Transfer and Expression of Eukaryotic Genes - 1st Edition - ISBN: 9780122846502, 9780323138659

Transfer and Expression of Eukaryotic Genes

1st Edition

Authors: H.S. Ginsberg
eBook ISBN: 9780323138659
Imprint: Academic Press
Published Date: 28th January 1984
Page Count: 304
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Description

Transfer and Expression of Eukaryotic Genes documents the progress in our understanding of the transfer and expression of eukaryotic genes. This book covers topics organized around three themes: gene expression and its regulation; in vivo gene transfer and development; and viral gene and oncogene systems. This text is divided into three sections encompassing 25 chapters and begins with an overview of the molecular basis of gene expression, with emphasis on transcription complexes that account for transcription control in eukaryotic genes. It then turns to experiments that assess the in vitro stimulatory effect of the SV40 72-bp repeat on specific transcription from heterologous promoter elements using a HeLa whole cell extract. The reader is methodically introduced to the regulation signals and factors of histone gene transcription; transcriptional control of beta-globin and liver-specific genes in mouse cells; and gene transfer in Drosophila and the sea urchin Strongylocentrotus purpuratus. This book also considers the splicing of messenger RNA precursors and the regulation of thymidine kinase enzyme expression, and then concludes with a chapter that describes the activation of the myc oncogene by chromosomal translocation. This book will be of interest to students and researchers in fields ranging from molecular genetics to microbiology, biochemistry, pathology, and immunology.

Table of Contents


List of Participants

Sol Spiegelman (1914-1983)

Preface

Opening Address

On the Molecular Basis of Gene Expression

Text

Part I. Gene Expression and Its Regulation

Stimulation of In Vitro Transcription from Heterologous Promoters by the SV40 Enhancer

Introduction

Materials and Methods

Results

Discussion

References

Correct Developmental and Tissue Specific Expression of a Cloned Alcohol Dehydrogenase Gene Introduced into the Drosophila Germ Line by P Element Transformation

Introduction

Results

Discussion

Conclusion

References

DNA Methylation and Globin Gene Expression

Introduction

Results

Summary

References

Regulation Signals and Factors of Histone Gene Transcription

Developmental Regulation of Sea Urchin Histone Gene Expression

Structure and Function of Histone Gene Promoters

Regulatory Factors Involved in Initiation of Histone Gene Transcription

DNA Signals Responsible for the Generation of Histone mRNA 3' Termini

Regulatory Factors Involved in 3' Processing of Histone Gene Transcripts

References

Transcriptional Control of β-Globin and Liver-Specific Genes in Mouse Cells

Introduction

Methods of Study of Transcription in Eukaryotic Cells

Transcription of the Mouse β-Globin Gene: Evidence for a Termination Signal

Induced Differential Transcription and Chromatin Rearrangement in the β-Globin Region

Hepatocyte-Specific Gene Control

References

Transcriptional Enhancement by Specific Regulatory Protein-DNA Complexes

Introduction

Receptor Binds Specifically In Vitro to MTV DNA Upstream of the Transcription Start Site

In Vitro Receptor Binding Region within the MTV LTR is a Receptor-Dependent Enhancer In Vivo

Additional Receptor Binding Sites Reside within Transcribed MTV DNA Sequences

Perspectives and Speculation

References

Control Elements Located within the Major Intervening Sequences of Murine Kappa Light Chain Genes

Introduction

Results

Discussion

References

Part II. In Vivo Gene Transfer and Development

The Use of Transposable Elements as Vectors for Gene Transfer in Drosophila

Introduction

P Element-Mediated Gene Transfer

Expression of Transduced rosy Genes

Expression of Transduced white Genes

References

Gene Transfer in the Sea Urchin Strongylocentrotus purpuratus

Introduction

Discussion

References

Position Effects and Gene Expression in the Transgenic Mouse

Introduction

Results

Discussion

References

The Transfer of New Genes into Mice

Text

References

Isolation of Human Surface Receptor Genes by Eukaryotic Gene Transfer

Text

References

Nuclear Transplantation and Gene Transfer in Amphibian Eggs and Oocytes

Introduction

A Single Somatic Nucleus Transplanted to an Enucleated Egg

Multiple Nuclei in Oocytes

Purified Genes Injected into Fertilized Eggs

5 S Genes

Prospects

References

The Expression of Neuropeptide Genes in Developing and Adult Neurons

Introduction

A Gene Family Encodes the Egg-Laying Peptides

A System of ELH-Containing Neurons in the Adult

Development of the Bag Cells and Other Neurons That Express ELH

Conclusions

Summary

References

Partially Homologous Recombination in Mouse L Cells

Introduction

Results

Discussion

References

Part III. Viral Gene and Oncogene Systems

Splicing of Messenger RNA Precursors

Introduction

Current Experiments on the Biochemistry of mRNA Precursor Splicing

Conclusions

References

Studies on the Regulation of Thymidine Kinase Enzyme Expression

Introduction

Regulation of Viral tk Enzyme Expression

Regulation of Cellular tk Enzyme Expression

References

The Regulation of α Genes of Herpes Simplex Virus 1

Introduction

Evidence That α Genes Contain Inducible Regulatory Regions

The Regulatory Region of α Genes Can Be Moved Independently of the Promotor Region

The Regulatory Region of α Genes Consists of Multiple Elements

Discussion

References

Cis and Trans Acting Regulation of Early Adenovirus Transcription

Introduction

Results and Discussion

References

Mutational Analysis of the Functional Domains of the Ad5-E1a Gene Products Effecting Cell Transformation

Introduction

Conclusion

References

Adenovirus VAI RNA: A Virus-Coded Translational Enhancer

Introduction

Results and Discussion

Summary

References

Regulation of SV40 Early Transcription

Introduction

Fractionation of the Whole-Cell Extract

Transcription of 5’ Deletion Mutants

Transcription of Related Papovaviruses

Discussion

References

Transforming Genes of Chicken and Human B Cell Lymphomas

Introduction

Molecular Cloning and Analysis of a Transforming Gene from Chicken B Cell Lymphomas

Identification of Human Blym-1 as the Transforming Gene Detected by Transfection of Burkitt’s Lymphoma DNA

Role of Transforming Genes in Lymphomagenesis

References

Activation of the myc Oncogene by Chromosomal Translocation

Introduction

Materials and Methods

Results and Discussion

Conclusion

References

Index




Details

No. of pages:
304
Language:
English
Copyright:
© Academic Press 1984
Published:
Imprint:
Academic Press
eBook ISBN:
9780323138659

About the Author

H.S. Ginsberg