- Contains a comprehensive collection of transgenic animal and gene transfer methods
- Discusses background and introduction to techniques and animal systems
- Teaches practical step-by-step protocols
- Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Students, postdoctoral fellows, and researchers at academic and industrial/agricultural laboratories using non-traditional transgenic models in such areas as molecular and cell biology, developmental biology, neuroscience, pharmacology, biophysiology, cardiology, oncology and genetics.
Table of Contents
Introduction to transgenic animals.
Transgenic animal production focusing on the mouse model.
Transgenic animal production using DNA microinjection.
Factors affecting transgenic animal production.
Gene targeting in embryonic stem cells I: History and methodology.
Gene targeting in embryonic stem cells II. Conditional technologies.
Retrovirus-mediated gene transfer.
Nuclear transfer technologies.
Production of transgenic laboratory and domestic animal species.
Production of transgenic rats.
Production of transgenic rabbits.
Production of transgenic fish.
Production of transgenic poultry.
Production of transgenic swine by DNA Microinjection.
Production of transgenic ruminants by DNA Microinjection.
Production of transgenic non-human primates.
Retrovirus-mediated gene transfer into oocytes.
Section Four: Molecular biology, analyses and enabling technologies
Vector design for transgene expression.
Analysis of transgene integration.
PCR optimization for detection of transgenic integration.
Evaluation of transgene expression.
Control of gene expression (from standard gain/loss of function to anti-sense to PNAs).
Enabling technologies: Cryopreservation of gametes.
Enabling technologies: Embryo culture considerations.
Databases, Internet Resources and Genetic Nomenclature.
- No. of pages: 714
- Language: English
- Copyright: © Elsevier 2014
- Published: June 13, 2014
- Imprint: Elsevier
- Hardcover ISBN: 9780124104907
- eBook ISBN: 9780124095366
About the Author
He earned his Ph.D. from the University of Georgia and was a postdoctoral fellow at the University of Pennsylvania. For over 30 years, his research revolved around animal modeling and transgenesis. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. His laboratory developed a number of enabling technologies for the genetic engineering of both nuclear and mitochondrial genomes, as well as mitochondrial modeling strategies to address human developmental and metabolic diseases.
He served as an editor of Transgenic Research on the editorial boards of five others. His research was funded by NIH, USDA, NSF, private foundations and industry. He was a Sigma Xi national lecturer from 1993-1995 and a recipient of the 1997 Doerenkamp-Zbinden Foundation Research Prize (awarded for biomedical research that significantly impacts on the welfare and well-being of animals used in research). He was elected a fellow of the American Association for the Advancement of Science (AAAS) in 2011.
Affiliations and Expertise
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