The Use Of Radioautography in Investigating Protein Synthesis

The Use Of Radioautography in Investigating Protein Synthesis

1st Edition - January 1, 1965

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  • Editor: C Leblond
  • eBook ISBN: 9780323154321

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Description

The Use of Radioautography in Investigating Protein Synthesis represents the 1965 Symposium on The Use of Radioautography in Investigating Protein Synthesis, supported by the International Society for Cell Biology. This book is composed of four parts encompassing 18 chapters. The opening part deals first with the basic aspects and mode of operation of the radioautography technique. This part also examines the advantages and potentials of this technique compared to other cytological techniques. The next parts are devoted to the experimental methods of protein synthesis in nucleus and in cells of various types, as revealed by the electron microscope radioautography. The concluding part discusses the secretion and synthesis of protein enzymes by the acinar cells in rat pancreas and fibroblasts in wound healing. This part also considers the application of radioautography to determining the sites of synthesis of proteins. This book is of great value to cell biologists, biochemists, cytologists, histologists, and pathologists.

Table of Contents


  • List of Contributors

    Preface

    Opening Address

    Techniques and Methods

    State of Light Autoradiography

    Assessment of Technical Steps in Electron Microscope Autoradiography

    Comparison of Emulsions and Techniques in Electron Microscope Radioautography

    Application of Freeze-Drying and Formaldehyde-Vapor Fixation to Radioautographic Localization of Soluble Amino Acids. By

    Protein Synthesis in Nucleus

    Interchange of Protein between Nucleus and Cytoplasm

    Amino Acid Deprivation and Deoxyribonucleic Acid Synthesis in Tetrahymena

    Cytoplasmic Protein Synthesis in Cells of Various Types and Its Relation to Nuclear Protein Synthesis

    Protein Synthesis in Cells of Various Types

    Protein Synthesis in Lymphocytes

    Fate of Newly Synthesized Proteins in Neurons

    Protein Synthesis in the Ear under Normal and Pathological Conditions

    Amino Acid Utilization in the Synthesis of Enamel and Dentin Matrices as Visualized by Autoradiography

    Protein Synthesis and Cells of the Skeletal System

    Synthesis and Turnover of Collagen in Periodontal Tissues

    Secretory Processes

    Secretion of Protein Enzymes by the Acinar Cells of the Rat Pancreas

    Synthesis and Secretion of Collagen by Fibroblasts in Healing Wounds

    The Formation of the Basement Lamella in Regenerating Salamander

    The Elaboration of Thyroglobulin by the Thyroid Follicular Cells

    What Radioautography Has Added to Protein Lore

    Author Index


Product details

  • No. of pages: 358
  • Language: English
  • Copyright: © Academic Press 1965
  • Published: January 1, 1965
  • Imprint: Academic Press
  • eBook ISBN: 9780323154321

About the Editor

C Leblond

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