The Natural History of Rabies, Volume 1 - 2nd Edition - ISBN: 9780120724017, 9780323139700

The Natural History of Rabies, Volume 1

2nd Edition

Authors: George M. Baer
eBook ISBN: 9780323139700
Imprint: Academic Press
Published Date: 28th January 1975
Page Count: 620
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The Natural History of Rabies, Volume I explores the fundamental aspects of the rabies virus, including its growth, latency, morphology, chemistry, physical characteristics, and relationships with other viruses. It looks at the virus' in vivo pathogenesis and pathology, from entrance to transmission in the central nervous system (CNS) and subsequent exit. It also reviews current diagnostic methods including those used for antibody titration and for determination of virus presence.

Organized into three sections encompassing 21 chapters, this volume begins with an overview of the history of rabies as well as its morphology and morphogenesis. It then discusses the virus' antigenic composition and relationships, hemagglutinin and the optimal conditions for its preparation and demonstration, advantages and disadvantages of the passive hemagglutination test, methods for concentration and purification of the virus, and growth in cell culture. It explains the virus' pathogenesis to and spread within and from the CNS, electron microscopy of CNS and extraneural rabies infection, lipotropism in rabies virus infection, use of exogenous and endogenous interferon to inhibit rabies virus infection, mouse inoculation and Negri body diagnosis, and fluorescent antibody test in rabies. The book concludes with an assessment of the serum neutralization, indirect fluorescent antibody, and rapid fluorescent focus inhibition tests.

This book is a valuable resource for virologists, pathologists, epidemiologists, and students.

Table of Contents

List of Contributors


Contents of Volume II

Chapter 1 History of Rabies



Part I The Virus

Chapter 2 Morphology and Morphogenesis

I. Morphology of Rabies Virus

II. Morphogenesis of Rabies Virus

III. The Bearing of Morphology and Morphogenesis on the Placement of Rabies Virus in the Rhabdovirus Group


Chapter 3 Antigenic Composition of Rabies Virus

I. Introduction

II. Rabies Antigen in Infected Cells

III. Antigens of the Rabies Virion

IV. Antigens of Disrupted Virions

V. Soluble Antigen

VI. Conclusion


Chapter 4 Chemical Composition and Structure of Rabies Virus

I. Introduction

II. Properties of the Virion

III. Disruption of the Virus into Its Components

IV. The Viral RNA

V. Viral Structural Proteins

VI. Intracellular Virus and Viral Components


Chapter 5 Hemagglutinin of Rabies Virus

I. Introduction

II. Optimal Conditions for Preparation and Demonstration of Hemagglutinin

III. Characteristics of Hemagglutinin

IV. Relationship between Hemagglutinating and Immunizing Capacity of Rabies Virus Particles

V. Hemagglutination Inhibition Test


Chapter 6 Passive Hemagglutination Test

I. Passive Hemagglutination Procedure

II. Advantages of the Passive Hemagglutination Test

III. Disadvantages of the Test


Chapter 7 Concentration and Purification

I. Introduction

II. Methods for Concentration

III. Methods of Purification

IV. Summary


Chapter 8 Rabies Virus Antigenic Relationships

I. Introduction

II. Antigenic Distinction between the Viruses of the Rabies Sero Group and Other Rhabdoviruses

III. Rabies-Related Viruses

IV. Antigenic Relationships in the Rabies Group

V. Other Antigenic Variants of Rabies Virus

VI. Significance of Rabies-Related Viruses


Part II Pathogenesis and Pathology

Chapter 9 Growth of Rabies Virus in Cell Culture

I. Introduction

II. Historical Review

III. Adaptation of Rabies Virus to Growth in Cell Culture

IV. Chronic Infection of Cell Culture Systems

V. Dynamics of Virus Replication in Vitro

VI. Virus-Specific Products of Infected Cells

VII. Virion-free Rabies Antigen (Soluble Antigen) (VFRA)

VIII. Immune Lysis

IX. Production of Interferon in Rabies Virus-Infected Cells

X. Cytopathic Effect of Rabies Virus in Cell Cultures

XI. Optimal Conditions for the Production of Rabies Virus in High Titer

XII. Coinfection of Cells in Vitro with Rabies and Other Viruses

XIII. Effect of Metabolic Inhibitors on Virus Replication in Vitro

XIV. Alteration of Rabies Virus Phenotype as a Result of Passage in Cell Culture


Chapter 10 Pathogenesis to the Central Nervous System

I. Pathogenesis after Subcutaneous or Intramuscular Introduction of Virus

II. Pathogenesis after Intranasal Introduction of Virus

III. Pathogenesis after Oral Administration

IV. Pathogenesis after Intravenous Inoculation of Virus


Chapter 11 Spread of Virus within the Central Nervous System

I. Demonstration of Infectivity

II. Histopathology

III. Immunofluorescence and Electron Microscopy

IV. Clinical Signs

V. Pathogenesis of Abortive and Chronic Infection

VI. Summary and Prospects


Chapter 12 Electron Microscopy of Central Nervous System Infection



Chapter 13 The Pathology of Rabies in the Central Nervous System

I. Microscopic Changes

II. Paralytic Rabies

III. Ultrastructural Studies


Chapter 14 Spread of Virus from the Central Nervous System

I. Introduction

II. Virus Isolations from Peripheral Nerves and Non-nervous Organs

III. Cells Involved in Virus Replication

IV. Intravitam Diagnostic Tests

V. Prevention of Virus Spread


Chapter 15 Electron Microscopy of Extraneural Rabies Infection

I. Extraneural Infection Prior to Invasion of the Nervous System

II. Extraneural Infection Following Centrifugal Spread from Nervous Tissue


Chapter 16 Lipotropism in Rabies Virus Infection



Chapter 17 Latency and Abortive Rabies

I. Definition and Historical Background

II. Criteria and Evidence

III. Mechanisms of Abortion

IV. Conditions Conducive to Nonfatal Infection

V. Implications of Nonfatal Infection (The Carrier State)

VI. Conclusions


Chapter 18 Interferon and Rabies Virus Infection

I. Homologous and Heterologous Interference with Rabies Virus

II. Inhibition of Rabies Virus Infection by Exogenous Interferon

III. Inhibition of Rabies Virus Infection by Endogenous Interferon

IV. Rabies Virus Strain Differences and Sensitivity to Interferon

V. Induction of Interferon by Rabies Vaccines


Part III Diagnosis

Chapter 19 Animal Inoculation and the Negri Body

I. Introduction

II. Animal Inoculation

III. The Mouse Inoculation Technique

IV. Negri Body Diagnosis


Chapter 20 The Fluorescent Antibody Test in Rabies

I. History

II. The Principle of the Fluorescent Antibody Technique

III. Selection of the Preferred Fluorochrome

IV. Microscopes and Light Sources Used in the FA Test

V. Fluorescent Antibody Techniques

VI. The FA Technique in Rabies


Chapter 21 The Serum Neutralization, Indirect Fluorescent Antibody, and Rapid Fluorescent Focus Inhibition Tests

I. Introduction

II. Rabies Antibody

III. Relation of Neutralizing Antibody to Protection

IV. Experiments Employing Serum Neutralization Tests

V. Performance of the Mouse Serum Neutralization Test

VI. Indirect Fluorescent Rabies Antibody Test

VII. Performance of the Indirect Fluorescent Rabies Antibody Test

VIII. Rabies Antibody Determination by Tissue Culture

IX. Fluorescent Focus Inhibition Test

X. Rapid Fluorescent Focus Inhibition Test

XI. Performance of the RFFIT

XII. Summary


Author Index

Subject Index


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George M. Baer

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