The Proteins Pt 1

The Proteins Pt 1

3rd Edition - January 1, 1975

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  • Editor: Hans Neurath
  • eBook ISBN: 9780323162111

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Description

The Proteins, Volume I, Third Edition presents the procedures for the isolation and characterization of proteins and methods for the establishment of homogeneity. This volume contains chapters that discuss molecular sieve techniques for protein analysis; the theory and practice of biospecific affinity chromatography; gel electrophoresis of proteins in sodium dodecyl sulfate; sedimentation analysis of proteins; the subunit structure of proteins; and the analysis of protein subunit structure by electron microscopy. Protein chemists, medical and biological researchers, and students who require more knowledge of protein chemistry will find the book of great value.

Table of Contents


  • List of Contributors

    Preface

    Chapter 1. Molecular Sieve Methods of Analysis

    I. General Introduction

    II. Chromatographic Transport Methods

    III. Equilibrium Partitioning Methods

    IV. Determination of Molecular Size and Weight

    V. Molecular Sieve Properties of Polydisperse Solutes

    VI. Self-Association of Protein Subunits

    VII. Association between Dissimilar Subunits

    VIII. Binding of Small Molecules to Proteins

    References

    Chapter 2. Biospecific Affinity Chromatography and Related Methods

    I. Introduction

    II. Basic Principles

    III. Nomenclature

    IV. Matrices (Carriers)

    V. Methods for Attachment of Ligands

    VI. Adsorption-Desorption Procedures

    VII. Applications

    VIII. Concluding Remarks

    References

    Chapter 3. Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

    I. Introduction

    II. Interaction of Proteins and SDS

    III. Molecular Weight Determinations on SDS Gels

    IV. Methods for Further Characterization of Proteins Separated on SDS Gels

    References

    Chapter 4. Sedimentation Analysis of Proteins

    I. Introduction: The Role of Analytical Sedimentation Methods in Protein Chemistry

    II. Moving-Boundary Sedimentation Transport

    III. Zonal Sedimentation Transport in Density Gradients

    IV. Sedimentation Equilibrium

    V. Advances and Trends in Ultracentrifuge Technology

    References

    Chapter 5. Quaternary Structure of Proteins

    I. Introduction

    II. Stoichiometric Constitution

    III. Geometric Arrangements of Identical Subunits

    IV. Energetics of Self-Assembly

    V. Intersubunit Communication

    VI. Functional Aspects

    VII. Conclusion

    References

    Chapter 6. Electron Microscopy of Proteins

    I. Introduction

    II. Relationship between the Electron Microscope Image and the Specimen

    III. Specimens

    IV. Interpretation and Analysis of Images

    V. Results

    VI. Recent Developments and Possible Future Needs

    References

    Author Index

    Subject Index

Product details

  • No. of pages: 564
  • Language: English
  • Copyright: © Academic Press 1975
  • Published: January 1, 1975
  • Imprint: Academic Press
  • eBook ISBN: 9780323162111

About the Editor

Hans Neurath

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