The Pentose Phosphate Pathway

The Pentose Phosphate Pathway

1st Edition - January 28, 1985

Write a review

  • Author: Terry Wood
  • eBook ISBN: 9780323160018

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order

Description

The Pentose Phosphate Pathway aims to explore the pentose phosphate cycle and the practical techniques applied in its investigation. The main focus of the book is the pentose phosphate cycle in animals as well as microorganisms, and does not discuss the one related to photosynthesis. The book covers the formulation of the pathway, its types, and its alternative formulations; the preparation, processes, and analysis of the pathway; and the enzymes involved. Also covered in the book are the intermediates in intact cells and tissues; distribution of enzymes among different tissues and species; the operation, regulation, and overall control of the pathway; and the clinical, nutritional, and metabolic aspects of the pathway. The text is recommended for biologists and biochemists who would like to understand further the pentose phosphate pathway and the processes related to it.

Table of Contents


  • Contents

    Preface

    List of Abbreviations

    1 Introduction

    Introduction

    2 Formulation of the Pathway

    Introduction

    Experiments with Isotopes

    The L-Type Pathway

    Other Alternative Formulations of the Pathway

    D-Erythrose 4-Phosphate as an Intermediate of the Pathway

    The Transketolase-Transaldolase Coupled Reaction and the Existence of Enzyme Complexes

    Conclusion

    3 Preparation, Properties, and Analysis of the Intermediates of the Pathway

    Introduction

    General Analytical Methods

    Preparation and General Chemistry

    Individual Intermediates

    4 Preparation, Properties, and Analysis of the Enzymes of the Pathway

    Introduction

    D-Ribose-5-Phosphate Ketol-Isomerase (EC 5.3.1.6)

    D-Ribulose-5-Phosphate 3-Epimerase (EC 5.1.3.1)

    Transaldolase (EC 2.2.1.2)

    Transketolase (EC 2.2.1.1)

    Other Enzymes

    5 Intermediates in Intact Cells and Tissues

    Introduction

    Liver

    Brain

    Erythrocytes

    Other Tissues

    Ascites and HeLa Cells

    Bacteria

    Plants

    Individual Intermediates

    6 Distribution of the Enzymes among Different Tissues and Different Species

    Introduction

    General Surveys

    Specific Tissues

    Measurements on Other Species

    Changes in Enzyme Levels during Development

    Discussion

    7 Operation and Regulation in Broken-Cell Preparations

    Introduction

    The Utilization of Different Substrates

    8 Operation and Regulation in Intact Cells and Tissues

    Introduction

    Factors Stimulating the Oxidative Branch of the Pathway

    The Effects of Changes in Physiological Factors on the Pathway

    9 Overall Control of the Pathway

    Introduction

    Approach I: Rate-Limiting Enzymes

    Approach II: Mass Action Ratios

    Approach III: Cross-Over Plots

    Approach IV: Enzymes under Hormonal and Allosteric Control

    Approach V: Enzymes That Change in Response to Changes in the Environment

    Control of the Oxidative Branch of the Pathway by an Activating Factor

    Mathematical Models

    Conclusion

    10 Clinical and Nutritional Aspects

    The Assay of Blood Transketolase

    Normal Values for Blood Transketolase

    The "TPP Effect"

    Measurements of Other Enzymes

    Clinical Studies of Various Pathological and Physiological States

    Studies of Transketolase and Thiamine Deficiency in Other Species

    The Mechanism of Activation of Transketolase by Thiamine

    11 Glutathione Metabolism and the Pentose Phosphate Pathway

    Text

    12 Use of Radioisotopes in Elucidating the Nature of and Quantitating the Pentose Pathway

    Introduction

    The Nature of the Pathway

    Quantitation

    Conclusion

    References

    Index

Product details

  • No. of pages: 216
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: January 28, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323160018

About the Author

Terry Wood

Ratings and Reviews

Write a review

There are currently no reviews for "The Pentose Phosphate Pathway"