Description

This volume, along with its companion (volume 475), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This first installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation, and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases.
The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

*Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling
* Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods
* Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Readership

Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.

Table of Contents

     

    1. His-tag switch method for the analysis of S-nitrosylated proteins
    2. Identification of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes
    3. Mitochondrial thioredoxin reductase: purification, inhibitor studies, and role in cell signaling
    4. assessing cell surface thiol status
    5. Induction of Thioredoxin for Mediating Preconditioning-induced Cellular Responses
    6. A trans sarcoplasmic reticulum membrane redox sensor in the striated muscle: exploring redox sensitivity of the ryanodine receptor calcium release channel
    7. Rapid approach for the detection, quantification and discovery of novel sulphenic acid or S-nitrosothiol modified proteins using a biotin-switch method
    8. Direct identification by mass spectrometry of in vivo S-nitrosylated peptides
    9. Changing paradigms in theology: From antioxidant defence to redox regulation
    10. Determination of GSNO formation in biological samples by HPLC electrochemical detection
    11. Alteration of thioredoxin reductase 1 levels in elucidating cancer etiology
    12. thiol-labeling technology in proteomics
    13. Analytical methods for the determination of sulfur metabolite concentrations in cell extracts
    14. Chemical tagging and mass spectrometry-based identification of protein thiols modified by lipid peroxidation-derived a,b-unsaturated aldehydes
    15. Measuring protein thiol redox changes in mitochondria
    16. Engineering of redox domains for monitoring electron transfers spectroscopically
    17. Approaches to detection of cysteine sulfenic acids in proteins using dimedone-based chemical probes
    18. Evaluation of conditions affecting degree of sulfenic acid

    Details

    No. of pages:
    416
    Language:
    English
    Copyright:
    © 2010
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780123813459
    Electronic ISBN:
    9780123813466