Description

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students.

Key Features

@introbul:Key Features @bul:* Contains a comprehensive collection of transgenic animal and gene transfer methods * Discusses background and introduction to techniques and animal systems * Teaches practical step-by-step protocols * Describes mouse model systems * Covers laboratory and domestic animal species, including: @subbul:* Rabbits and rats * Poultry and fish * Swine and ruminants @bul:* Illustrated with diagrams and photographs * Includes useful tables and charts

Readership

Researchers, students, technicians, and administrators in academic, government, and industrial laboratories in life science, biomedical science, agricultural science, and biotechnology.

Table of Contents

Overview: C.A. Pinkert, Introduction to Transgenic Animals. Transgenic Animal Production Focusing on the Mouse Model: H.G. Polites and C.A. Pinkert, Transgenic Animal Production Using DNA Microinjection. P. Overbeek, Factors Affecting Transgenic Animal Production. T. Doetschman, Gene Transfer in Embryonic Stem Cells. P.A. Wood, Retrovirus-Mediated Gene Transfer. G.M. Monastersky, Gene Transfer Technology: Alternative Techniques and Applications. B.T. Tinkle, C.J. Bieberich, and G. Jay, Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Integration. C.J. Bieberich, L. Ngo, and G. Jay, Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Expression. Production of Transgenic Laboratory and Domestic Animal Species: J.M. Robl and J.K. Heideman, Production of Transgenic Rabbits and Rats. L. Cioffi, J.J. Kopchik, and H.Y. Chen, Production of TransgenicPoultry and Fish. M.J. Martin and C.A. Pinkert, Production of Transgenic Swine. C.E. Rexroad, Jr. and H.W. Hawk, Production of Transgenic Ruminants.

Details

No. of pages:
364
Language:
English
Copyright:
© 1994
Published:
Imprint:
Academic Press
Print ISBN:
9780125571654
Electronic ISBN:
9780323137836

About the author

Carl Pinkert

Dr. Pinkert joined the University of Alabama (UA) in 2013 where he serves as Vice President for Research and Economic Development, and where he is a tenured Professor in the Department of Biological Sciences in the College of Arts and Sciences. He earned his Ph.D. from the University of Georgia and was a postdoctoral fellow at the University of Pennsylvania. For over 30 years, his research revolved around animal modeling and transgenesis. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. His laboratory developed a number of enabling technologies for the genetic engineering of both nuclear and mitochondrial genomes, as well as mitochondrial modeling strategies to address human developmental and metabolic diseases. He served as an editor of Transgenic Research on the editorial boards of five others. His research was funded by NIH, USDA, NSF, private foundations and industry. He was a Sigma Xi national lecturer from 1993-1995 and a recipient of the 1997 Doerenkamp-Zbinden Foundation Research Prize (awarded for biomedical research that significantly impacts on the welfare and well-being of animals used in research). He was elected a fellow of the American Association for the Advancement of Science (AAAS) in 2011.

Reviews

@qu:"First and foremost, this is a book for everyone interested in technology. The contributing authors have gone to great lengths to make the methodology clear and understandable to anyone with a working knowledge of molecularbiology and embryology. Each chapter is well-illustrated with diagrams and photographs and is accompanied by an extensive bibliography. Potential problems and possible solutions are discussed in detail. The second unique aspect of this book is its state of the art coverage of transgenesis in vertebrate animal species other than mice... Each chapter is well integrated with the others and is sufficiently detailed to be useful. The editor and contributing authors are to be congratulated for putting this information together in an easy accessible format which is sure to stimulate further interest and development in the field." @source:--THERIOGENOLOGY @qu:"Transgenic Animal Technology is a laboratory manual that documents detailed methods required toaccomplish the gene transfers necessary to produce animals with altered genetic characteristics." @source:--JOURNAL OF THE AMERICAN VETERINARY ASSOCIATION