The Peptides Analysis, Synthesis, Biology

Modern Techniques of Conformational Structural, and Configurational Analysis

1st Edition - August 28, 1981
Editor: Erhard Gross
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 5 5 6 3 - 2

The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides… Read more

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The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides comprehensive and critical reviews of important developments in all areas of peptide research including analysis, synthesis, and biology. X-ray structure studies, amino acid analysis, and chiroptical analysis of configuration are discussed, along with solid-phase sequencing and ultramicroanalysis with the aid of fluorescence. This volume is comprised of six chapters and begins with an account of crystal structure analysis on molecules containing 2-12 peptide units, focusing on the variety of intramolecular hydrogen bonds, cis peptide units, and multiple conformation. Conformational changes upon complexation with metal ions are considered, together with the inclusion of solvents as integral parts of a molecular structure. The following chapters explore the conformations of insulin, glucagon, pancreatic polypeptide and related molecules, as well as the molecular biology of these hormones based on crystal structures; the usefulness of chiroptical techniques for determining the absolute configuration of amino acids and small peptides; and ultramicroanalysis of peptides and proteins by high performance liquid chromatography and fluorescence detection. The final chapter looks at the status and future potential of solid-phase sequencing. This book is intended as a reference for specialists, a guide for the novice, and a forum for investigators concerned with research on peptides and proteins.

List of Contributors

Preface

Nomenclature and Abbreviations

Contents of Previous Volumes

Chapter 1. X-Ray Analysis: Conformation of Peptides in the Crystalline State

I. Introductory Remarks

II. Cyclic Dipeptides

III. Peptides Containing Tri-, Tetra-, and Pentapeptide Rings

IV. Hexapeptide and Heptapeptide Rings

V. Cyclic Octapeptides to Pentadecapeptides

VI. Linear Peptides

VII. Geometry of the Peptide Unit

VIII. Intramolecular Hydrogen Bonds

IX. Summary

References

Chapter 2. Crystal Structure Analysis of the Larger Peptide Hormones

I. Introduction

II. Insulin and Proinsulin

III. Hormones Homologous to Insulin

IV. Glucagon

V. Pancreatic Polypeptide

VI. Placental and Pituitary Hormones

VII. Conclusion and Outlook

References

Chapter 3. Determination of the Absolute Configuration of α-Amino Acids and Small Peptides by Chiroptical Means

I. Introduction

II. Definitions: Optical Rotatory Dispersion and Circular Dichroism

III. Chiroptical Properties of Amino Acids

IV. Chiroptical Properties of Metal Complexes and Chromophoric Derivatives of α-Amino Acids

V. Chiroptical Properties of Oligopeptides

VI. Chiroptical Properties of Metal Complexes and Chromophoric Derivatives of Di- and Oligopeptides

VII. Solvent, Salt, and Temperature Effects

VIII. Instrumentation

IX. Calibration and Standards

X. Conclusion and Outlook

References

Chapter 4. Ultramicroanalysis of Peptides and Proteins by High-Performance Liquid Chromatography and Fluorescence Detection

I. Introduction

II. Detection

III. High-Performance Liquid Chromatography

IV. Applications

V. Conclusion

References

Chapter 5. Amino Acid Analysis of Peptides

I. Introduction

II. Historical

III. Experimental

IV. Concluding Remarks

References

Chapter 6. Solid-Phase Sequencing of Peptides and Proteins

I. Introduction

II. Chemistry of the Edman Degradation

III. Immobilization of Peptides

IV. Supports

V. Instrumentation

VI. Sequencing Strategies

VII. Scope and Limitations

References

Author Index

Subject Index