Description

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

Key Features

This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights.

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

Section I: Measuring and Modifying the UPR

  1. Methods for investigating the UPR in filamentous fungi
  2. Thomas Guillemette, Arthur F.J. Ram, Neuza D.S.P. Carvalho, Aymeric Joubert, Philippe Simoneau, David B. Archer

  3. Assays for detecting the unfolded protein response
  4. Karen Cawley, Shane Deegan, Afshin Samali, Sanjeev Gupta

  5. Analysis of the role of nerve growth factor in promoting cell survival during endoplasmic reticulum stress in PC12 cells
  6. Koji Shimoke , Harue Sasaya, and Toshihiko Ikeuchi

  7. Measuring ER stress and the unfolded protein response using mammalian tissue culture system
  8. Christine M. Oslowski and Fumihiko Urano

  9. Real-Time Monitoring of Er Stress in Living Cells And Animals Using Estrap Assay
  10. Masanori Kitamura and Nobuhiko Hiramatsu

  11. HIV protease inhibitors induce endoplasmic reticulum stress and disrupt barrier integrity in intestinal epithelial cells
  12. Huiping Zhou

  13. Dexamethasone induction of a heat-stress response
  14. José A. Guerrero, Vicente Vicente, Javier Corral

  15. Detecting and Quantitating Physiological Endoplasmic Reticulum Stress
  16. Ling Qi, Liu Yang and Hui Chen

    Section II: Molecular Components of the UPR

  17. PI 3-Kinase Regulatory Subunits as Regulators of the Unfolded Protein Response
  18. Jonathon N. Winnay and C. Ronald Kahn

  19. The Emerging Role of Histone Deacetylases (HDACs) in UPR regulation
  20. Soumen Kahali, Bhaswati Sarcar & Prakash Chinnaiyan

  21. Immunohistochemical detection of Activating Transcription Factor 3, a hub of the cellular adaptive-response network
  22. Tsonwin Hai, Swati Jalgaonkar, Christopher C. Wolford, and Xin Yin

  23. Experimental approaches for elucidation of stress-sensing

Details

No. of pages:
432
Language:
English
Copyright:
© 2011
Published:
Imprint:
Academic Press
eBook ISBN:
9780123851154
Print ISBN:
9780123851147

About the editor

P. Michael Conn

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA