The Molecular Biology of the Bacilli

The Molecular Biology of the Bacilli

1st Edition - July 1, 1985

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  • Editor: David Dubnau
  • eBook ISBN: 9780323158190

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The Molecular Biology of the Bacilli: Volume II is a collection of material relevant to the basic knowledge of the bacillus system and to the development of the bacillus system for industrial applications. This volume presents bacillus research studies on the unique characteristics of bacilli and its interesting comparisons with other bacteria, specifically Escherichia coli. The first four chapters focus on the Bacillus subtilis, specifically the translational specificity, DNA repair, chemotaxis, and sporulation. Chapter 5 discusses the protoplast fusion in bacillus while Chapter 6 delves on the secretion of proteins by bacilli. Chapter 7 explores the function of bacilli as insecticide. Bacillus thuringiensis is also mentioned in this chapter. This topic is further discussed in Chapter 8 where the genetics and molecular biology of B. thuringiensis are discussed. This volume aims to be of help to students and researchers in various fields of biochemistry, genetics, biological sciences, and microbiology.

Table of Contents

  • Contributors


    1 Translational Specificity in Bacillus subtilis

    I. Introduction

    II. Translational Machinery

    III. A Hypothesis for Translational Specificity

    IV. Test of the "Simple Hypothesis" for Translational Specificity

    V. Conclusions


    2 DNA Repair in Bacillus subtilis

    I. Introduction

    II. Review of DNA Repair Systems

    III. Characterization of the DNA Repair Systems of Bacillus subtilis

    IV. Characterization of the "SOB" System

    V. Correlation Between the "SOB" System and the Development of the Competent State in Bacillus subtilis

    VI. Conclusions


    3 Chemotaxis in Bacillus subtilis

    I. Introduction

    II. Chemotaxis Experiments

    III. Biochemistry

    IV. Genetics

    V. Comparison with Chemotaxis in Escherichia coli

    VI. Mechanism of Chemotaxis


    4 Sporulation of Bacillus subtilis

    I. Introduction

    II. Start to Septation (Stages 0 to II)

    III. Septation to Engulfment (Stages II and II)

    IV. Engulfment to the Mature Spore (Stages III to VIII)

    V. Gene Expression


    5 Protoplast Fusion in Bacillus and Its Consequences

    I. Introduction

    II. Methodology

    III. Quantitative Assay of Fusion

    IV. The Nature of Fusion

    V. Recombination in Fusion Diploids

    VI. Stability and Instability of the Fusion Diploids

    VII. Genome Expression in Diploid Bacillus subtilis

    VIII. Fusion in Other Strains, Variants, and Species

    IX. Models of Diploid Chromosome Management

    X. Conclusions and Prospects


    6 Secretion of Proteins by Bacilli

    I. Introduction

    II. Types of Proteins Secreted by Bacilli

    III. Mechanisms of Secretion in Bacilli

    IV. Processing and Release

    V. Bacilli as Hosts for Genetically Engineered Products

    VI. Future Directions


    7 Insecticidal Bacilli

    I. Introduction

    II. Bacillus thuringiensis

    III. Bacillus popilliae and B. lentimorbus

    IV. Bacillus sphaericus and B. montai

    V. Summary


    8 The Genetics and Molecular Biology of Bacillus thuringiensis

    I. Introduction

    II. Detection and Characterization of Bacillus thuringiensis Plasmids

    III. Plasmids and δ-Endotoxin Synthesis

    IV. Molecular Cloning of the Toxin Genes

    V. Localization of Toxin Genes

    VI. Other Developments in Transformation and Transduction of Bacillus thuringiensis

    VII. Concluding Remarks



Product details

  • No. of pages: 276
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: July 1, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323158190

About the Editor

David Dubnau

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