The Plasma Proteins - 1st Edition - ISBN: 9781483228105, 9781483258676

The Plasma Proteins

1st Edition

Isolation, Characterization, and Function

Editors: Frank W Putnam
eBook ISBN: 9781483258676
Imprint: Academic Press
Published Date: 1st January 1960
Page Count: 426
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Description

The Plasma Proteins, Volume I: Isolation, Characterization, and Function focuses on the reactions, properties, characteristics, and transformations of plasma proteins.

The selection first offers information on the fractionation and isolation of purified components by precipitation methods and electrophoretic and ultracentrifugal analysis of normal human serum. Discussions focus on correlation of electrophoretic and ultracentrifugal results, electrophoretic analytical methodology and results, parameters influencing protein solubility, and techniques for the separation of proteins by precipitation methods. The text then ponders on the chromatography of plasma proteins and chemical composition and molecular parameters of purified plasma proteins.

The manuscript elaborates on plasma albumin and macroglobulins and high molecular weight antibodies. Topics include immunological properties, physical and chemical properties of normal and pathological macroglobulins, purity, homogeneity, and variability, denaturation behavior, and sulfhydryl groups, mercaptalbumin, and the mercury dimer. The book then examines glycoproteins and metal-binding plasma proteins and cation transport.

The selection is a highly recommended reference for biochemists and clinicians interested in plasma proteins.

Table of Contents


Contributors to Volume I


Contributors to Volume II


Preface


List of Tables in Volume I


Contents of Volume II


1. Introduction


I. Scope


II. Historical


III. Development of Physicochemical Instrumentation and of Inclusive Fractionation Procedures


IV. Recent Advances


V. Trends for the Future


References


2. Fractionation and Isolation of Purified Components by Precipitation Methods


I. Introduction


II. Parameters Which Influence Protein Solubility


III. Specific Techniques for Separation of Proteins by Precipitation Methods


References


Appendix


3. Electrophoretic and Ultracentrifugal Analysis of Normal Human Serum


I. Introduction


II. Electrophoretic Analytical Methodology and Results


III. Ultracentrifugal Analytical Methods


IV. Preparatory Methods


V. Correlation of Electrophoretic and Ultracentrifugal Results


References


4. Chromatography of the Plasma Proteins


I. Introduction


II. Adsorbents


III. Elution


IV. Examination of the Effluent


V. Applications


References


5. Chemical Composition and Molecular Parameters of Purified Plasma Proteins


I. Delineation of the Purified Plasma Proteins


II. Chemical Composition


III. Molecular Parameters


References


6. Plasma Albumin


I. Introduction


II. Purity, Homogeneity, and Variability


III. Amphoteric Behavior


IV. Ion-Binding Behavior


V. Sulfhydryl Groups, Mercaptalbumin, and the Mercury Dimer


VI. Behavior at Low pH


VII. Denaturation Behavior


VIII. Summary Discussion and

Details

No. of pages:
426
Language:
English
Copyright:
© Academic Press 1960
Published:
Imprint:
Academic Press
eBook ISBN:
9781483258676

About the Editor

Frank W Putnam

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