The Isolated hepatocyte - 1st Edition - ISBN: 9780125828703, 9780323156790

The Isolated hepatocyte

1st Edition

Use in Toxicology and Xenobiotic Biotransformations

Editors: E.J. Rauckman
eBook ISBN: 9780323156790
Imprint: Academic Press
Published Date: 14th September 1987
Page Count: 304
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The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations covers the link between research on the isolated hepatocyte and the disciplines of cell culture, toxicology, metabolism, and molecular biology.

This book is composed of 11 chapters and begins with an overview of the regulation of liver growth, sulfation, glucuronidation of xenobiotics in specific liver sites. The next chapters deal with toxicology studies in cultured hepatocytes from various species and the in vitro control of hepatocyte proliferation. These topics are followed by discussions of choices and methods of cytotoxicity measures; hepatoxicity of metals; the metabolism and toxicity of xenobiotics in a primary culture; and the mechanism of carcinogen-induced pleiotropic drug resistance in hepatocytes. The concluding chapters describe the in vivo and in vitro measurement of chemically-induced DNA repair in hepatocytes, as well as the genotoxicity studies with human hepatocytes.

This book is intended primarily to toxicologists and researchers.

Table of Contents


1 Regulation of Liver Growth: Historical Perspectives and Future Directions

I. Introduction

II. Whole-Animal Studies

III. Hepatocyte Cultures

IV. Future Directions


2 Regulation of Sulfation and Glucuronidation of Xenobiotics in Periportal and Pericentral Regions of the Liver Lobule

I. Introduction

II. Sulfation and Glucuronidation in Periportal and Pericentral Regions of the Liver Lobule

III. Conclusion


3 Toxicology Studies in Cultured Hepatocytes from Various Species

I. Introduction

II. Hepatocyte Primary Cultures: Methods

III. Uses of Hepatocyte Primary Cultures for Xenobiotic Studies

IV. Conclusions


4 Cytochrome P-450-Dependent Monooxygenase Systems in Mouse Hepatocytes

I. Introduction

II. Methods Used to Study Drug Biotransformation in Isolated Hepatic Cells

III. Applications

IV. Conclusions


5 Control of Hepatocyte Proliferation in Vitro

I. Introduction

II. Hepatocyte Isolation and Culture

III. Epidermal Growth Factor

IV. Norepinephrine

V. Factors Derived from Serum or Platelets

VI. Factors Derived from Normal Liver Cells

VII. Factors Derived from Neoplastic Liver Cells


6 Cytotoxicity Measures: Choices and Methods

I. Introduction

II. Plasma Membrane Integrity

III. Subcellular Effects

IV. Data Presentation

V. In Vivo/in Vitro Correspondence

VI. Methods


7 Metals, Hepatocytes, and Toxicology

I. Introduction

II. Uptake and Efflux of Metals

III. Use of Hepatocytes to Study Chelators

IV. Toxicity of Metals

V. Interactions among Metals

VI. Mechanisms of Toxicity

VII. Conclusion


8 The Metabolism and Toxicity of Xenobiotics in a Primary Culture System of Postnatal Rat Hepatocytes

I. Introduction

II. Retention and Maintenance of Liver-Specific Differentiated Functions in Primary Culture of Postnatal Rat Hepatocytes

III. Toxicity Evaluation of Xenobiotics in Cultured Hepatocytes

IV. Metabolic Activation and Toxicity of Xenobiotics in Cultured Hepatocytes

V. Conclusions


9 The Analysis of Carcinogen-Induced Pleiotropic Drug Resistance in Rat Hepatocytes

I. Introduction

II. Study of Drug Resistance in Carcinogen-Altered Hepatocytes

III. Cytotoxicity Studies in Vitro

IV. Cellular Pharmacokinetics of Adriamycin in Sensitive and Resistant Hepatocytes

V. Membrane Actions of Adriamycin

VI. Conclusions


10 Measurement of Chemically Induced DNA Repair in Hepatocytes in Vivo and in Vitro as an Indicator of Carcinogenic Potential

I. Introduction

II. DNA Repair

III. Procedures for in Vitro and in Vivo Rat Hepatocyte DNA Repair Assays


11 Genotoxicity Studies with Human Hepatocytes

I. Introduction

II. Isolation and Culture of Human Hepatocytes

III. Hepatocyte Cell Culture

IV. Cryopreservation of Human Hepatocytes

V. Genotoxicity Studies with Human Hepatocytes

VI. Xenobiotic Metabolism by Human Hepatocytes

VII. Conclusions




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© Academic Press 1987
Academic Press
eBook ISBN:

About the Editor

E.J. Rauckman

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