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Tissue Engineering
2nd Edition - December 10, 2014
Authors: Clemens van Blitterswijk, Jan De Boer
Language: English
Hardback ISBN:9780124201453
9 7 8 - 0 - 1 2 - 4 2 0 1 4 5 - 3
eBook ISBN:9780124202108
9 7 8 - 0 - 1 2 - 4 2 0 2 1 0 - 8
Tissue Engineering is a comprehensive introduction to the engineering and biological aspects of this critical subject. With contributions from internationally renowned authors…Read more
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Tissue Engineering isa comprehensive introduction to the engineering and biological aspects of this critical subject. With contributions from internationally renowned authors, it provides a broad perspective on tissue engineering for students coming to the subject for the first time. In addition to the key topics covered in the previous edition, this update also includes new material on the regulatory authorities, commercial considerations as well as new chapters on microfabrication, materiomics and cell/biomaterial interface.
Effectively reviews major foundational topics in tissue engineering in a clear and accessible fashion
Includes state of the art experiments presented in break-out boxes, chapter objectives, chapter summaries, and multiple choice questions to aid learning
New edition contains material on regulatory authorities and commercial considerations in tissue engineering
Biomedical and tissue engineers; researchers; scientists; students
Preface
Chapter 1. Tissue Engineering: An Introduction
Chapter 2. Stem Cells
Learning Objectives
2.1. Introduction
2.2. Differentiation
2.3. Characterization of Stem Cells: Surface Protein Expression
2.4. Characterization of Stem Cells: Gene Expression
2.5. Metastable States of Stem Cells
2.6. Pluripotent Stem Cells
2.7. Multipotent Stem Cells
2.8. Stem Cells in Skin Epithelia
2.9. Stem Cells in the Intestine
2.10. Stem Cells in the Central Nervous System
2.11. Future Perspectives
2.12. Summary
Chapter 3. Tissue Formation during Embryogenesis
Learning Objectives
3.1. Introduction
3.2. Cardiac Development
3.3. Blood Vessel Development
3.4. Development of Peripheral Nerve Tissue
3.5. Embryonic Skin Development
3.6. Skeletal Formation
3.7. Future Directions
3.8. Summary
Chapter 4. Cellular Signaling
Learning Objectives
4.1. General Introduction
4.2. Cellular Signaling in Skin Biology
4.3. Cellular Signaling in Vascular Biology
4.4. Cellular Signaling in Bone Biology
4.5. Cellular Signaling in Skeletal Muscle
4.6. Future Developments
4.7. Snapshot Summary
Chapter 5. Extracellular Matrix as a Bioscaffold for Tissue Engineering
Learning Objectives
5.1. Introduction
5.2. Native Extracellular Matrix
5.3. ECM Scaffold Preparation
5.4. Constructive Tissue Remodeling
5.5. Clinical Translation of ECM Bioscaffolds
5.6. Commercially Available Scaffolds Composed of ECM
5.7. Future Considerations
5.8. Summary
Chapter 6. Degradation of Biomaterials
Learning Objectives
6.1. Degradable Bioceramics
6.2. Biodegradable Polymers
6.3. Future Perspectives for Degradable Biomaterials in Tissue Engineering
6.4. Summary
Chapter 7. Cell–Material Interactions
Learning Objectives
7.1. Introduction
7.2. Surface Chemistry
7.3. Surface Topography
7.4. Material Mechanics (Stiffness)
7.5. Summary
Chapter 8. Materiomics: A Toolkit for Developing New Biomaterials
Learning Objectives
8.1. Introduction: What is Materiomics?
8.2. Why Do We Need New Biomaterials
8.3. The Size of Chemical Space
8.4. Design of Experiments/Genetic Evolution/Parallels to Drug Discovery
8.5. High-Throughput Experimental Methods
8.6. Computational Modeling
8.7. Future Perspective
8.8. Summary
Chapter 9. Microfabrication Technology in Tissue Engineering
Learning Objectives
9.1. Introduction
9.2. Microfabrication Techniques in Tissue Engineering
9.3. Conclusion and Future Perspective
9.4. Summary
Chapter 10. Scaffold Design and Fabrication
Learning Objectives
10.1. Introduction
10.2. Scaffold Design
10.3. Classical Scaffold Fabrication Techniques
10.4. Electrospinning
10.5. Additive Manufacturing
10.6. Conclusion and Future Directions
Chapter 11. Controlled Release Strategies in Tissue Engineering
Learning Objectives
11.1. Introduction
11.2. Bioactive Factors Admixed with Matrices
11.3. Bioactive Factors Entrapped within Gel Matrices
11.4. Bioactive Factors Entrapped within Hydrophobic Scaffolds or Microparticles
11.5. Bioactive Factors Bound to Affinity Sites within Matrices
11.6. Bioactive Factors Covalently Bound to Matrices
11.7. Matrices Used for Immunomodulation
11.8. Summary
Chapter 12. Bioreactors: Enabling Technologies for Research and Manufacturing
Learning Objectives
12.1. Introduction
12.2. Enabling Tools for Tissue Engineers
12.3. Bioreactor-Based In vitro Model Systems
12.4. Bioreactors as Tissue Manufacturing Devices
12.5. Conclusions and Future Perspectives
12.6. Snapshot Summary
Chapter 13. Clinical Grade Production of Mesenchymal Stromal Cells
Learning Objectives
13.1. Introduction
13.2. Isolation of BM-MSCs
13.3. Culture Expansion
13.4. Characterization of Culture-Expanded MSCs
13.5. Cryopresentation
13.6. Production of Clinical Grade MSCs
13.7. Donor Variability and Donor-Related Parameters Affecting In Vitro Properties and Expansion Ability of MSCs
13.8. Relationship between In Vitro Assayed MSC Properties and Their Possible In Vivo Function
13.9. Future Perspectives
13.10. Snapshot Summary
Chapter 14. Vascularization, Survival, and Functionality of Tissue-Engineered Constructs
Learning Objectives
14.1. Introduction
14.2. Strategies to Improve Vascular Ingrowth into Tissue-Engineered Constructs
14.3. Prevascularization Strategies
14.4. Strategies to Improve Cell Survival
14.5. In vivo Models
14.6. Conclusion/Outlook
14.7. Summary
Chapter 15. Skin Engineering and Keratinocyte Stem Cell Therapy
Learning Objectives
15.1. Introduction
15.2. Structure of the Epidermis
15.3. Keratins
15.4. Structure of the Dermoepidermal Junction
15.5. In Vitro Keratinocyte Culture
15.6. Immunogenicity and Cultured Keratinocytes
15.7. Development of In Vivo Somatic Keratinocyte Stem Cell Grafting
15.8. Poor Keratinocyte “Take”
15.9. Enhanced Dermal Grafting
15.10. The Use of Adult Stem Cells in Tissue-Engineered Skin
15.11. The Future of Tissue-Engineered Skin
15.12. Summary
Chapter 16. Cartilage and Bone Regeneration
Learning Objectives
16.1. Introduction: Cartilage
16.2. Cellular Structures and Matrix Composition of Hyaline Cartilage
16.3. Collagen
16.4. Proteoglycans
16.5. The Chondrocyte
16.6. Stem Cells in Cartilage and Proliferation of Chondrocytes
16.7. Pathophysiology of Cartilage Lesion Development
16.8. Artificial Induction of Cartilage Repair
16.9. Rationale for Cell Implantation
16.10. Cartilage Specimens for Implantation
16.11. Cell Seeding Density
16.12. What Type of Chondrogenic Cells are Ideal for Cartilage Engineering?
16.13. Allogeneic versus Autologous Cells
16.14. Articular Chondrocytes versus Other Cells
16.15. Embryonic Stem Cells and Induced Pluripotent Stem Cells
16.16. Xenograft Cells
16.17. Direct Isolation of Tissue
16.18. Scaffolds in Cartilage Tissue Engineering
16.19. Bioreactors in Cartilage Tissue Engineering
16.20. Growth Factors that Stimulate Chondrogenesis
16.21. Future Developments in Cartilage Biology
16.22. Introduction: Bone—Basic Bone Biology: Structure, Function, and Cells
16.23. Bone Composition
16.24. Bone Formation
16.25. Intramembranous Ossification
16.26. Endochondral Ossification
16.27. Fracture Repair
16.28. Skeletal Stem Cells
16.29. Expansion and Differentiation
16.30. Growth Factors for Bone Repair
16.31. Scaffold Biocompatibility
16.32. The Function of the Vasculature in Skeletal Regeneration
16.33. Animal Models in Bone Tissue Engineering
16.34. Current Status of Bone Tissue Engineering
16.35. Future Perspectives for Bone Regeneration
16.36. Summary
Chapter 17. Tissue Engineering of the Nervous System
learning Objectives
17.1. Introduction
17.2. Peripheral Nerve
17.3. CNS: Spinal Cord
17.4. CNS: Optic Nerve
17.5. CNS: Retina
17.6. CNS: Brain
17.7. Neuroprostheses
17.8. Future Approaches
17.9. Summary
Chapter 18. Principles of Cardiovascular Tissue Engineering
Learning Objectives
18.1. Introduction
18.2. Heart Structure, Disease, and Regeneration
18.3. Cell Sources for Cardiovascular Tissue Engineering and Regeneration
18.4. Biomaterials—Polymers, Scaffolds, and Basic Design Criteria
18.5. Biomaterials as Vehicles for Stem Cells or Bioactive Molecule Delivery
18.6. Bioengineering of Cardiac Patches, In vitro
18.7. Vascularization of Cardiac Patches
18.8. Bioengineering of Blood Vessels
18.9. In situ Tissue Reconstruction by Injectable Acellular Biomaterials
18.10. Conclusions and Future Perspectives
18.11. Summary
Chapter 19. Tissue Engineering of Organ Systems
Learning Objectives
19.1. Introduction
19.2. Urogenital Tissue Engineering
19.3. Liver Tissue Engineering
19.4. Gastrointestinal Tissue Engineering
19.5. Pancreas Tissue Engineering
19.6. Lung Tissue Engineering
19.7. Future Developments
19.8. Summary
Chapter 20. Organs-on-a-Chip
Learning Objectives
20.1. Introduction
20.2. Concept of Organ-on-a-Chip
20.3. Examples of Organ-on-a-Chip
20.4. Conclusion
20.5. Summary
Chapter 21. Product and Process Design: Toward Industrial TE Manufacturing
Learning Objectives
21.1. Introduction
21.2. Bioreactor Systems for TE Product Manufacturing
21.3. Quality Control for TE Products—A Multiscale Approach
21.4. Online Data-Based Monitoring–Cross-Talk between Process Parameters and TE Construct Quality Attributes
21.5. Enhancing In Vivo Performance: An In Silico Mediated Approach for TE Product Design
21.6. Downstream Processing in TE Manufacturing
21.7. Toward Efficient TE Product Translation
21.8. Snapshot Summary
Chapter 22. Clinical Translation
Learning Objectives
22.1. Introduction
22.2. Clinical Translation of Tissue-Engineered Products
22.3. Typical Challenges for Tissue Engineering Encountered in the Clinical Phase
22.4. Implementation of a Clinical Trial
22.5. Special Points to Consider
22.6. Conclusion and Future Perspectives
22.7. Snapshot Summary
Chapter 23. Ethical Issues in Tissue Engineering
Learning Objectives
23.1. Introduction
23.2. Morality, Ethics, and Values
23.3. Moral Problems Relating to the Source of Material for Tissue Engineering
23.4. New Technologies: New Possibilities and New Dangers
23.5. Some Questions for the Future
23.6. Notes
Index
No. of pages: 896
Language: English
Edition: 2
Published: December 10, 2014
Imprint: Academic Press
Hardback ISBN: 9780124201453
eBook ISBN: 9780124202108
Cv
Clemens van Blitterswijk
Clemens van Blitterswijk graduated as cell biologist from Leiden University in 1982, defending his PhD thesis in 1985 at the same university. Today his research focuses on tissue engineering and regenerative medicine, forming a unique basis of multidisciplinary research between materials and life sciences. Van Blitterswijk has authored and co-authored more than 380 peer reviewed papers (H index 90, Scopus); is one of the most frequently cited Dutch scientists in TE; the applicant and co-applicant of over 100 patents; has guided 50 PhD candidates through their thesis as supervisor or co-supervisor and currently has 30 PhD candidates under his supervision. Dr. van Blitterswijk received a number of prestigious international awards including the George Winter award of the European society for Biomaterials, the Career Achievement Award of the Tissue Engineering and Regenerative Medicine International Society and is a member of the KNAW (The Royal Netherlands Academy of Arts and Sciences).
Affiliations and expertise
KNAW (The Royal Netherlands Academy of Arts and Sciences), The Netherlands
JD
Jan De Boer
Jan de Boer is an experienced University Professor and Chief Scientific Officer with a demonstrated history of working in academia and biotech. As a research professional he is skilled in Stem Cells, Biomaterial Engineering and Regenerative Medicine. Jan is interested in the molecular complexity of cells and how molecular circuits are involved in cell and tissue function. With a background in mouse and Drosophila genetics, he entered the field of biomedical engineering in 2002 and has since focused on understanding and implementing molecular biology in the field of tissue engineering and regenerative medicine. His research is characterized by a holistic approach to both discovery and application, aiming at combining high throughput technologies, computational modeling and experimental cell biology, to streamline the wealth of biological knowledge to real clinical applications.
Affiliations and expertise
Professor, Department of Biomedical Engineering, The Netherlands