Transgenic Animal Technology

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students.

• Contains a comprehensive collection of transgenic animal and gene transfer methods
• Discusses background and introduction to techniques and animal systems
• Teaches practical step-by-step protocols
• Describes mouse model systems
• Covers laboratory and domestic animal species
• Illustrated with diagrams and photographs
• Includes useful tables and charts

Researchers, students, technicians, and administrators in academic, government, and industrial laboratories in life science, biomedical science, agricultural science, and biotechnology

Overview:

C.A. Pinkert, Introduction to Transgenic Animals.

Transgenic Animal Production Focusing on the Mouse Model:

H.G. Polites and C.A. Pinkert, Transgenic Animal Production Using DNA Microinjection.

P. Overbeek, Factors Affecting Transgenic Animal Production.

T. Doetschman, Gene Transfer in Embryonic Stem Cells.

P.A. Wood, Retrovirus-Mediated Gene Transfer.

G.M. Monastersky, Gene Transfer Technology: Alternative Techniques and Applications.

B.T. Tinkle, C.J. Bieberich, and G. Jay, Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Integration.

C.J. Bieberich, L. Ngo, and G. Jay, Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Expression.

Production of Transgenic Laboratory and Domestic Animal Species:

J.M. Robl and J.K. Heideman, Production of Transgenic Rabbits and Rats.

L. Cioffi, J.J. Kopchik, and H.Y. Chen, Production of TransgenicPoultry and Fish.

M.J. Martin and C.A. Pinkert, Production of Transgenic Swine.

C.E. Rexroad, Jr. and H.W. Hawk, Production of Transgenic Ruminants.