The Isolated hepatocyte

The Isolated hepatocyte

Use in Toxicology and Xenobiotic Biotransformations

1st Edition - September 14, 1987

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  • Editor: E.J. Rauckman
  • eBook ISBN: 9780323156790

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Description

The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations covers the link between research on the isolated hepatocyte and the disciplines of cell culture, toxicology, metabolism, and molecular biology. This book is composed of 11 chapters and begins with an overview of the regulation of liver growth, sulfation, glucuronidation of xenobiotics in specific liver sites. The next chapters deal with toxicology studies in cultured hepatocytes from various species and the in vitro control of hepatocyte proliferation. These topics are followed by discussions of choices and methods of cytotoxicity measures; hepatoxicity of metals; the metabolism and toxicity of xenobiotics in a primary culture; and the mechanism of carcinogen-induced pleiotropic drug resistance in hepatocytes. The concluding chapters describe the in vivo and in vitro measurement of chemically-induced DNA repair in hepatocytes, as well as the genotoxicity studies with human hepatocytes. This book is intended primarily to toxicologists and researchers.

Table of Contents


  • Preface

    1 Regulation of Liver Growth: Historical Perspectives and Future Directions

    I. Introduction

    II. Whole-Animal Studies

    III. Hepatocyte Cultures

    IV. Future Directions

    References

    2 Regulation of Sulfation and Glucuronidation of Xenobiotics in Periportal and Pericentral Regions of the Liver Lobule

    I. Introduction

    II. Sulfation and Glucuronidation in Periportal and Pericentral Regions of the Liver Lobule

    III. Conclusion

    References

    3 Toxicology Studies in Cultured Hepatocytes from Various Species

    I. Introduction

    II. Hepatocyte Primary Cultures: Methods

    III. Uses of Hepatocyte Primary Cultures for Xenobiotic Studies

    IV. Conclusions

    References

    4 Cytochrome P-450-Dependent Monooxygenase Systems in Mouse Hepatocytes

    I. Introduction

    II. Methods Used to Study Drug Biotransformation in Isolated Hepatic Cells

    III. Applications

    IV. Conclusions

    References

    5 Control of Hepatocyte Proliferation in Vitro

    I. Introduction

    II. Hepatocyte Isolation and Culture

    III. Epidermal Growth Factor

    IV. Norepinephrine

    V. Factors Derived from Serum or Platelets

    VI. Factors Derived from Normal Liver Cells

    VII. Factors Derived from Neoplastic Liver Cells

    References

    6 Cytotoxicity Measures: Choices and Methods

    I. Introduction

    II. Plasma Membrane Integrity

    III. Subcellular Effects

    IV. Data Presentation

    V. In Vivo/in Vitro Correspondence

    VI. Methods

    References

    7 Metals, Hepatocytes, and Toxicology

    I. Introduction

    II. Uptake and Efflux of Metals

    III. Use of Hepatocytes to Study Chelators

    IV. Toxicity of Metals

    V. Interactions among Metals

    VI. Mechanisms of Toxicity

    VII. Conclusion

    References

    8 The Metabolism and Toxicity of Xenobiotics in a Primary Culture System of Postnatal Rat Hepatocytes

    I. Introduction

    II. Retention and Maintenance of Liver-Specific Differentiated Functions in Primary Culture of Postnatal Rat Hepatocytes

    III. Toxicity Evaluation of Xenobiotics in Cultured Hepatocytes

    IV. Metabolic Activation and Toxicity of Xenobiotics in Cultured Hepatocytes

    V. Conclusions

    References

    9 The Analysis of Carcinogen-Induced Pleiotropic Drug Resistance in Rat Hepatocytes

    I. Introduction

    II. Study of Drug Resistance in Carcinogen-Altered Hepatocytes

    III. Cytotoxicity Studies in Vitro

    IV. Cellular Pharmacokinetics of Adriamycin in Sensitive and Resistant Hepatocytes

    V. Membrane Actions of Adriamycin

    VI. Conclusions

    References

    10 Measurement of Chemically Induced DNA Repair in Hepatocytes in Vivo and in Vitro as an Indicator of Carcinogenic Potential

    I. Introduction

    II. DNA Repair

    III. Procedures for in Vitro and in Vivo Rat Hepatocyte DNA Repair Assays

    References

    11 Genotoxicity Studies with Human Hepatocytes

    I. Introduction

    II. Isolation and Culture of Human Hepatocytes

    III. Hepatocyte Cell Culture

    IV. Cryopreservation of Human Hepatocytes

    V. Genotoxicity Studies with Human Hepatocytes

    VI. Xenobiotic Metabolism by Human Hepatocytes

    VII. Conclusions

    References

    Index

Product details

  • No. of pages: 304
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: September 14, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9780323156790

About the Editor

E.J. Rauckman

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