Reverse Cap Analogs (ARCAs) and Their Applications in mRNA Translation and Stability
    Ewa Grudzien-Nogalska, Janusz Stepinski, Jacek Jemielity, Joanna Zuberek, Ryszard Stolarski, Robert E. Rhoads, Edward Darzynkiewicz*

    Chapter 11
    Methods for identifying compounds that specifically target translation
    Letizia Brandi, Attilio Fabbretti, Pohl Milon, Marcello Carotti, Cynthia L. Pon, Claudio O. Gualerzi

    Chapter 12
    Identifying Small Molecule Inhibitors of Eukaryotic Translation Initiation
    Regina Cencic, Francis Robert, Jerry Pelletier

    Chapter 13
    Identification of the targets of drugs affecting eukaryotic translation initiation
    Jun Liu

    Chapter 14
    Purification of FLAG-tagged Eukaryotic Initiation Factor 2B Complexes, Sub-complexes and Fragments from Saccharomyces cerevisiae
    Sarah S. Mohammad-Qureshi, Raphael Haddad, Karen S. Palmer, Jonathan P. Richardson, Edith Gomez, Graham D. Pavitt

Product details

  • No. of pages: 392
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: October 12, 2007
  • Imprint: Academic Press
  • eBook ISBN: 9780080553481
  • Hardcover ISBN: 9780123739643

About the Serial Volume Editor

Jon Lorsch

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