The Peptides Analysis, Synthesis, Biology

The Peptides Analysis, Synthesis, Biology

Modern Techniques of Conformational Structural, and Configurational Analysis

1st Edition - August 28, 1981

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  • Editor: Erhard Gross
  • eBook ISBN: 9780323155632

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The Peptides: Analysis, Synthesis, Biology: Volume 4: Modern Techniques of Conformational, Structural, and Configurational Analysis is an open-ended treatise that provides comprehensive and critical reviews of important developments in all areas of peptide research including analysis, synthesis, and biology. X-ray structure studies, amino acid analysis, and chiroptical analysis of configuration are discussed, along with solid-phase sequencing and ultramicroanalysis with the aid of fluorescence. This volume is comprised of six chapters and begins with an account of crystal structure analysis on molecules containing 2-12 peptide units, focusing on the variety of intramolecular hydrogen bonds, cis peptide units, and multiple conformation. Conformational changes upon complexation with metal ions are considered, together with the inclusion of solvents as integral parts of a molecular structure. The following chapters explore the conformations of insulin, glucagon, pancreatic polypeptide and related molecules, as well as the molecular biology of these hormones based on crystal structures; the usefulness of chiroptical techniques for determining the absolute configuration of amino acids and small peptides; and ultramicroanalysis of peptides and proteins by high performance liquid chromatography and fluorescence detection. The final chapter looks at the status and future potential of solid-phase sequencing. This book is intended as a reference for specialists, a guide for the novice, and a forum for investigators concerned with research on peptides and proteins.

Table of Contents

  • List of Contributors


    Nomenclature and Abbreviations

    Contents of Previous Volumes

    Chapter 1. X-Ray Analysis: Conformation of Peptides in the Crystalline State

    I. Introductory Remarks

    II. Cyclic Dipeptides

    III. Peptides Containing Tri-, Tetra-, and Pentapeptide Rings

    IV. Hexapeptide and Heptapeptide Rings

    V. Cyclic Octapeptides to Pentadecapeptides

    VI. Linear Peptides

    VII. Geometry of the Peptide Unit

    VIII. Intramolecular Hydrogen Bonds

    IX. Summary


    Chapter 2. Crystal Structure Analysis of the Larger Peptide Hormones

    I. Introduction

    II. Insulin and Proinsulin

    III. Hormones Homologous to Insulin

    IV. Glucagon

    V. Pancreatic Polypeptide

    VI. Placental and Pituitary Hormones

    VII. Conclusion and Outlook


    Chapter 3. Determination of the Absolute Configuration of α-Amino Acids and Small Peptides by Chiroptical Means

    I. Introduction

    II. Definitions: Optical Rotatory Dispersion and Circular Dichroism

    III. Chiroptical Properties of Amino Acids

    IV. Chiroptical Properties of Metal Complexes and Chromophoric Derivatives of α-Amino Acids

    V. Chiroptical Properties of Oligopeptides

    VI. Chiroptical Properties of Metal Complexes and Chromophoric Derivatives of Di- and Oligopeptides

    VII. Solvent, Salt, and Temperature Effects

    VIII. Instrumentation

    IX. Calibration and Standards

    X. Conclusion and Outlook


    Chapter 4. Ultramicroanalysis of Peptides and Proteins by High-Performance Liquid Chromatography and Fluorescence Detection

    I. Introduction

    II. Detection

    III. High-Performance Liquid Chromatography

    IV. Applications

    V. Conclusion


    Chapter 5. Amino Acid Analysis of Peptides

    I. Introduction

    II. Historical

    III. Experimental

    IV. Concluding Remarks


    Chapter 6. Solid-Phase Sequencing of Peptides and Proteins

    I. Introduction

    II. Chemistry of the Edman Degradation

    III. Immobilization of Peptides

    IV. Supports

    V. Instrumentation

    VI. Sequencing Strategies

    VII. Scope and Limitations


    Author Index

    Subject Index

Product details

  • No. of pages: 330
  • Language: English
  • Copyright: © Academic Press 1981
  • Published: August 28, 1981
  • Imprint: Academic Press
  • eBook ISBN: 9780323155632

About the Editor

Erhard Gross

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