The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology

The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology

4th Edition - June 13, 2016

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  • Editors: H. Detrich, III, Monte Westerfield, Leonard Zon
  • eBook ISBN: 9780128052068
  • Hardcover ISBN: 9780128050552

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The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology, the second volume on the topic in the Methods in Cell Biology series, looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology.

Key Features

  • Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies
  • Chapters written by experts in the field
  • Contains cutting-edge material on the topic of zebrafish and developments relating to their cellular and developmental biology
  • New, two part Fourth Edition in this important volume


Researchers and students in cell, molecular and developmental biology

Table of Contents

  • Chapter 1. Methods to study maternal regulation of germ cell specification in zebrafish

    • Introduction
    • 1. Stages of Maternal Germ Plasm Regulation and Specification
    • 2. Studying the Dynamic Localization of Germ Plasm RNAs
    • 3. Studying Molecular Requirements Through Loss-of-Function Approaches
    • 4. Utility of Transgenic Fish to Study Germ Cell Development
    • Conclusion

    Chapter 2. Patterning, morphogenesis, and neurogenesis of zebrafish cranial sensory placodes

    • Introduction
    • 1. Specification of the PPR and Patterning the PPR into Specific Placodes
    • 2. Cranial Placode Morphogenesis and Neurogenesis
    • Conclusions

    Chapter 3. Oligodendrocyte differentiation

    • Introduction
    • 1. Zebrafish As a Model to Study Oligodendrocyte Differentation
    • 2. Insights Into Oligodendrocyte Development and Myelination Using Zebrafish
    • 3. Zebrafish As Model to Study Remyelination
    • Future Directions and Conclusions

    Chapter 4. Studying the peripheral sympathetic nervous system and neuroblastoma in zebrafish

    • Introduction
    • 1. The Peripheral Autonomic Nervous System
    • 2. The Zebrafish as a Model System for Studying PSNS Development
    • 3. Zebrafish as a Novel Model for Studying Neuroblastoma
    • Conclusion

    Chapter 5. Zebrafish as a model for understanding enteric nervous system interactions in the developing intestinal tract

    • Introduction
    • 1. Genetic and Environmental Interactions During Enteric Nervous System Development
    • 2. Interactions Between the Enteric Nervous System and the Immune System
    • 3. Interactions Between the Enteric Nervous System and Effector Cells
    • 4. Future Prospects

    Chapter 6. Methods to study the development, anatomy, and function of the zebrafish inner ear across the life course

    • Introduction
    • 1. Imaging Methods for Analysis of the Zebrafish Ear and Lateral Line
    • 2. Experimental Manipulation of Otic Development
    • 3. Dissection and Imaging of the Adult Ear and Otoliths
    • 4. Behavioral Analysis
    • 5. Small Molecule Screening
    • 6. Zebrafish Models of Human Deafness and Vestibular Disorders
    • 7. Future Directions

    Chapter 7. Imaging collective cell migration and hair cell regeneration in the sensory lateral line

    • Introduction
    • 1. Lateral Line Development
    • 2. In Vivo Imaging of Lateral Line Primordium Migration
    • 3. Live Labeling of Lateral Line Cells
    • 4. Nonvital Tissue Labeling of Lateral Line Cells
    • 5. Interpretation of Common Phenotypes
    • 6. Hair Cell Regeneration
    • 7. Long-Term Time-Lapse Analyses of Regenerating Neuromasts
    • Conclusions

    Chapter 8. Analysis of the retina in the zebrafish model

    • Introduction
    • 1. Development of the Zebrafish Retina
    • 2. Analysis of the Visual System in Wild Type and Mutants
    • 3. Analysis of Gene Function in the Zebrafish Retina
    • Summary

    Chapter 9. Strategies for analyzing cardiac phenotypes in the zebrafish embryo

    • Introduction
    • 1. Regulation of Heart Size
    • 2. Regulation of Cardiac Morphology
    • 3. Regulation of Cardiac Function
    • 4. Summary

    Chapter 10. Chemical approaches to angiogenesis in development and regeneration

    • Introduction
    • 1. High-Throughput Screening for Small Molecules With Proangiogenic Activity Using Primary Cell Culture of Tg[flk:GFP] Zebrafish Embryos
    • 2. Validation of Proangiogenic Compounds in Vivo Using Pre-inhibited Vascular Structure of Zebrafish Embryo by VRI
    • 3. Summary
    • 4. Method

    Chapter 11. Quantitative methods for studying hemostasis in zebrafish larvae

    • Introduction
    • 1. Methods
    • Conclusions

    Chapter 12. Zebrafish kidney development

    • Introduction
    • 1. Structure of the Zebrafish Pronephros
    • 2. Formation of the Pronephros
    • 3. Methods to Study Pronephros Function
    • Conclusions

    Chapter 13. Zebrafish pancreas as a model for development and disease

    • Introduction
    • 1. Molecular Mechanisms of Secondary Islet Formation
    • 2. Physiology and Assessment of Glucose Homeostasis in Zebrafish
    • 3. Diabetes and Related Disease Models in Zebrafish
    • 4. Methods to Study Beta Cell Biology and Physiology
    • 5. Future Directions

    Chapter 14. Endoderm specification and liver development

    • Introduction
    • 1. Review of the Literature
    • 2. Embryonic and Larval Protocols to Analyze Liver Formation
    • 3. Liver Injury and Regeneration Protocols
    • Summary

    Chapter 15. Emerging tools to study proteoglycan function during skeletal development

    • Introduction
    • 1. Xylose: Where the “Proteo-” meets the “-Glycan”
    • 2. Adding Sugar Like a Kid After Halloween
    • 3. Don't Be a Quitter: Posttranslational Modifications of Posttranslational Modifications
    • 4. Break it Down for Me, Fellas
    • 5. Adding Function to Structure by Understanding PG-Loss Animal Models
    • 6. Here's Looking at You, PG
    • Conclusion

    Chapter 16. Generation and analysis of zebrafish melanoma models

    • Introduction
    • 1. Genetic Models of Melanoma in Zebrafish
    • 2. Cell Line Models of Melanoma in Zebrafish
    • 3. Analysis of Melanomas
    • 4. Perspectives

    Chapter 17. Learning and memory in zebrafish (Danio rerio)

    • Introduction
    • Summary

    Chapter 18. Working with zebrafish at postembryonic stages

    • Introduction
    • 1. Part I: Postembryonic Staging
    • 2. Part II: Rearing Fish for Use at Postembryonic Stages
    • 3. Recipes

Product details

  • No. of pages: 660
  • Language: English
  • Copyright: © Academic Press 2016
  • Published: June 13, 2016
  • Imprint: Academic Press
  • eBook ISBN: 9780128052068
  • Hardcover ISBN: 9780128050552

About the Serial Volume Editors

H. Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Professor of Biochemistry and Marine Biology at Northeastern University

Monte Westerfield

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Affiliations and Expertise

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Leonard Zon

Grousbeck Professor of Pediatrics, Boston Children's Hospital / HHMI, Boston, MA, USA

Affiliations and Expertise

Boston Children's Hospital / HHMI, Boston, MA, USA

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