The Plasma Proteins - 1st Edition - ISBN: 9781483228105, 9781483258676

The Plasma Proteins

1st Edition

Isolation, Characterization, and Function

Editors: Frank W Putnam
eBook ISBN: 9781483258676
Imprint: Academic Press
Published Date: 1st January 1960
Page Count: 426
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Description

The Plasma Proteins, Volume I: Isolation, Characterization, and Function focuses on the reactions, properties, characteristics, and transformations of plasma proteins.

The selection first offers information on the fractionation and isolation of purified components by precipitation methods and electrophoretic and ultracentrifugal analysis of normal human serum. Discussions focus on correlation of electrophoretic and ultracentrifugal results, electrophoretic analytical methodology and results, parameters influencing protein solubility, and techniques for the separation of proteins by precipitation methods. The text then ponders on the chromatography of plasma proteins and chemical composition and molecular parameters of purified plasma proteins.

The manuscript elaborates on plasma albumin and macroglobulins and high molecular weight antibodies. Topics include immunological properties, physical and chemical properties of normal and pathological macroglobulins, purity, homogeneity, and variability, denaturation behavior, and sulfhydryl groups, mercaptalbumin, and the mercury dimer. The book then examines glycoproteins and metal-binding plasma proteins and cation transport.

The selection is a highly recommended reference for biochemists and clinicians interested in plasma proteins.

Table of Contents


Contributors to Volume I

Contributors to Volume II

Preface

List of Tables in Volume I

Contents of Volume II

1. Introduction

I. Scope

II. Historical

III. Development of Physicochemical Instrumentation and of Inclusive Fractionation Procedures

IV. Recent Advances

V. Trends for the Future

References

2. Fractionation and Isolation of Purified Components by Precipitation Methods

I. Introduction

II. Parameters Which Influence Protein Solubility

III. Specific Techniques for Separation of Proteins by Precipitation Methods

References

Appendix

3. Electrophoretic and Ultracentrifugal Analysis of Normal Human Serum

I. Introduction

II. Electrophoretic Analytical Methodology and Results

III. Ultracentrifugal Analytical Methods

IV. Preparatory Methods

V. Correlation of Electrophoretic and Ultracentrifugal Results

References

4. Chromatography of the Plasma Proteins

I. Introduction

II. Adsorbents

III. Elution

IV. Examination of the Effluent

V. Applications

References

5. Chemical Composition and Molecular Parameters of Purified Plasma Proteins

I. Delineation of the Purified Plasma Proteins

II. Chemical Composition

III. Molecular Parameters

References

6. Plasma Albumin

I. Introduction

II. Purity, Homogeneity, and Variability

III. Amphoteric Behavior

IV. Ion-Binding Behavior

V. Sulfhydryl Groups, Mercaptalbumin, and the Mercury Dimer

VI. Behavior at Low pH

VII. Denaturation Behavior

VIII. Summary Discussion and Physiological Implications

References

7. γ-Globulin and Antibodies

I. Association of Antibodies with γ-Globulin

II. Physical Properties of γ-Globulin and Antibody

III. Chemical Properties

IV. Biological Properties

References

8. Macroglobulins and High Molecular Weight Antibodies

I. Introduction

II. 19 S Components of Normal Serum

III. Physical and Chemical Properties of Normal and Pathological Macroglobulins

IV. Immunological Properties

V. 19 S Antibodies

VI. Waldenström's Macroglobulinemia and Related Disorders

VII. Summary

References

9. Glycoproteins

I. Introduction

II. Nomenclature

III. Sugar Components in the Plasma Glycoproteins

IV. Multiplicity of the Plasma Glycoproteins

V. Chemical Structure of Plasma Glycoproteins

VI. Concluding Comments

References

10. Metal-Binding Plasma Proteins and Cation Transport

I. Introduction

II. Iron

III. Copper

IV. Zinc

V. Alkaline Earth Metals

References

Author Index

Subject Index

Details

No. of pages:
426
Language:
English
Copyright:
© Academic Press 1960
Published:
Imprint:
Academic Press
eBook ISBN:
9781483258676

About the Editor

Frank W Putnam