The Proteins Pt 1 - 3rd Edition - ISBN: 9780125163019, 9780323162111

The Proteins Pt 1

3rd Edition

Editors: Hans Neurath
eBook ISBN: 9780323162111
Imprint: Academic Press
Published Date: 28th April 1975
Page Count: 564
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Description

The Proteins, Volume I, Third Edition presents the procedures for the isolation and characterization of proteins and methods for the establishment of homogeneity.

This volume contains chapters that discuss molecular sieve techniques for protein analysis; the theory and practice of biospecific affinity chromatography; gel electrophoresis of proteins in sodium dodecyl sulfate; sedimentation analysis of proteins; the subunit structure of proteins; and the analysis of protein subunit structure by electron microscopy.

Protein chemists, medical and biological researchers, and students who require more knowledge of protein chemistry will find the book of great value.

Table of Contents


List of Contributors


Preface


Chapter 1. Molecular Sieve Methods of Analysis


I. General Introduction


II. Chromatographic Transport Methods


III. Equilibrium Partitioning Methods


IV. Determination of Molecular Size and Weight


V. Molecular Sieve Properties of Polydisperse Solutes


VI. Self-Association of Protein Subunits


VII. Association between Dissimilar Subunits


VIII. Binding of Small Molecules to Proteins


References


Chapter 2. Biospecific Affinity Chromatography and Related Methods


I. Introduction


II. Basic Principles


III. Nomenclature


IV. Matrices (Carriers)


V. Methods for Attachment of Ligands


VI. Adsorption-Desorption Procedures


VII. Applications


VIII. Concluding Remarks


References


Chapter 3. Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures


I. Introduction


II. Interaction of Proteins and SDS


III. Molecular Weight Determinations on SDS Gels


IV. Methods for Further Characterization of Proteins Separated on SDS Gels


References


Chapter 4. Sedimentation Analysis of Proteins


I. Introduction: The Role of Analytical Sedimentation Methods in Protein Chemistry


II. Moving-Boundary Sedimentation Transport


III. Zonal Sedimentation Transport in Density Gradients


IV. Sedimentation Equilibrium


V. Advances and Trends in Ultracentrifuge Technology


References


Chapter 5. Quaternary Structure of Proteins


I. Introduction


II. Stoichiometric Constitution


III. Geometric Arrangements of Identical Subunits


IV. Energe

Details

No. of pages:
564
Language:
English
Copyright:
© Academic Press 1975
Published:
Imprint:
Academic Press
eBook ISBN:
9780323162111

About the Editor

Hans Neurath

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