Transfer and Expression of Eukaryotic Genes
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Transfer and Expression of Eukaryotic Genes documents the progress in our understanding of the transfer and expression of eukaryotic genes. This book covers topics organized around three themes: gene expression and its regulation; in vivo gene transfer and development; and viral gene and oncogene systems. This text is divided into three sections encompassing 25 chapters and begins with an overview of the molecular basis of gene expression, with emphasis on transcription complexes that account for transcription control in eukaryotic genes. It then turns to experiments that assess the in vitro stimulatory effect of the SV40 72-bp repeat on specific transcription from heterologous promoter elements using a HeLa whole cell extract. The reader is methodically introduced to the regulation signals and factors of histone gene transcription; transcriptional control of beta-globin and liver-specific genes in mouse cells; and gene transfer in Drosophila and the sea urchin Strongylocentrotus purpuratus. This book also considers the splicing of messenger RNA precursors and the regulation of thymidine kinase enzyme expression, and then concludes with a chapter that describes the activation of the myc oncogene by chromosomal translocation. This book will be of interest to students and researchers in fields ranging from molecular genetics to microbiology, biochemistry, pathology, and immunology.
Table of Contents
List of Participants
Sol Spiegelman (1914-1983)
Preface
Opening Address
On the Molecular Basis of Gene Expression
Text
Part I. Gene Expression and Its Regulation
Stimulation of In Vitro Transcription from Heterologous Promoters by the SV40 Enhancer
Introduction
Materials and Methods
Results
Discussion
References
Correct Developmental and Tissue Specific Expression of a Cloned Alcohol Dehydrogenase Gene Introduced into the Drosophila Germ Line by P Element Transformation
Introduction
Results
Discussion
Conclusion
References
DNA Methylation and Globin Gene Expression
Introduction
Results
Summary
References
Regulation Signals and Factors of Histone Gene Transcription
Developmental Regulation of Sea Urchin Histone Gene Expression
Structure and Function of Histone Gene Promoters
Regulatory Factors Involved in Initiation of Histone Gene Transcription
DNA Signals Responsible for the Generation of Histone mRNA 3' Termini
Regulatory Factors Involved in 3' Processing of Histone Gene Transcripts
References
Transcriptional Control of β-Globin and Liver-Specific Genes in Mouse Cells
Introduction
Methods of Study of Transcription in Eukaryotic Cells
Transcription of the Mouse β-Globin Gene: Evidence for a Termination Signal
Induced Differential Transcription and Chromatin Rearrangement in the β-Globin Region
Hepatocyte-Specific Gene Control
References
Transcriptional Enhancement by Specific Regulatory Protein-DNA Complexes
Introduction
Receptor Binds Specifically In Vitro to MTV DNA Upstream of the Transcription Start Site
In Vitro Receptor Binding Region within the MTV LTR is a Receptor-Dependent Enhancer In Vivo
Additional Receptor Binding Sites Reside within Transcribed MTV DNA Sequences
Perspectives and Speculation
References
Control Elements Located within the Major Intervening Sequences of Murine Kappa Light Chain Genes
Introduction
Results
Discussion
References
Part II. In Vivo Gene Transfer and Development
The Use of Transposable Elements as Vectors for Gene Transfer in Drosophila
Introduction
P Element-Mediated Gene Transfer
Expression of Transduced rosy Genes
Expression of Transduced white Genes
References
Gene Transfer in the Sea Urchin Strongylocentrotus purpuratus
Introduction
Discussion
References
Position Effects and Gene Expression in the Transgenic Mouse
Introduction
Results
Discussion
References
The Transfer of New Genes into Mice
Text
References
Isolation of Human Surface Receptor Genes by Eukaryotic Gene Transfer
Text
References
Nuclear Transplantation and Gene Transfer in Amphibian Eggs and Oocytes
Introduction
A Single Somatic Nucleus Transplanted to an Enucleated Egg
Multiple Nuclei in Oocytes
Purified Genes Injected into Fertilized Eggs
5 S Genes
Prospects
References
The Expression of Neuropeptide Genes in Developing and Adult Neurons
Introduction
A Gene Family Encodes the Egg-Laying Peptides
A System of ELH-Containing Neurons in the Adult
Development of the Bag Cells and Other Neurons That Express ELH
Conclusions
Summary
References
Partially Homologous Recombination in Mouse L Cells
Introduction
Results
Discussion
References
Part III. Viral Gene and Oncogene Systems
Splicing of Messenger RNA Precursors
Introduction
Current Experiments on the Biochemistry of mRNA Precursor Splicing
Conclusions
References
Studies on the Regulation of Thymidine Kinase Enzyme Expression
Introduction
Regulation of Viral tk Enzyme Expression
Regulation of Cellular tk Enzyme Expression
References
The Regulation of α Genes of Herpes Simplex Virus 1
Introduction
Evidence That α Genes Contain Inducible Regulatory Regions
The Regulatory Region of α Genes Can Be Moved Independently of the Promotor Region
The Regulatory Region of α Genes Consists of Multiple Elements
Discussion
References
Cis and Trans Acting Regulation of Early Adenovirus Transcription
Introduction
Results and Discussion
References
Mutational Analysis of the Functional Domains of the Ad5-E1a Gene Products Effecting Cell Transformation
Introduction
Conclusion
References
Adenovirus VAI RNA: A Virus-Coded Translational Enhancer
Introduction
Results and Discussion
Summary
References
Regulation of SV40 Early Transcription
Introduction
Fractionation of the Whole-Cell Extract
Transcription of 5’ Deletion Mutants
Transcription of Related Papovaviruses
Discussion
References
Transforming Genes of Chicken and Human B Cell Lymphomas
Introduction
Molecular Cloning and Analysis of a Transforming Gene from Chicken B Cell Lymphomas
Identification of Human Blym-1 as the Transforming Gene Detected by Transfection of Burkitt’s Lymphoma DNA
Role of Transforming Genes in Lymphomagenesis
References
Activation of the myc Oncogene by Chromosomal Translocation
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
Index
Product details
- No. of pages: 304
- Language: English
- Copyright: © Academic Press 1984
- Published: January 28, 1984
- Imprint: Academic Press
- eBook ISBN: 9780323138659
About the Author
H.S. Ginsberg
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