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Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens - 1st Edition - ISBN: 9780126683301, 9780323138963

Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens

1st Edition

Volume Investigation of Biological Specimens

Series Editors: Dennis Buetow Ian Cameron G. Padilla A. Zimmerman
Series Volume Editors: John Stevens Linda Mills Judy Trogadis
eBook ISBN: 9780323138963
Imprint: Academic Press
Published Date: 1st September 1994
Page Count: 507
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Table of Contents

Confocal Microscopy: Practical Considerations:
Introduction to Confocal Three-Dimensional Volume Investigation.
Background Rejection and Optimization of Signal to Noise in Confocal Microscopy.
Sources of Noise in Three-Dimensional Microscopical Data Sets.
Simultaneous Ultraviolet and Visible Wavelength Confocal Microscopy.
Fluorescent Labels for Confocal Microscopy.
Display Methods for Gray-Scale, Voxel-Based Data Sets.
Three-Dimensional Volume Reconstitution in Confocal Microscopy: Practical Considerations.
Biological Applications of Confocal Microscopy:
Imagine Ion Channels in Live Central Neurons Using Fluorescent Ligands.
Imaging Ion Channels in Live Central Neurons Using Fluorescent Ligands II.
Labeling of Cells and Tissues.
Expression of G Protein-Coupled Receptors n Baculovirus/Sf9 Cells: Imaging Receptor Distribution by Confocal Fluorescence Microscopy.
Dynamic Intracellular Calcium Compartments: Confocal Microscopy Using Fluo-3 in Cells and Organelles.
Confocal Microscopy of Living Eggs and Embryos.
Spatial Organization of Microtubules in PC12 Cells: Three-Dimensional Electron Microscopy and Confocal Microscopy Applied to Volume Investigation.
Confocal Imaging of Living Neurons and Organelles.
Confocal Microscopy in Diagnostic Cytology.
Confocal Microscopy in Diagnostic Pathology.
Alternative Approaches and Future Applications:
Ultrathin Optical Sectioning and Dynamic Volume Investigation with Conventional Light Microscopy.
Serial Electron Microscopy as an Alternative or Complement to Confocal Microscopy for the Study of Synapses and Dendritic Spines in the Central Nervous System.
Confocal Scanning Opthalmoscopy: Applying Laser Scanning Technology to Retinal Imaging.
Use of Three-Dimensional Reconstructed Magnetic Resonance Imaging Data for Neurosurgical Planning.


The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems.

Key Features

  • Volume investigation
  • Three-dimensional reconstruction
  • Fluroescent probe design
  • Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology
  • Confocal data display and analysis
  • Twenty-eight pages of color


Cell biologists, molecular biologists, neurobiologists, and biomedical researchers


No. of pages:
© Academic Press 1994
1st September 1994
Academic Press
eBook ISBN:

Ratings and Reviews

About the Series Editors

Dennis Buetow

Affiliations and Expertise

University of Illinois

Ian Cameron

Ian Cameron is Professor in Chemical Engineering at the University of Queensland with teaching, research, and consulting activities in process systems engineering. He has a particular interest in process modelling, dynamic simulation, and the application of functional systems perspectives to risk management, having extensive industrial experience in these areas. He continues to work closely with industry and government on systems approaches to process and risk management issues. He received his BE from the University of New South Wales (Australia) and his PhD from imperial College London. He is a Fellow of IChemE.

Affiliations and Expertise

School of Chemical Engineering, University of Queensland, Brisbane, Australia

G. Padilla

Affiliations and Expertise

Duke University Medical Center, Durham, North Carolina, U.S.A.

A. Zimmerman

Affiliations and Expertise

University of Toronto, Ontario, Canada

About the Series Volume Editors

John Stevens

Affiliations and Expertise

Toronto Western Hospital and University of Toronto, Toronto, Ontario, Canada

Linda Mills

Affiliations and Expertise

Toronto Western Hospital and University of Toronto, Toronto, Ontario, Canada

Judy Trogadis

Affiliations and Expertise

Toronto Western Hospital and University of Toronto, Toronto, Ontario, Canada